################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AccIII AclI AflIII AhdI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BbrPI BcgI BciVI BglI BlnI BlpI Bpu1102I BsaAI BseAI BsiWI BspDI BspEI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI FseI FspI HindIII MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfuI SnaBI Sse8387I StuI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 3306 GACGTC GACGT^C AflII 4078 CTTAAG C^TTAAG AgeI 1221 ACCGGT A^CCGGT Alw44I 2675 GTGCAC G^TGCAC AocI 3260 CCTGAGG CC^T[ACGT]AGG Aor51HI 2188 AGCGCT AGC^GCT ApaLI 2675 GTGCAC G^TGCAC BamHI 583 GGATCC G^GATCC BbsI 1155 GTCTTC GAAGAC(2/6) BclI 4111 TGATCA T^GATCA BfrI 4078 CTTAAG C^TTAAG BpuAI 1155 GTCTTC GAAGAC(2/6) BsaBI 787 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 129 CGGTCG CG[GA][CT]^CG BssHII 124 GCGCGC G^CGCGC BssSI 977 CACGAG CACGAG(-5/-1) BstXI 2644 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 3260 CCTGAGG CC^T[ACGT]AGG DraIII 2669 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 975 GACACGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2188 AGCGCT AGC^GCT Eco81I 3260 CCTGAGG CC^T[ACGT]AGG EcoRV 1927 GATATC GAT^ATC FbaI 4111 TGATCA T^GATCA HpaI 3569 GTTAAC GTT^AAC KspI 60 CCGCGG CCGC^GG MamI 787 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 1777 GCTAGC G^CTAGC PinAI 1221 ACCGGT A^CCGGT PpuMI 2927 AGGACCC [GA]G^G[AT]CC[CT] SacI 3946 GAGCTC GAGCT^C SacII 60 CCGCGG CCGC^GG SapI 4137 GCTCTTC GCTCTTC(1/4) SexAI 2650 ACCTGGT A^CC[AT]GGT SgrAI 143 CACCGGCG C[GA]^CCGG[CT]G SpeI 3921 ACTAGT A^CTAGT SphI 2786 GCATGC GCATG^C TaqI 899 TCGA T^CGA TthHB8I 899 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaI 106 CCCGGG C^[CT]CG[GA]G 167 CCCGGG BglII 849 AGATCT A^GATCT 2577 AGATCT BsaWI 1221 ACCGGT [AT]^CCGG[AT] 1791 TCCGGT BsmBI 1178 CGTCTC CGTCTC(1/5) 1069 GAGACG BsmI 2589 GAATGC GAATGC(1/-1) 2765 GAATGC BsoBI 106 CCCGGG C^[CT]CG[GA]G 167 CCCGGG Bsp1407I 3332 TGTACA T^GTACA 4017 TGTACA BspHI 336 TCATGA T^CATGA 3770 TCATGA BsrDI 1732 GCAATG GCAATG(2/0) 2479 GCAATG BsrGI 3332 TGTACA T^GTACA 4017 TGTACA EaeI 83 CGGCCA [CT]^GGCC[GA] 419 TGGCCG HgaI 228 GACGC GACGC(5/10) 1071 GACGC HincII 2607 GTTGAC GT[CT]^[GA]AC 3569 GTTAAC HindII 2607 GTTGAC GT[CT]^[GA]AC 3569 GTTAAC NcoI 588 CCATGG C^CATGG 2426 CCATGG PstI 1598 CTGCAG CTGCA^G 1967 CTGCAG RcaI 336 TCATGA T^CATGA 3770 TCATGA SmaI 106 CCCGGG CCC^GGG 167 CCCGGG SmlI 994 CTCAAG C^T[CT][GA]AG 4078 CTTAAG SspBI 3332 TGTACA T^GTACA 4017 TGTACA XmaCI 106 CCCGGG C^CCGGG 167 CCCGGG XmaI 106 CCCGGG C^CCGGG 167 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp718I AspHI AvaII BanI BanII BbeI BbvCI BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoII