##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AccIII      AclI        AflIII      AhdI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BalI        BbrPI       BcgI        
BciVI       BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BseAI       BsiWI       BspDI       BspEI       
BspLU11I    Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRI       
FseI        FspI        HindIII     MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NdeI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        ScaI        SfiI        
SfuI        SnaBI       Sse8387I    StuI        SwaI        
Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        3306  GACGTC               GACGT^C
AflII        4078  CTTAAG               C^TTAAG
AgeI         1221  ACCGGT               A^CCGGT
Alw44I       2675  GTGCAC               G^TGCAC
AocI         3260  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2188  AGCGCT               AGC^GCT
ApaLI        2675  GTGCAC               G^TGCAC
BamHI         583  GGATCC               G^GATCC
BbsI         1155  GTCTTC               GAAGAC(2/6)
BclI         4111  TGATCA               T^GATCA
BfrI         4078  CTTAAG               C^TTAAG
BpuAI        1155  GTCTTC               GAAGAC(2/6)
BsaBI         787  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         129  CGGTCG               CG[GA][CT]^CG
BssHII        124  GCGCGC               G^CGCGC
BssSI         977  CACGAG               CACGAG(-5/-1)
BstXI        2644  CCAGCAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3260  CCTGAGG              CC^T[ACGT]AGG
DraIII       2669  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          975  GACACGAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2188  AGCGCT               AGC^GCT
Eco81I       3260  CCTGAGG              CC^T[ACGT]AGG
EcoRV        1927  GATATC               GAT^ATC
FbaI         4111  TGATCA               T^GATCA
HpaI         3569  GTTAAC               GTT^AAC
KspI           60  CCGCGG               CCGC^GG
MamI          787  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI         1777  GCTAGC               G^CTAGC
PinAI        1221  ACCGGT               A^CCGGT
PpuMI        2927  AGGACCC              [GA]G^G[AT]CC[CT]
SacI         3946  GAGCTC               GAGCT^C
SacII          60  CCGCGG               CCGC^GG
SapI         4137  GCTCTTC              GCTCTTC(1/4)
SexAI        2650  ACCTGGT              A^CC[AT]GGT
SgrAI         143  CACCGGCG             C[GA]^CCGG[CT]G
SpeI         3921  ACTAGT               A^CTAGT
SphI         2786  GCATGC               GCATG^C
TaqI          899  TCGA                 T^CGA
TthHB8I       899  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AvaI          106  CCCGGG               C^[CT]CG[GA]G
              167  CCCGGG              
BglII         849  AGATCT               A^GATCT
             2577  AGATCT              
BsaWI        1221  ACCGGT               [AT]^CCGG[AT]
             1791  TCCGGT              
BsmBI        1178  CGTCTC               CGTCTC(1/5)
             1069  GAGACG              
BsmI         2589  GAATGC               GAATGC(1/-1)
             2765  GAATGC              
BsoBI         106  CCCGGG               C^[CT]CG[GA]G
              167  CCCGGG              
Bsp1407I     3332  TGTACA               T^GTACA
             4017  TGTACA              
BspHI         336  TCATGA               T^CATGA
             3770  TCATGA              
BsrDI        1732  GCAATG               GCAATG(2/0)
             2479  GCAATG              
BsrGI        3332  TGTACA               T^GTACA
             4017  TGTACA              
EaeI           83  CGGCCA               [CT]^GGCC[GA]
              419  TGGCCG              
HgaI          228  GACGC                GACGC(5/10)
             1071  GACGC               
HincII       2607  GTTGAC               GT[CT]^[GA]AC
             3569  GTTAAC              
HindII       2607  GTTGAC               GT[CT]^[GA]AC
             3569  GTTAAC              
NcoI          588  CCATGG               C^CATGG
             2426  CCATGG              
PstI         1598  CTGCAG               CTGCA^G
             1967  CTGCAG              
RcaI          336  TCATGA               T^CATGA
             3770  TCATGA              
SmaI          106  CCCGGG               CCC^GGG
              167  CCCGGG              
SmlI          994  CTCAAG               C^T[CT][GA]AG
             4078  CTTAAG              
SspBI        3332  TGTACA               T^GTACA
             4017  TGTACA              
XmaCI         106  CCCGGG               C^CCGGG
              167  CCCGGG              
XmaI          106  CCCGGG               C^CCGGG
              167  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcsI        
AcyI        AfaI        AluI        AlwI        AlwNI       
ApaI        ApoI        Asp718I     AspHI       AvaII       
BanI        BanII       BbeI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PflMI       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SspI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XbaI        XcmI        XhoII