##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI Asp700I Asp718I AspI BbrPI
BclI BfrI BglII BsaBI BsgI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraIII EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HpaI KpnI MamI MfeI
MluI MunI NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1487 GACGTC GACGT^C
AflIII 3417 ACGTGT A^C[GA][CT]GT
AhdI 1402 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1685 AGCGCT AGC^GCT
AseI 4254 ATTAAT AT^TAAT
AsnI 4254 ATTAAT AT^TAAT
AspEI 1402 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1523 TGCGCA TGC^GCA
AvrII 3941 CCTAGG C^CTAGG
BalI 2015 TGGCCA TGG^CCA
BamHI 2158 GGATCC G^GATCC
BlnI 3941 CCTAGG C^CTAGG
BsaAI 835 TACGTG [CT]AC^GT[GA]
BsmBI 2369 GAGACG CGTCTC(1/5)
BsrBI 1478 GAGCGG CCGCTC(-3/-3)
BsrDI 3004 CATTGC GCAATG(2/0)
BssHII 1650 GCGCGC G^CGCGC
BstXI 2603 CCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1557 CGGACCG CG^G[AT]CCG
DraI 179 TTTAAA TTT^AAA
Eam1105I 1402 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1685 AGCGCT AGC^GCT
EcoT22I 789 ATGCAT ATGCA^T
FspI 1523 TGCGCA TGC^GCA
HindIII 2270 AAGCTT A^AGCTT
KspI 1041 CCGCGG CCGC^GG
MluNI 2015 TGGCCA TGG^CCA
MscI 2015 TGGCCA TGG^CCA
NaeI 1435 GCCGGC GCC^GGC
NcoI 576 CCATGG C^CATGG
NdeI 2939 CATATG CA^TATG
NgoMIV 1435 GCCGGC G^CCGGC
NsiI 789 ATGCAT ATGCA^T
PaeR7I 2520 CTCGAG C^TCGAG
PflMI 2267 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2245 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4254 ATTAAT AT^TAAT
PvuII 456 CAGCTG CAG^CTG
RsrII 1557 CGGACCG CG^G[AT]CCG
SacII 1041 CCGCGG CCGC^GG
SphI 3398 GCATGC GCATG^C
Van91I 2267 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2926 TCTAGA T^CTAGA
XhoI 2520 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 478 TCCGGA T^CCGGA
520 TCCGGA
Alw44I 879 GTGCAC G^TGCAC
1804 GTGCAC
ApaLI 879 GTGCAC G^TGCAC
1804 GTGCAC
BbeI 892 GGCGCC GGCGC^C
1888 GGCGCC
BcgI 2577 CGAGAGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1664 GCAGCGGCTTCG
BciVI 132 GTATCC GTATCC(6/5)
568 GTATCC
BsaI 1153 GAGACC GGTCTC(1/5)
5 GGTCTC
BseAI 478 TCCGGA T^CCGGA
520 TCCGGA
BsiEI 1433 CGGCCG CG[GA][CT]^CG
1589 CGGCCG
BsmI 4051 GAATGC GAATGC(1/-1)
2650 GCATTC
BspEI 478 TCCGGA T^CCGGA
520 TCCGGA
BspMI 2036 ACCTGC ACCTGC(4/8)
2733 ACCTGC
DrdI 1873 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3954 GACTGCAGGGTC
EagI 1433 CGGCCG C^GGCCG
1589 CGGCCG
EclXI 1433 CGGCCG C^GGCCG
1589 CGGCCG
Eco52I 1433 CGGCCG C^GGCCG
1589 CGGCCG
KasI 892 GGCGCC G^GCGCC
1888 GGCGCC
MroI 478 TCCGGA T^CCGGA
520 TCCGGA
MslI 1626 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2490 CATCGCCATG
NarI 892 GGCGCC GG^CGCC
1888 GGCGCC
SacI 2554 GAGCTC GAGCT^C
3688 GAGCTC
SfoI 892 GGCGCC GGC^GCC
1888 GGCGCC
XcmI 2489 CCATCGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2603 CCAACCCCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI