##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BclI BfrI BglII BlnI BsiWI
BsmBI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KpnI MfeI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SapI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 3161 GTGCAC G^TGCAC
Aor51HI 1060 AGCGCT AGC^GCT
ApaLI 3161 GTGCAC G^TGCAC
Asp700I 922 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 3660 GCTGAGG CCTCAGC(-5/-2)
BcgI 1335 CGAAGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 3124 CTAG C^TAG
BlpI 320 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 320 GCTCAGC GC^T[ACGT]AGC
BsmI 2544 GAATGC GAATGC(1/-1)
BspHI 435 TCATGA T^CATGA
BspLU11I 3866 ACATGT A^CATGT
BssHII 2765 GCGCGC G^CGCGC
CelII 320 GCTCAGC GC^T[ACGT]AGC
DrdI 1658 GACCGGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1640 CGGCCG C^GGCCG
EclXI 1640 CGGCCG C^GGCCG
Eco47III 1060 AGCGCT AGC^GCT
Eco52I 1640 CGGCCG C^GGCCG
HincII 3101 GTCAAC GT[CT]^[GA]AC
HindII 3101 GTCAAC GT[CT]^[GA]AC
HindIII 3536 AAGCTT A^AGCTT
KspI 1212 CCGCGG CCGC^GG
MaeI 3124 CTAG C^TAG
MluI 510 ACGCGT A^CGCGT
MslI 133 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 4512 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 435 TCATGA T^CATGA
SacII 1212 CCGCGG CCGC^GG
SbfI 605 CCTGCAGG CCTGCA^GG
SfiI 1081 GGCCTCGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 605 CCTGCAGG CCTGCA^GG
SspI 2976 AATATT AAT^ATT
Van91I 4512 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 922 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 3269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3751 GACCCTGTC
AviII 1228 TGCGCA TGC^GCA
3491 TGCGCA
BbeI 337 GGCGCC GGCGC^C
4360 GGCGCC
BbrPI 1346 CACGTG CAC^GTG
3834 CACGTG
BciVI 65 GGATAC GTATCC(6/5)
383 GGATAC
BsaAI 1346 CACGTG [CT]AC^GT[GA]
3834 CACGTG
BsaBI 2257 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4038 GATGAGGATC
BsiEI 1640 CGGCCG CG[GA][CT]^CG
1657 CGACCG
BsrDI 848 CATTGC GCAATG(2/0)
1405 CATTGC
BssSI 2702 CTCGTG CACGAG(-5/-1)
3450 CTCGTG
DraIII 3447 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
4140 CACTGAGTG
EarI 1802 GAAGAG CTCTTC(1/4)
2626 GAAGAG
FspI 1228 TGCGCA TGC^GCA
3491 TGCGCA
KasI 337 GGCGCC G^GCGCC
4360 GGCGCC
Ksp632I 1802 GAAGAG CTCTTC(1/4)
2626 GAAGAG
MamI 2257 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4038 GATGAGGATC
NarI 337 GGCGCC GG^CGCC
4360 GGCGCC
NspI 3866 ACATGT [GA]CATG^[CT]
4558 GCATGT
PflFI 3269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3751 GACCCTGTC
PmaCI 1346 CACGTG CAC^GTG
3834 CACGTG
PmlI 1346 CACGTG CAC^GTG
3834 CACGTG
SacI 1706 GAGCTC GAGCT^C
3889 GAGCTC
SfoI 337 GGCGCC GGC^GCC
4360 GGCGCC
TfiI 3022 GATTC G^A[AT]TC
3410 GATTC
Tth111I 3269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3751 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PaeR7I PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoI XhoII XmaCI XmaI