##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI Alw44I ApaI ApaLI AscI
Asp718I AspI AviII AvrII BbeI
BbrPI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsiEI BsiWI
BspDI BsrBI BsrFI BssHII Bst1107I
BstBI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 2040 TCCGGA T^CCGGA
AflIII 520 ACATGT A^C[GA][CT]GT
AocI 1655 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1309 AGCGCT AGC^GCT
AseI 1291 ATTAAT AT^TAAT
AsnI 1291 ATTAAT AT^TAAT
AvaI 3678 CCCGAG C^[CT]CG[GA]G
BanII 1026 GAGCTC G[GA]GC[CT]^C
BbvCI 838 GCTGAGG CCTCAGC(-5/-2)
BcnI 2189 CCCGG CC^[GC]GG
BsaBI 65 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2715 GAGACC GGTCTC(1/5)
BseAI 2040 TCCGGA T^CCGGA
BsgI 2199 GTGCAG GTGCAG(16/14)
BsoBI 3678 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1318 TGTACA T^GTACA
BspEI 2040 TCCGGA T^CCGGA
BspHI 2555 TCATGA T^CATGA
BspLU11I 520 ACATGT A^CATGT
BsrGI 1318 TGTACA T^GTACA
BssSI 2252 CTCGTG CACGAG(-5/-1)
BstEII 3089 GGTCACC G^GT[ACGT]ACC
BstPI 3089 GGTCACC G^GT[ACGT]ACC
BstXI 2296 CCACTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1655 CCTGAGG CC^T[ACGT]AGG
CfoI 1310 GCGC GCG^C
DraIII 19 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1309 AGCGCT AGC^GCT
Eco81I 1655 CCTGAGG CC^T[ACGT]AGG
EcoNI 3652 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3089 GGTCACC G^GT[ACGT]ACC
EcoRV 1328 GATATC GAT^ATC
FauI 3700 CCCGC CCCGC(4/6)
HaeII 1309 AGCGCT [GA]GCGC^[CT]
HhaI 1310 GCGC GCG^C
HinP1I 1310 GCGC G^CGC
HincII 2031 GTCGAC GT[CT]^[GA]AC
HindII 2031 GTCGAC GT[CT]^[GA]AC
MamI 65 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2040 TCCGGA T^CCGGA
NciI 2189 CCCGG CC^[GC]GG
NdeI 1322 CATATG CA^TATG
PshBI 1291 ATTAAT AT^TAAT
RcaI 2555 TCATGA T^CATGA
SacI 1026 GAGCTC GAGCT^C
SalI 2031 GTCGAC G^TCGAC
SphI 4094 GCATGC GCATG^C
SspBI 1318 TGTACA T^GTACA
SspI 88 AATATT AAT^ATT
XbaI 200 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 3151 GACGCC G[GA]^CG[CT]C
3834 GGCGTC
AhdI 1974 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3804 GACTCCCAGTC
AspEI 1974 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3804 GACTCCCAGTC
AvaII 1924 GGTCC G^G[AT]CC
1981 GGTCC
BanI 1090 GGTGCC G^G[CT][GA]CC
2601 GGTGCC
BbsI 2371 GTCTTC GAAGAC(2/6)
3812 GTCTTC
BclI 1918 TGATCA T^GATCA
3452 TGATCA
BpuAI 2371 GTCTTC GAAGAC(2/6)
3812 GTCTTC
BsaAI 440 TACGTG [CT]AC^GT[GA]
2130 TACGTG
BsaHI 3151 GACGCC G[GA]^CG[CT]C
3834 GGCGTC
BsaWI 990 ACCGGA [AT]^CCGG[AT]
2040 TCCGGA
BstAPI 1550 GCAGGTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2895 GCATTTATTGC
DraI 1745 TTTAAA TTT^AAA
2879 TTTAAA
DsaI 306 CCATGG C^C[GA][CT]GG
3644 CCATGG
Eam1105I 1974 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3804 GACTCCCAGTC
EarI 2024 GAAGAG CTCTTC(1/4)
3745 GAAGAG
FbaI 1918 TGATCA T^GATCA
3452 TGATCA
Hin1I 3151 GACGCC G[GA]^CG[CT]C
3834 GGCGTC
Ksp632I 2024 GAAGAG CTCTTC(1/4)
3745 GAAGAG
NcoI 306 CCATGG C^CATGG
3644 CCATGG
PpuMI 1923 AGGTCCT [GA]G^G[AT]CC[CT]
1980 AGGTCCT
SexAI 1608 ACCAGGT A^CC[AT]GGT
2341 ACCAGGT
SmlI 1205 CTTGAG C^T[CT][GA]AG
2502 CTTGAG
TaqI 1024 TCGA T^CGA
2032 TCGA
TthHB8I 1024 TCGA T^CGA
2032 TCGA
XcmI 21 CCATGTGGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
404 CCACTGCTGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
BalI BamHI BbvI BfaI BglII
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmnI