##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AgeI        Alw44I      ApaI        ApaLI       AscI        
Asp718I     AspI        AviII       AvrII       BbeI        
BbrPI       BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsiEI       BsiWI       
BspDI       BsrBI       BsrFI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DrdI        EagI        EclXI       
Eco52I      EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SacII       SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII       2040  TCCGGA               T^CCGGA
AflIII        520  ACATGT               A^C[GA][CT]GT
AocI         1655  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1309  AGCGCT               AGC^GCT
AseI         1291  ATTAAT               AT^TAAT
AsnI         1291  ATTAAT               AT^TAAT
AvaI         3678  CCCGAG               C^[CT]CG[GA]G
BanII        1026  GAGCTC               G[GA]GC[CT]^C
BbvCI         838  GCTGAGG              CCTCAGC(-5/-2)
BcnI         2189  CCCGG                CC^[GC]GG
BsaBI          65  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2715  GAGACC               GGTCTC(1/5)
BseAI        2040  TCCGGA               T^CCGGA
BsgI         2199  GTGCAG               GTGCAG(16/14)
BsoBI        3678  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1318  TGTACA               T^GTACA
BspEI        2040  TCCGGA               T^CCGGA
BspHI        2555  TCATGA               T^CATGA
BspLU11I      520  ACATGT               A^CATGT
BsrGI        1318  TGTACA               T^GTACA
BssSI        2252  CTCGTG               CACGAG(-5/-1)
BstEII       3089  GGTCACC              G^GT[ACGT]ACC
BstPI        3089  GGTCACC              G^GT[ACGT]ACC
BstXI        2296  CCACTTTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1655  CCTGAGG              CC^T[ACGT]AGG
CfoI         1310  GCGC                 GCG^C
DraIII         19  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1309  AGCGCT               AGC^GCT
Eco81I       1655  CCTGAGG              CC^T[ACGT]AGG
EcoNI        3652  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3089  GGTCACC              G^GT[ACGT]ACC
EcoRV        1328  GATATC               GAT^ATC
FauI         3700  CCCGC                CCCGC(4/6)
HaeII        1309  AGCGCT               [GA]GCGC^[CT]
HhaI         1310  GCGC                 GCG^C
HinP1I       1310  GCGC                 G^CGC
HincII       2031  GTCGAC               GT[CT]^[GA]AC
HindII       2031  GTCGAC               GT[CT]^[GA]AC
MamI           65  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2040  TCCGGA               T^CCGGA
NciI         2189  CCCGG                CC^[GC]GG
NdeI         1322  CATATG               CA^TATG
PshBI        1291  ATTAAT               AT^TAAT
RcaI         2555  TCATGA               T^CATGA
SacI         1026  GAGCTC               GAGCT^C
SalI         2031  GTCGAC               G^TCGAC
SphI         4094  GCATGC               GCATG^C
SspBI        1318  TGTACA               T^GTACA
SspI           88  AATATT               AAT^ATT
XbaI          200  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI         3151  GACGCC               G[GA]^CG[CT]C
             3834  GGCGTC              
AhdI         1974  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3804  GACTCCCAGTC         
AspEI        1974  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3804  GACTCCCAGTC         
AvaII        1924  GGTCC                G^G[AT]CC
             1981  GGTCC               
BanI         1090  GGTGCC               G^G[CT][GA]CC
             2601  GGTGCC              
BbsI         2371  GTCTTC               GAAGAC(2/6)
             3812  GTCTTC              
BclI         1918  TGATCA               T^GATCA
             3452  TGATCA              
BpuAI        2371  GTCTTC               GAAGAC(2/6)
             3812  GTCTTC              
BsaAI         440  TACGTG               [CT]AC^GT[GA]
             2130  TACGTG              
BsaHI        3151  GACGCC               G[GA]^CG[CT]C
             3834  GGCGTC              
BsaWI         990  ACCGGA               [AT]^CCGG[AT]
             2040  TCCGGA              
BstAPI       1550  GCAGGTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2895  GCATTTATTGC         
DraI         1745  TTTAAA               TTT^AAA
             2879  TTTAAA              
DsaI          306  CCATGG               C^C[GA][CT]GG
             3644  CCATGG              
Eam1105I     1974  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3804  GACTCCCAGTC         
EarI         2024  GAAGAG               CTCTTC(1/4)
             3745  GAAGAG              
FbaI         1918  TGATCA               T^GATCA
             3452  TGATCA              
Hin1I        3151  GACGCC               G[GA]^CG[CT]C
             3834  GGCGTC              
Ksp632I      2024  GAAGAG               CTCTTC(1/4)
             3745  GAAGAG              
NcoI          306  CCATGG               C^CATGG
             3644  CCATGG              
PpuMI        1923  AGGTCCT              [GA]G^G[AT]CC[CT]
             1980  AGGTCCT             
SexAI        1608  ACCAGGT              A^CC[AT]GGT
             2341  ACCAGGT             
SmlI         1205  CTTGAG               C^T[CT][GA]AG
             2502  CTTGAG              
TaqI         1024  TCGA                 T^CGA
             2032  TCGA                
TthHB8I      1024  TCGA                 T^CGA
             2032  TCGA                
XcmI           21  CCATGTGGGATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              404  CCACTGCTGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
BalI        BamHI       BbvI        BfaI        BglII       
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XhoII       XmnI