##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AspI AviII BciVI BfrI BglII
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MroI MunI NdeI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfiI SfuI
SpeI SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1056 GTAGAC GT^[AC][GT]AC
Asp700I 4409 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3398 GGATCC G^GATCC
BbvCI 1751 GCTGAGG CCTCAGC(-5/-2)
BcgI 4128 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1327 TGATCA T^GATCA
BsmI 480 GAATGC GAATGC(1/-1)
BspLU11I 871 ACATGT A^CATGT
BsrDI 593 CATTGC GCAATG(2/0)
BssHII 1772 GCGCGC G^CGCGC
BstEII 2096 GGTGACC G^GT[ACGT]ACC
BstPI 2096 GGTGACC G^GT[ACGT]ACC
DraIII 1824 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 121 GACTGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2096 GGTGACC G^GT[ACGT]ACC
FbaI 1327 TGATCA T^GATCA
MluI 1834 ACGCGT A^CGCGT
NaeI 3344 GCCGGC GCC^GGC
NgoMIV 3344 GCCGGC G^CCGGC
NheI 1452 GCTAGC G^CTAGC
NotI 4742 GCGGCCGC GC^GGCCGC
PaeR7I 3530 CTCGAG C^TCGAG
PflMI 2906 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 3102 CCTGCAGG CCTGCA^GG
SexAI 4568 ACCTGGT A^CC[AT]GGT
SgrAI 1465 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 3669 TACGTA TAC^GTA
SphI 1840 GCATGC GCATG^C
Sse8387I 3102 CCTGCAGG CCTGCA^GG
Van91I 2906 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3709 TCTAGA T^CTAGA
XhoI 3530 CTCGAG C^TCGAG
XmnI 4409 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 495 AAATTT [GA]^AATT[CT]
1947 GAATTT
Alw44I 2326 GTGCAC G^TGCAC
4171 GTGCAC
ApaLI 2326 GTGCAC G^TGCAC
4171 GTGCAC
ApoI 495 AAATTT [GA]^AATT[CT]
1947 GAATTT
AvrII 3552 CCTAGG C^CTAGG
4066 CCTAGG
BbrPI 1646 CACGTG CAC^GTG
1827 CACGTG
BlnI 3552 CCTAGG C^CTAGG
4066 CCTAGG
BlpI 1685 GCTGAGC GC^T[ACGT]AGC
3524 GCTCAGC
Bpu1102I 1685 GCTGAGC GC^T[ACGT]AGC
3524 GCTCAGC
BsaWI 409 ACCGGA [AT]^CCGG[AT]
2675 TCCGGT
BsrBI 2687 CCGCTC CCGCTC(-3/-3)
1857 GAGCGG
BsrFI 1466 GCCGGT [GA]^CCGG[CT]
3344 GCCGGC
BssSI 2797 CACGAG CACGAG(-5/-1)
2151 CTCGTG
BstXI 2249 CCAGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2536 CCATGGTTGTGG
BstYI 2084 GGATCT [GA]^GATC[CT]
3398 GGATCC
CelII 1685 GCTGAGC GC^T[ACGT]AGC
3524 GCTCAGC
Cfr10I 1466 GCCGGT [GA]^CCGG[CT]
3344 GCCGGC
EcoT22I 258 ATGCAT ATGCA^T
3375 ATGCAT
MflI 2084 GGATCT [GA]^GATC[CT]
3398 GGATCC
NsiI 258 ATGCAT ATGCA^T
3375 ATGCAT
PmaCI 1646 CACGTG CAC^GTG
1827 CACGTG
PmlI 1646 CACGTG CAC^GTG
1827 CACGTG
PshAI 2126 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2583 GACAGCGGTC
SapI 413 GAAGAGC GCTCTTC(1/4)
1547 GCTCTTC
XhoII 2084 GGATCT [GA]^GATC[CT]
3398 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
Aor51HI ApaI AspEI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI