##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AscI        AseI        AsnI        Asp718I     
AvrII       BamHI       BbeI        BcgI        BciVI       
BfrI        BglII       BlnI        BsaBI       BsiEI       
BsiWI       BspDI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco52I      EcoO65I     EcoRV       
EcoT22I     FseI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         308  CGCG                 CG^CG
AccIII       1386  TCCGGA               T^CCGGA
AcyI          559  GACGCC               G[GA]^CG[CT]C
AhdI          225  GACTTAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1856  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         225  GACTTAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1231  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1027  TGCGCA               TGC^GCA
BbrPI        2397  CACGTG               CAC^GTG
BclI         2684  TGATCA               T^GATCA
BlpI          199  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      199  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2397  CACGTG               [CT]AC^GT[GA]
BsaHI         559  GACGCC               G[GA]^CG[CT]C
BseAI        1386  TCCGGA               T^CCGGA
BsmI          731  GCATTC               GAATGC(1/-1)
BspEI        1386  TCCGGA               T^CCGGA
BspHI        3976  TCATGA               T^CATGA
BspLU11I     2774  ACATGT               A^CATGT
BsrBI        3588  CCGCTC               CCGCTC(-3/-3)
BsrFI         382  ACCGGC               [GA]^CCGG[CT]
BssSI        1716  CTCGTG               CACGAG(-5/-1)
BstUI         308  CGCG                 CG^CG
CelII         199  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        382  ACCGGC               [GA]^CCGG[CT]
DraI          207  TTTAAA               TTT^AAA
DraIII        698  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      225  GACTTAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         2684  TGATCA               T^GATCA
FspI         1027  TGCGCA               TGC^GCA
Hin1I         559  GACGCC               G[GA]^CG[CT]C
HincII       3273  GTTAAC               GT[CT]^[GA]AC
HindII       3273  GTTAAC               GT[CT]^[GA]AC
HpaI         3273  GTTAAC               GTT^AAC
MroI         1386  TCCGGA               T^CCGGA
MvnI          308  CGCG                 CG^CG
PflFI        1231  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2397  CACGTG               CAC^GTG
PmlI         2397  CACGTG               CAC^GTG
RcaI         3976  TCATGA               T^CATGA
Tth111I      1231  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1856  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         976  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              988  CAGCCTCTG           
AocI         1760  CCTCAGG              CC^T[ACGT]AGG
             4286  CCTTAGG             
Aor51HI      2317  AGCGCT               AGC^GCT
             2368  AGCGCT              
ApaI          959  GGGCCC               GGGCC^C
             3352  GGGCCC              
BalI         1793  TGGCCA               TGG^CCA
             4183  TGGCCA              
BglI          449  GCCTGACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1035  GCCTCCGAGGC         
BsmBI        1130  GAGACG               CGTCTC(1/5)
             2665  GAGACG              
BsrDI        2645  GCAATG               GCAATG(2/0)
             3877  GCAATG              
Bsu36I       1760  CCTCAGG              CC^T[ACGT]AGG
             4286  CCTTAGG             
EaeI         1793  TGGCCA               [CT]^GGCC[GA]
             4183  TGGCCA              
Eco47III     2317  AGCGCT               AGC^GCT
             2368  AGCGCT              
Eco81I       1760  CCTCAGG              CC^T[ACGT]AGG
             4286  CCTTAGG             
EcoNI        1161  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1934  CCTTTATGAGG         
FauI         1435  CCCGC                CCCGC(4/6)
             4385  CCCGC               
HindIII      1662  AAGCTT               A^AGCTT
             3739  AAGCTT              
MluNI        1793  TGGCCA               TGG^CCA
             4183  TGGCCA              
MscI         1793  TGGCCA               TGG^CCA
             4183  TGGCCA              
PaeR7I        517  CTCGAG               C^TCGAG
              934  CTCGAG              
SacI         1052  GAGCTC               GAGCT^C
             4161  GAGCTC              
XhoI          517  CTCGAG               C^TCGAG
              934  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        ApaLI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EarI        EcoO109I    EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        SphI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI