##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI BciVI
BsaBI BsiEI BsiWI BspDI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI Sse8387I SspI SwaI Tsp509I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 275 TCCGGA T^CCGGA
AflII 1304 CTTAAG C^TTAAG
Asp700I 2131 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 268 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3741 TGCGCA TGC^GCA
AvrII 1783 CCTAGG C^CTAGG
BalI 344 TGGCCA TGG^CCA
BcgI 312 CGACTCTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1304 CTTAAG C^TTAAG
BlnI 1783 CCTAGG C^CTAGG
BseAI 275 TCCGGA T^CCGGA
BsmI 2255 GCATTC GAATGC(1/-1)
Bsp1407I 359 TGTACA T^GTACA
BspEI 275 TCCGGA T^CCGGA
BspHI 2458 TCATGA T^CATGA
BspLU11I 2483 ACATGT A^CATGT
BsrBI 1004 CCGCTC CCGCTC(-3/-3)
BsrGI 359 TGTACA T^GTACA
BssHII 11 GCGCGC G^CGCGC
CpoI 89 CGGACCG CG^G[AT]CCG
DrdI 4237 GACTGATTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4248 CTCTTC CTCTTC(1/4)
EcoNI 3472 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3179 ATGCAT ATGCA^T
FspI 3741 TGCGCA TGC^GCA
Ksp632I 4248 CTCTTC CTCTTC(1/4)
MluNI 344 TGGCCA TGG^CCA
MroI 275 TCCGGA T^CCGGA
MscI 344 TGGCCA TGG^CCA
NaeI 907 GCCGGC GCC^GGC
NcoI 1446 CCATGG C^CATGG
NgoMIV 907 GCCGGC G^CCGGC
NsiI 3179 ATGCAT ATGCA^T
PflFI 268 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3900 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2458 TCATGA T^CATGA
RsrII 89 CGGACCG CG^G[AT]CCG
SgrAI 672 CACCGGCG C[GA]^CCGG[CT]G
SphI 2786 GCATGC GCATG^C
SspBI 359 TGTACA T^GTACA
Tth111I 268 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1208 TCTAGA T^CTAGA
XmnI 2131 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 532 GTGCAC G^TGCAC
2563 GTGCAC
ApaLI 532 GTGCAC G^TGCAC
2563 GTGCAC
BbeI 2941 GGCGCC GGCGC^C
3198 GGCGCC
BglI 3630 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4047 GCCACATGGGC
BsaWI 275 TCCGGA [AT]^CCGG[AT]
3497 ACCGGA
BsrDI 3662 CATTGC GCAATG(2/0)
3738 CATTGC
BsrFI 673 ACCGGC [GA]^CCGG[CT]
907 GCCGGC
BstAPI 2525 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3216 GCACCCTCTGC
Cfr10I 673 ACCGGC [GA]^CCGG[CT]
907 GCCGGC
EaeI 344 TGGCCA [CT]^GGCC[GA]
905 TGGCCG
HaeII 2941 GGCGCC [GA]GCGC^[CT]
3198 GGCGCC
HincII 715 GTTGAC GT[CT]^[GA]AC
1936 GTTGAC
HindII 715 GTTGAC GT[CT]^[GA]AC
1936 GTTGAC
KasI 2941 GGCGCC G^GCGCC
3198 GGCGCC
NarI 2941 GGCGCC GG^CGCC
3198 GGCGCC
PaeR7I 957 CTCGAG C^TCGAG
2798 CTCGAG
SfoI 2941 GGCGCC GGC^GCC
3198 GGCGCC
XcmI 2935 CCACCTGGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4210 CCAGGACATGCCTGG
XhoI 957 CTCGAG C^TCGAG
2798 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BamHI BanI
BanII BbrPI BbsI BbvCI BbvI
BclI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
Eco81I EcoO109I EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI