################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccI AccII AccIII AclI AgeI AhdI AocI ApaI AscI AspEI AviII AvrII BamHI BcgI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrDI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I FseI FspI HgaI KspI MamI MfeI MluI MroI MunI MvnI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1869 GGTACC G^GTACC AcyI 2381 GGCGCC G[GA]^CG[CT]C Alw44I 2031 GTGCAC G^TGCAC Aor51HI 2675 AGCGCT AGC^GCT ApaLI 2031 GTGCAC G^TGCAC AseI 4371 ATTAAT AT^TAAT AsnI 4371 ATTAAT AT^TAAT Asp718I 1869 GGTACC G^GTACC AspI 2896 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1015 CTCGGG C^[CT]CG[GA]G BbeI 2381 GGCGCC GGCGC^C BbrPI 2581 CACGTG CAC^GTG BbvCI 1864 GCTGAGG CCTCAGC(-5/-2) BclI 2358 TGATCA T^GATCA BcnI 2256 CCGGG CC^[GC]GG BsaAI 2581 CACGTG [CT]AC^GT[GA] BsaHI 2381 GGCGCC G[GA]^CG[CT]C BsaI 4172 GGTCTC GGTCTC(1/5) BsoBI 1015 CTCGGG C^[CT]CG[GA]G BspMI 2934 ACCTGC ACCTGC(4/8) BsrBI 3445 GAGCGG CCGCTC(-3/-3) BsrFI 3149 GCCGGC [GA]^CCGG[CT] BstEII 3027 GGTTACC G^GT[ACGT]ACC BstPI 3027 GGTTACC G^GT[ACGT]ACC Cfr10I 3149 GCCGGC [GA]^CCGG[CT] Eco47III 2675 AGCGCT AGC^GCT EcoO65I 3027 GGTTACC G^GT[ACGT]ACC EcoRV 4687 GATATC GAT^ATC FbaI 2358 TGATCA T^GATCA Hin1I 2381 GGCGCC G[GA]^CG[CT]C HpaI 3889 GTTAAC GTT^AAC KasI 2381 GGCGCC G^GCGCC KpnI 1869 GGTACC GGTAC^C MaeII 2582 ACGT A^CGT NaeI 3149 GCCGGC GCC^GGC NarI 2381 GGCGCC GG^CGCC NciI 2256 CCGGG CC^[GC]GG NdeI 474 CATATG CA^TATG NgoMIV 3149 GCCGGC G^CCGGC NheI 2167 GCTAGC G^CTAGC PflFI 2896 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2581 CACGTG CAC^GTG PmlI 2581 CACGTG CAC^GTG PpuMI 2716 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 4995 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4371 ATTAAT AT^TAAT SapI 3532 GCTCTTC GCTCTTC(1/4) SfoI 2381 GGCGCC GGC^GCC SpeI 4600 ACTAGT A^CTAGT SphI 92 GCATGC GCATG^C Tth111I 2896 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2507 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AflII 1653 CTTAAG C^TTAAG 4260 CTTAAG BalI 1558 TGGCCA TGG^CCA 1672 TGGCCA BciVI 276 GTATCC GTATCC(6/5) 4928 GTATCC BfrI 1653 CTTAAG C^TTAAG 4260 CTTAAG BglI 2109 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2147 GCCATTCGGGC FauI 1419 GCGGG CCCGC(4/6) 4456 GCGGG HaeII 2381 GGCGCC [GA]GCGC^[CT] 2675 AGCGCT MluNI 1558 TGGCCA TGG^CCA 1672 TGGCCA MscI 1558 TGGCCA TGG^CCA 1672 TGGCCA SacI 2682 GAGCTC GAGCT^C 2788 GAGCTC XcmI 23 CCATTCTCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2110 CCACCAAGGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspLU11I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI