##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AgeI        AhdI        AocI        ApaI        AscI        
AspEI       AviII       AvrII       BamHI       BcgI        
BglII       BlnI        BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BsrDI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      FseI        FspI        HgaI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       Sse8387I    StuI        
SwaI        Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       1869  GGTACC               G^GTACC
AcyI         2381  GGCGCC               G[GA]^CG[CT]C
Alw44I       2031  GTGCAC               G^TGCAC
Aor51HI      2675  AGCGCT               AGC^GCT
ApaLI        2031  GTGCAC               G^TGCAC
AseI         4371  ATTAAT               AT^TAAT
AsnI         4371  ATTAAT               AT^TAAT
Asp718I      1869  GGTACC               G^GTACC
AspI         2896  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1015  CTCGGG               C^[CT]CG[GA]G
BbeI         2381  GGCGCC               GGCGC^C
BbrPI        2581  CACGTG               CAC^GTG
BbvCI        1864  GCTGAGG              CCTCAGC(-5/-2)
BclI         2358  TGATCA               T^GATCA
BcnI         2256  CCGGG                CC^[GC]GG
BsaAI        2581  CACGTG               [CT]AC^GT[GA]
BsaHI        2381  GGCGCC               G[GA]^CG[CT]C
BsaI         4172  GGTCTC               GGTCTC(1/5)
BsoBI        1015  CTCGGG               C^[CT]CG[GA]G
BspMI        2934  ACCTGC               ACCTGC(4/8)
BsrBI        3445  GAGCGG               CCGCTC(-3/-3)
BsrFI        3149  GCCGGC               [GA]^CCGG[CT]
BstEII       3027  GGTTACC              G^GT[ACGT]ACC
BstPI        3027  GGTTACC              G^GT[ACGT]ACC
Cfr10I       3149  GCCGGC               [GA]^CCGG[CT]
Eco47III     2675  AGCGCT               AGC^GCT
EcoO65I      3027  GGTTACC              G^GT[ACGT]ACC
EcoRV        4687  GATATC               GAT^ATC
FbaI         2358  TGATCA               T^GATCA
Hin1I        2381  GGCGCC               G[GA]^CG[CT]C
HpaI         3889  GTTAAC               GTT^AAC
KasI         2381  GGCGCC               G^GCGCC
KpnI         1869  GGTACC               GGTAC^C
MaeII        2582  ACGT                 A^CGT
NaeI         3149  GCCGGC               GCC^GGC
NarI         2381  GGCGCC               GG^CGCC
NciI         2256  CCGGG                CC^[GC]GG
NdeI          474  CATATG               CA^TATG
NgoMIV       3149  GCCGGC               G^CCGGC
NheI         2167  GCTAGC               G^CTAGC
PflFI        2896  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2581  CACGTG               CAC^GTG
PmlI         2581  CACGTG               CAC^GTG
PpuMI        2716  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        4995  GACAGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4371  ATTAAT               AT^TAAT
SapI         3532  GCTCTTC              GCTCTTC(1/4)
SfoI         2381  GGCGCC               GGC^GCC
SpeI         4600  ACTAGT               A^CTAGT
SphI           92  GCATGC               GCATG^C
Tth111I      2896  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2507  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AflII        1653  CTTAAG               C^TTAAG
             4260  CTTAAG              
BalI         1558  TGGCCA               TGG^CCA
             1672  TGGCCA              
BciVI         276  GTATCC               GTATCC(6/5)
             4928  GTATCC              
BfrI         1653  CTTAAG               C^TTAAG
             4260  CTTAAG              
BglI         2109  GCCACCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2147  GCCATTCGGGC         
FauI         1419  GCGGG                CCCGC(4/6)
             4456  GCGGG               
HaeII        2381  GGCGCC               [GA]GCGC^[CT]
             2675  AGCGCT              
MluNI        1558  TGGCCA               TGG^CCA
             1672  TGGCCA              
MscI         1558  TGGCCA               TGG^CCA
             1672  TGGCCA              
SacI         2682  GAGCTC               GAGCT^C
             2788  GAGCTC              
XcmI           23  CCATTCTCAGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2110  CCACCAAGGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BanI        BanII       BbsI        BbvI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspHI       BspLU11I    BsrGI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRI       EcoRII      EcoT14I     EcoT22I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI