##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII BbeI BbrPI BcgI
BclI BfrI BsaBI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KasI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
ScaI SfoI SfuI SgrAI SnaBI
SpeI SspBI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 367 GGTACC G^GTACC
AccIII 4051 TCCGGA T^CCGGA
AcsI 2625 GAATTT [GA]^AATT[CT]
AcyI 2097 GACGCC G[GA]^CG[CT]C
AgeI 799 ACCGGT A^CCGGT
Alw44I 1425 GTGCAC G^TGCAC
ApaLI 1425 GTGCAC G^TGCAC
ApoI 2625 GAATTT [GA]^AATT[CT]
Asp700I 830 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 367 GGTACC G^GTACC
AvrII 4070 CCTAGG C^CTAGG
BamHI 2512 GGATCC G^GATCC
BbsI 3020 GAAGAC GAAGAC(2/6)
BglII 466 AGATCT A^GATCT
BlnI 4070 CCTAGG C^CTAGG
BpuAI 3020 GAAGAC GAAGAC(2/6)
BsaAI 3072 TACGTG [CT]AC^GT[GA]
BsaHI 2097 GACGCC G[GA]^CG[CT]C
BseAI 4051 TCCGGA T^CCGGA
BsiEI 2201 CGACCG CG[GA][CT]^CG
BsmI 1211 GAATGC GAATGC(1/-1)
BspEI 4051 TCCGGA T^CCGGA
Hin1I 2097 GACGCC G[GA]^CG[CT]C
HindIII 609 AAGCTT A^AGCTT
KpnI 367 GGTACC GGTAC^C
MroI 4051 TCCGGA T^CCGGA
NcoI 2941 CCATGG C^CATGG
PinAI 799 ACCGGT A^CCGGT
SalI 2394 GTCGAC G^TCGAC
SfiI 2027 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2236 CCCGGG CCC^GGG
SphI 3040 GCATGC GCATG^C
Tsp509I 2626 AATT ^AATT
XmaCI 2236 CCCGGG C^CCGGG
XmaI 2236 CCCGGG C^CCGGG
XmnI 830 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 441 GTCTAC GT^[AC][GT]AC
2394 GTCGAC
BalI 3112 TGGCCA TGG^CCA
4344 TGGCCA
BbvCI 922 CCTCAGC CCTCAGC(-5/-2)
1301 GCTGAGG
BciVI 2147 GGATAC GTATCC(6/5)
654 GTATCC
BsaI 394 GAGACC GGTCTC(1/5)
3772 GGTCTC
BsaWI 799 ACCGGT [AT]^CCGG[AT]
4051 TCCGGA
BsmBI 331 GAGACG CGTCTC(1/5)
2991 GAGACG
BssHII 1380 GCGCGC G^CGCGC
1636 GCGCGC
HincII 552 GTCAAC GT[CT]^[GA]AC
2394 GTCGAC
HindII 552 GTCAAC GT[CT]^[GA]AC
2394 GTCGAC
MluNI 3112 TGGCCA TGG^CCA
4344 TGGCCA
MscI 3112 TGGCCA TGG^CCA
4344 TGGCCA
MseI 3536 TTAA T^TAA
3565 TTAA
PshAI 545 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACGAGGGTC
SexAI 2014 ACCTGGT A^CC[AT]GGT
3340 ACCTGGT
SspI 3538 AATATT AAT^ATT
3567 AATATT
Tru9I 3536 TTAA T^TAA
3565 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmlI Sse8387I StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII