################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AccIII AclI AflII AscI AseI AsnI AvrII BclI BfrI BlnI BsaI BseAI BspEI BspHI BspLU11I Bst1107I BstXI BstZ17I CpoI DrdI EcoRV EcoT22I FbaI HincII HindII HpaI MfeI MluI MroI MunI NdeI NheI NruI NsiI PacI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AflIII 1667 ACGTGT A^C[GA][CT]GT AgeI 1824 ACCGGT A^CCGGT BbrPI 2344 CACGTG CAC^GTG BciVI 3531 GGATAC GTATCC(6/5) BsaBI 4740 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 2778 CGTACG C^GTACG BspDI 5334 ATCGAT AT^CGAT BssHII 5592 GCGCGC G^CGCGC BstBI 3301 TTCGAA TT^CGAA ClaI 5334 ATCGAT AT^CGAT DraI 6336 TTTAAA TTT^AAA FseI 1588 GGCCGGCC GGCCGG^CC HindIII 5446 AAGCTT A^AGCTT MamI 4740 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NotI 4879 GCGGCCGC GC^GGCCGC NspV 3301 TTCGAA TT^CGAA PaeR7I 4912 CTCGAG C^TCGAG PinAI 1824 ACCGGT A^CCGGT PmaCI 2344 CACGTG CAC^GTG PmlI 2344 CACGTG CAC^GTG SfuI 3301 TTCGAA TT^CGAA SgrAI 1823 CACCGGTG C[GA]^CCGG[CT]G XhoI 4912 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AatII 3857 GACGTC GACGT^C 5401 GACGTC Acc65I 3634 GGTACC G^GTACC 3991 GGTACC AccI 1831 GTCTAC GT^[AC][GT]AC 5128 GTCTAC Aor51HI 1231 AGCGCT AGC^GCT 3821 AGCGCT Asp718I 3634 GGTACC G^GTACC 3991 GGTACC AviII 1712 TGCGCA TGC^GCA 3131 TGCGCA BamHI 305 GGATCC G^GATCC 1985 GGATCC BglII 884 AGATCT A^GATCT 5354 AGATCT BsmBI 491 GAGACG CGTCTC(1/5) 5698 GAGACG BsmI 283 GAATGC GAATGC(1/-1) 3394 GCATTC BsrBI 978 CCGCTC CCGCTC(-3/-3) 634 GAGCGG DraIII 2341 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 3169 CACTACGTG Eco47III 1231 AGCGCT AGC^GCT 3821 AGCGCT EcoRI 241 GAATTC G^AATTC 3139 GAATTC FspI 1712 TGCGCA TGC^GCA 3131 TGCGCA KpnI 3634 GGTACC GGTAC^C 3991 GGTACC SexAI 233 ACCTGGT A^CC[AT]GGT 4804 ACCTGGT SphI 1184 GCATGC GCATG^C 2099 GCATGC TfiI 1605 GATTC G^A[AT]TC 4050 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 164 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AhdI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp700I AspEI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspBI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI