##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BamHI BbrPI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI FspI KpnI KspI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I StuI
SwaI Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 3532 GTCTAC GT^[AC][GT]AC
AlwI 1280 GATCC GGATC(4/5)
AlwNI 129 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 178 GGGCCC GGGCC^C
AseI 2495 ATTAAT AT^TAAT
AsnI 2495 ATTAAT AT^TAAT
AspI 1983 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 282 GGCGCC GGCGC^C
BcgI 1519 CGAAGGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 980 GGATAC GTATCC(6/5)
BclI 1333 TGATCA T^GATCA
BglII 1666 AGATCT A^GATCT
BpmI 2881 CTCCAG CTGGAG(16/14)
BsaBI 1295 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 3604 GAGGAG GAGGAG(10/8)
BsiEI 222 CGGTCG CG[GA][CT]^CG
BspDI 1277 ATCGAT AT^CGAT
BspHI 1990 TCATGA T^CATGA
BsrI 360 CCAGT ACTGG(1/-1)
BssSI 3027 CTCGTG CACGAG(-5/-1)
BstAPI 1234 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2516 CCACTCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1666 AGATCT [GA]^GATC[CT]
ClaI 1277 ATCGAT AT^CGAT
DraII 391 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 1740 CCATGG C^C[GA][CT]GG
EcoO109I 391 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 890 GATATC GAT^ATC
FbaI 1333 TGATCA T^GATCA
HgaI 308 GACGC GACGC(5/10)
HpaI 2358 GTTAAC GTT^AAC
KasI 282 GGCGCC G^GCGCC
MamI 1295 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1666 AGATCT [GA]^GATC[CT]
NarI 282 GGCGCC GG^CGCC
NcoI 1740 CCATGG C^CATGG
NdeI 1626 CATATG CA^TATG
NruI 53 TCGCGA TCG^CGA
PaeR7I 2831 CTCGAG C^TCGAG
PflFI 1983 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 391 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2495 ATTAAT AT^TAAT
PstI 2467 CTGCAG CTGCA^G
RcaI 1990 TCATGA T^CATGA
ScaI 2714 AGTACT AGT^ACT
SfiI 358 GGCCAGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 282 GGCGCC GGC^GCC
Tth111I 1983 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2831 CTCGAG C^TCGAG
XhoII 1666 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 282 GGCGCC G[GA]^CG[CT]C
308 GACGCC
AflII 3135 CTTAAG C^TTAAG
3494 CTTAAG
BalI 1812 TGGCCA TGG^CCA
2191 TGGCCA
BbvCI 2803 GCTGAGG CCTCAGC(-5/-2)
3169 GCTGAGG
BfrI 3135 CTTAAG C^TTAAG
3494 CTTAAG
BglI 199 GCCGCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
359 GCCAGTCCGGC
BsaHI 282 GGCGCC G[GA]^CG[CT]C
308 GACGCC
BsmFI 170 GGGAC GGGAC(10/14)
393 GTCCC
BsmI 1037 GAATGC GAATGC(1/-1)
1288 GAATGC
BspMI 1107 ACCTGC ACCTGC(4/8)
2861 ACCTGC
BsrDI 1083 CATTGC GCAATG(2/0)
1119 GCAATG
HaeII 48 GGCGCT [GA]GCGC^[CT]
282 GGCGCC
Hin1I 282 GGCGCC G[GA]^CG[CT]C
308 GACGCC
HincII 539 GTTGAC GT[CT]^[GA]AC
2358 GTTAAC
HindII 539 GTTGAC GT[CT]^[GA]AC
2358 GTTAAC
HindIII 1181 AAGCTT A^AGCTT
3268 AAGCTT
MaeII 667 ACGT A^CGT
2324 ACGT
MluNI 1812 TGGCCA TGG^CCA
2191 TGGCCA
MscI 1812 TGGCCA TGG^CCA
2191 TGGCCA
PshAI 82 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1383 GACCTGAGTC
PvuII 209 CAGCTG CAG^CTG
1316 CAGCTG
SacI 142 GAGCTC GAGCT^C
2828 GAGCTC
SfcI 525 CTACAG C^T[GA][CT]AG
2467 CTGCAG
SmaI 181 CCCGGG CCC^GGG
370 CCCGGG
SphI 762 GCATGC GCATG^C
2446 GCATGC
XcmI 993 CCAAACAGTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1815 CCAAATCGGAAATGG
XmaCI 181 CCCGGG C^CCGGG
370 CCCGGG
XmaI 181 CCCGGG C^CCGGG
370 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I Bsp1407I BspLU11I BsrGI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I