################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## Acc65I AclI Aor51HI AscI Asp718I AviII AvrII BbeI BbrPI BlnI BsaBI BsiWI BspDI BssHII Bst1107I BstZ17I ClaI CpoI DrdI Eco47III EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NheI NruI NsiI PacI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI ScaI SfoI SgrAI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 826 GACGTC GACGT^C AccIII 5465 TCCGGA T^CCGGA AflII 2698 CTTAAG C^TTAAG AgeI 6542 ACCGGT A^CCGGT Alw44I 1734 GTGCAC G^TGCAC AocI 387 CCTGAGG CC^T[ACGT]AGG ApaLI 1734 GTGCAC G^TGCAC AseI 1234 ATTAAT AT^TAAT AsnI 1234 ATTAAT AT^TAAT BamHI 1442 GGATCC G^GATCC BciVI 1379 GGATAC GTATCC(6/5) BfrI 2698 CTTAAG C^TTAAG BsaAI 420 TACGTA [CT]AC^GT[GA] BseAI 5465 TCCGGA T^CCGGA BspEI 5465 TCCGGA T^CCGGA BspHI 2321 TCATGA T^CATGA BspLU11I 494 ACATGT A^CATGT BstBI 4801 TTCGAA TT^CGAA BstEII 6538 GGTAACC G^GT[ACGT]ACC BstPI 6538 GGTAACC G^GT[ACGT]ACC Bsu36I 387 CCTGAGG CC^T[ACGT]AGG DraI 6222 TTTAAA TTT^AAA DraIII 6717 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 4310 CGGCCG C^GGCCG EclXI 4310 CGGCCG C^GGCCG Eco52I 4310 CGGCCG C^GGCCG Eco81I 387 CCTGAGG CC^T[ACGT]AGG EcoO65I 6538 GGTAACC G^GT[ACGT]ACC EcoRI 6669 GAATTC G^AATTC MroI 5465 TCCGGA T^CCGGA NdeI 1358 CATATG CA^TATG NotI 4309 GCGGCCGC GC^GGCCGC NspV 4801 TTCGAA TT^CGAA PinAI 6542 ACCGGT A^CCGGT PshBI 1234 ATTAAT AT^TAAT RcaI 2321 TCATGA T^CATGA SalI 490 GTCGAC G^TCGAC SfiI 660 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 4801 TTCGAA TT^CGAA SnaBI 420 TACGTA TAC^GTA SpeI 35 ACTAGT A^CTAGT SphI 566 GCATGC GCATG^C XbaI 5890 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 490 GTCGAC GT^[AC][GT]AC 4077 GTCTAC AflIII 494 ACATGT A^C[GA][CT]GT 4073 ACGTGT AhdI 1177 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4000 GACGTGGCGTC AspEI 1177 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4000 GACGTGGCGTC BanI 70 GGCACC G^G[CT][GA]CC 6595 GGTGCC BcgI 6588 CGAAATGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 815 GCATCAACTTCG BlpI 5379 GCTCAGC GC^T[ACGT]AGC 6277 GCTGAGC Bpu1102I 5379 GCTCAGC GC^T[ACGT]AGC 6277 GCTGAGC Bsp1407I 497 TGTACA T^GTACA 2042 TGTACA BsrGI 497 TGTACA T^GTACA 2042 TGTACA CelII 5379 GCTCAGC GC^T[ACGT]AGC 6277 GCTGAGC Eam1105I 1177 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4000 GACGTGGCGTC NaeI 2207 GCCGGC GCC^GGC 6601 GCCGGC NgoMIV 2207 GCCGGC G^CCGGC 6601 GCCGGC SexAI 4367 ACCTGGT A^CC[AT]GGT 4619 ACCTGGT SmaI 3896 CCCGGG CCC^GGG 4820 CCCGGG SspBI 497 TGTACA T^GTACA 2042 TGTACA XmaCI 3896 CCCGGG C^CCGGG 4820 CCCGGG XmaI 3896 CCCGGG C^CCGGG 4820 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmnI