################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AscI AseI AsnI Asp718I AvrII BamHI BbeI BcgI BciVI BfrI BglII BlnI BsaBI BsiEI BsiWI BspDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 308 CGCG CG^CG AccIII 1386 TCCGGA T^CCGGA AcyI 559 GACGCC G[GA]^CG[CT]C AhdI 225 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1856 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 225 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1231 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1027 TGCGCA TGC^GCA BbrPI 2397 CACGTG CAC^GTG BclI 2684 TGATCA T^GATCA BlpI 199 GCTGAGC GC^T[ACGT]AGC Bpu1102I 199 GCTGAGC GC^T[ACGT]AGC BsaAI 2397 CACGTG [CT]AC^GT[GA] BsaHI 559 GACGCC G[GA]^CG[CT]C BseAI 1386 TCCGGA T^CCGGA BsmI 731 GCATTC GAATGC(1/-1) BspEI 1386 TCCGGA T^CCGGA BspHI 3976 TCATGA T^CATGA BspLU11I 2774 ACATGT A^CATGT BsrBI 3588 CCGCTC CCGCTC(-3/-3) BsrFI 382 ACCGGC [GA]^CCGG[CT] BssSI 1716 CTCGTG CACGAG(-5/-1) BstUI 308 CGCG CG^CG CelII 199 GCTGAGC GC^T[ACGT]AGC Cfr10I 382 ACCGGC [GA]^CCGG[CT] DraI 207 TTTAAA TTT^AAA DraIII 698 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 225 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 2684 TGATCA T^GATCA FspI 1027 TGCGCA TGC^GCA Hin1I 559 GACGCC G[GA]^CG[CT]C HincII 3273 GTTAAC GT[CT]^[GA]AC HindII 3273 GTTAAC GT[CT]^[GA]AC HpaI 3273 GTTAAC GTT^AAC MroI 1386 TCCGGA T^CCGGA MvnI 308 CGCG CG^CG PflFI 1231 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2397 CACGTG CAC^GTG PmlI 2397 CACGTG CAC^GTG RcaI 3976 TCATGA T^CATGA Tth111I 1231 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1856 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 976 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 988 CAGCCTCTG AocI 1760 CCTCAGG CC^T[ACGT]AGG 4286 CCTTAGG Aor51HI 2317 AGCGCT AGC^GCT 2368 AGCGCT ApaI 959 GGGCCC GGGCC^C 3352 GGGCCC BalI 1793 TGGCCA TGG^CCA 4183 TGGCCA BglI 449 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1035 GCCTCCGAGGC BsmBI 1130 GAGACG CGTCTC(1/5) 2665 GAGACG BsrDI 2645 GCAATG GCAATG(2/0) 3877 GCAATG Bsu36I 1760 CCTCAGG CC^T[ACGT]AGG 4286 CCTTAGG EaeI 1793 TGGCCA [CT]^GGCC[GA] 4183 TGGCCA Eco47III 2317 AGCGCT AGC^GCT 2368 AGCGCT Eco81I 1760 CCTCAGG CC^T[ACGT]AGG 4286 CCTTAGG EcoNI 1161 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1934 CCTTTATGAGG FauI 1435 CCCGC CCCGC(4/6) 4385 CCCGC HindIII 1662 AAGCTT A^AGCTT 3739 AAGCTT MluNI 1793 TGGCCA TGG^CCA 4183 TGGCCA MscI 1793 TGGCCA TGG^CCA 4183 TGGCCA PaeR7I 517 CTCGAG C^TCGAG 934 CTCGAG SacI 1052 GAGCTC GAGCT^C 4161 GAGCTC XhoI 517 CTCGAG C^TCGAG 934 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI