##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbrPI
BcgI BglII BlnI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MamI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 205 CGCG CG^CG
AflII 2959 CTTAAG C^TTAAG
AocI 1035 CCTCAGG CC^T[ACGT]AGG
AspI 3660 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 716 CTCGAG C^[CT]CG[GA]G
BamHI 1765 GGATCC G^GATCC
BbeI 3883 GGCGCC GGCGC^C
BclI 2995 TGATCA T^GATCA
BfrI 2959 CTTAAG C^TTAAG
BsaAI 3425 TACGTG [CT]AC^GT[GA]
BsaI 1261 GAGACC GGTCTC(1/5)
BsgI 3771 GTGCAG GTGCAG(16/14)
BsiEI 395 CGGCCG CG[GA][CT]^CG
BsoBI 716 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3832 TGTACA T^GTACA
BsrBI 4030 GAGCGG CCGCTC(-3/-3)
BsrDI 2517 GCAATG GCAATG(2/0)
BsrGI 3832 TGTACA T^GTACA
BstUI 205 CGCG CG^CG
Bsu36I 1035 CCTCAGG CC^T[ACGT]AGG
CfoI 3884 GCGC GCG^C
DraI 613 TTTAAA TTT^AAA
DraIII 3638 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 395 CGGCCG C^GGCCG
EclXI 395 CGGCCG C^GGCCG
Eco52I 395 CGGCCG C^GGCCG
Eco81I 1035 CCTCAGG CC^T[ACGT]AGG
EcoNI 627 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 4032 GCGGG CCCGC(4/6)
FbaI 2995 TGATCA T^GATCA
HaeII 3883 GGCGCC [GA]GCGC^[CT]
HhaI 3884 GCGC GCG^C
HinP1I 3884 GCGC G^CGC
HindIII 2547 AAGCTT A^AGCTT
KasI 3883 GGCGCC G^GCGCC
MfeI 3237 CAATTG C^AATTG
MunI 3237 CAATTG C^AATTG
MvnI 205 CGCG CG^CG
NarI 3883 GGCGCC GG^CGCC
PaeR7I 716 CTCGAG C^TCGAG
PflFI 3660 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3637 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1521 AGGTCCC [GA]G^G[AT]CC[CT]
SalI 268 GTCGAC G^TCGAC
ScaI 3305 AGTACT AGT^ACT
SfoI 3883 GGCGCC GGC^GCC
SphI 427 GCATGC GCATG^C
SspBI 3832 TGTACA T^GTACA
SspI 2332 AATATT AAT^ATT
Tth111I 3660 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3637 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 716 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 3063 GAATTT [GA]^AATT[CT]
3861 AAATTC
ApoI 3063 GAATTT [GA]^AATT[CT]
3861 AAATTC
BciVI 1496 GGATAC GTATCC(6/5)
2378 GGATAC
BcnI 3791 CCGGG CC^[GC]GG
3880 CCCGG
BsmBI 201 GAGACG CGTCTC(1/5)
1066 GAGACG
BsmI 3445 GAATGC GAATGC(1/-1)
3584 GCATTC
BspHI 152 TCATGA T^CATGA
2050 TCATGA
BstYI 1765 GGATCC [GA]^GATC[CT]
2790 GGATCT
DrdI 1222 GACAGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3911 GACCACGCTGTC
MflI 1765 GGATCC [GA]^GATC[CT]
2790 GGATCT
NciI 3791 CCGGG CC^[GC]GG
3880 CCCGG
PleI 1367 GACTC GAGTC(4/5)
2054 GACTC
RcaI 152 TCATGA T^CATGA
2050 TCATGA
SacI 1097 GAGCTC GAGCT^C
3708 GAGCTC
SexAI 371 ACCTGGT A^CC[AT]GGT
2638 ACCAGGT
SfiI 255 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2710 GGCCAGTGAGGCC
XhoII 1765 GGATCC [GA]^GATC[CT]
2790 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I CelII Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI