##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AccIII      AclI        AflII       AscI        AseI        
AsnI        AvrII       BclI        BfrI        BlnI        
BsaI        BseAI       BspEI       BspHI       BspLU11I    
Bst1107I    BstXI       BstZ17I     CpoI        DrdI        
EcoRV       EcoT22I     FbaI        HincII      HindII      
HpaI        MfeI        MluI        MroI        MunI        
NdeI        NheI        NruI        NsiI        PacI        
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        ScaI        
SfiI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AflIII       1667  ACGTGT               A^C[GA][CT]GT
AgeI         1824  ACCGGT               A^CCGGT
BbrPI        2344  CACGTG               CAC^GTG
BciVI        3531  GGATAC               GTATCC(6/5)
BsaBI        4740  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI        2778  CGTACG               C^GTACG
BspDI        5334  ATCGAT               AT^CGAT
BssHII       5592  GCGCGC               G^CGCGC
BstBI        3301  TTCGAA               TT^CGAA
ClaI         5334  ATCGAT               AT^CGAT
DraI         6336  TTTAAA               TTT^AAA
FseI         1588  GGCCGGCC             GGCCGG^CC
HindIII      5446  AAGCTT               A^AGCTT
MamI         4740  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI         4879  GCGGCCGC             GC^GGCCGC
NspV         3301  TTCGAA               TT^CGAA
PaeR7I       4912  CTCGAG               C^TCGAG
PinAI        1824  ACCGGT               A^CCGGT
PmaCI        2344  CACGTG               CAC^GTG
PmlI         2344  CACGTG               CAC^GTG
SfuI         3301  TTCGAA               TT^CGAA
SgrAI        1823  CACCGGTG             C[GA]^CCGG[CT]G
XhoI         4912  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AatII        3857  GACGTC               GACGT^C
             5401  GACGTC              
Acc65I       3634  GGTACC               G^GTACC
             3991  GGTACC              
AccI         1831  GTCTAC               GT^[AC][GT]AC
             5128  GTCTAC              
Aor51HI      1231  AGCGCT               AGC^GCT
             3821  AGCGCT              
Asp718I      3634  GGTACC               G^GTACC
             3991  GGTACC              
AviII        1712  TGCGCA               TGC^GCA
             3131  TGCGCA              
BamHI         305  GGATCC               G^GATCC
             1985  GGATCC              
BglII         884  AGATCT               A^GATCT
             5354  AGATCT              
BsmBI         491  GAGACG               CGTCTC(1/5)
             5698  GAGACG              
BsmI          283  GAATGC               GAATGC(1/-1)
             3394  GCATTC              
BsrBI         978  CCGCTC               CCGCTC(-3/-3)
              634  GAGCGG              
DraIII       2341  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3169  CACTACGTG           
Eco47III     1231  AGCGCT               AGC^GCT
             3821  AGCGCT              
EcoRI         241  GAATTC               G^AATTC
             3139  GAATTC              
FspI         1712  TGCGCA               TGC^GCA
             3131  TGCGCA              
KpnI         3634  GGTACC               GGTAC^C
             3991  GGTACC              
SexAI         233  ACCTGGT              A^CC[AT]GGT
             4804  ACCTGGT             
SphI         1184  GCATGC               GCATG^C
             2099  GCATGC              
TfiI         1605  GATTC                G^A[AT]TC
             4050  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  164 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       ApoI        Asp700I     
AspEI       AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcgI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SacII       SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        SspBI       StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        XmnI