##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

Acc65I      AclI        Aor51HI     AscI        Asp718I     
AviII       AvrII       BbeI        BbrPI       BlnI        
BsaBI       BsiWI       BspDI       BssHII      Bst1107I    
BstZ17I     ClaI        CpoI        DrdI        Eco47III    
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NarI        NheI        
NruI        NsiI        PacI        PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SbfI        ScaI        SfoI        SgrAI       
Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         826  GACGTC               GACGT^C
AccIII       5465  TCCGGA               T^CCGGA
AflII        2698  CTTAAG               C^TTAAG
AgeI         6542  ACCGGT               A^CCGGT
Alw44I       1734  GTGCAC               G^TGCAC
AocI          387  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1734  GTGCAC               G^TGCAC
AseI         1234  ATTAAT               AT^TAAT
AsnI         1234  ATTAAT               AT^TAAT
BamHI        1442  GGATCC               G^GATCC
BciVI        1379  GGATAC               GTATCC(6/5)
BfrI         2698  CTTAAG               C^TTAAG
BsaAI         420  TACGTA               [CT]AC^GT[GA]
BseAI        5465  TCCGGA               T^CCGGA
BspEI        5465  TCCGGA               T^CCGGA
BspHI        2321  TCATGA               T^CATGA
BspLU11I      494  ACATGT               A^CATGT
BstBI        4801  TTCGAA               TT^CGAA
BstEII       6538  GGTAACC              G^GT[ACGT]ACC
BstPI        6538  GGTAACC              G^GT[ACGT]ACC
Bsu36I        387  CCTGAGG              CC^T[ACGT]AGG
DraI         6222  TTTAAA               TTT^AAA
DraIII       6717  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         4310  CGGCCG               C^GGCCG
EclXI        4310  CGGCCG               C^GGCCG
Eco52I       4310  CGGCCG               C^GGCCG
Eco81I        387  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      6538  GGTAACC              G^GT[ACGT]ACC
EcoRI        6669  GAATTC               G^AATTC
MroI         5465  TCCGGA               T^CCGGA
NdeI         1358  CATATG               CA^TATG
NotI         4309  GCGGCCGC             GC^GGCCGC
NspV         4801  TTCGAA               TT^CGAA
PinAI        6542  ACCGGT               A^CCGGT
PshBI        1234  ATTAAT               AT^TAAT
RcaI         2321  TCATGA               T^CATGA
SalI          490  GTCGAC               G^TCGAC
SfiI          660  GGCCGTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         4801  TTCGAA               TT^CGAA
SnaBI         420  TACGTA               TAC^GTA
SpeI           35  ACTAGT               A^CTAGT
SphI          566  GCATGC               GCATG^C
XbaI         5890  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          490  GTCGAC               GT^[AC][GT]AC
             4077  GTCTAC              
AflIII        494  ACATGT               A^C[GA][CT]GT
             4073  ACGTGT              
AhdI         1177  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4000  GACGTGGCGTC         
AspEI        1177  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4000  GACGTGGCGTC         
BanI           70  GGCACC               G^G[CT][GA]CC
             6595  GGTGCC              
BcgI         6588  CGAAATGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              815  GCATCAACTTCG        
BlpI         5379  GCTCAGC              GC^T[ACGT]AGC
             6277  GCTGAGC             
Bpu1102I     5379  GCTCAGC              GC^T[ACGT]AGC
             6277  GCTGAGC             
Bsp1407I      497  TGTACA               T^GTACA
             2042  TGTACA              
BsrGI         497  TGTACA               T^GTACA
             2042  TGTACA              
CelII        5379  GCTCAGC              GC^T[ACGT]AGC
             6277  GCTGAGC             
Eam1105I     1177  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4000  GACGTGGCGTC         
NaeI         2207  GCCGGC               GCC^GGC
             6601  GCCGGC              
NgoMIV       2207  GCCGGC               G^CCGGC
             6601  GCCGGC              
SexAI        4367  ACCTGGT              A^CC[AT]GGT
             4619  ACCTGGT             
SmaI         3896  CCCGGG               CCC^GGG
             4820  CCCGGG              
SspBI         497  TGTACA               T^GTACA
             2042  TGTACA              
XmaCI        3896  CCCGGG               C^CCGGG
             4820  CCCGGG              
XmaI         3896  CCCGGG               C^CCGGG
             4820  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AspI        AvaI        AvaII       
BalI        BanII       BbsI        BbvCI       BbvI        
BclI        BcnI        BfaI        BglI        BglII       
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PflFI       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoI        XhoII       XmnI