##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AvrII
BbeI BbrPI BcgI BglI BlnI
BsaAI BsaHI BseAI BsiWI BsmBI
BspDI BspEI BspMI BssHII BstAPI
BstEII BstPI Bsu36I ClaI CpoI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRV FseI Hin1I KasI KpnI
MluI MroI NarI NheI NotI
NruI PacI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Alw44I 2210 GTGCAC G^TGCAC
AlwNI 2728 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2210 GTGCAC G^TGCAC
AviII 2975 TGCGCA TGC^GCA
BamHI 30 GGATCC G^GATCC
BclI 3692 TGATCA T^GATCA
BlpI 2464 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2464 GCTGAGC GC^T[ACGT]AGC
BsaBI 1729 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 149 CGGCCG CG[GA][CT]^CG
BspLU11I 3482 ACATGT A^CATGT
BsrDI 2922 CATTGC GCAATG(2/0)
Bst1107I 4385 GTATAC GTA^TAC
BstBI 505 TTCGAA TT^CGAA
BstZ17I 4385 GTATAC GTA^TAC
CelII 2464 GCTGAGC GC^T[ACGT]AGC
EagI 149 CGGCCG C^GGCCG
EclXI 149 CGGCCG C^GGCCG
Eco52I 149 CGGCCG C^GGCCG
EcoT22I 2946 ATGCAT ATGCA^T
FbaI 3692 TGATCA T^GATCA
FspI 2975 TGCGCA TGC^GCA
HaeII 16 GGCGCT [GA]GCGC^[CT]
HpaI 3702 GTTAAC GTT^AAC
KspI 23 CCGCGG CCGC^GG
MamI 1729 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 348 CAATTG C^AATTG
MunI 348 CAATTG C^AATTG
NaeI 1643 GCCGGC GCC^GGC
NgoMIV 1643 GCCGGC G^CCGGC
NsiI 2946 ATGCAT ATGCA^T
NspV 505 TTCGAA TT^CGAA
PaeR7I 2799 CTCGAG C^TCGAG
PpuMI 356 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 3664 CAGCTG CAG^CTG
SacII 23 CCGCGG CCGC^GG
SapI 1420 GCTCTTC GCTCTTC(1/4)
ScaI 2044 AGTACT AGT^ACT
SexAI 595 ACCTGGT A^CC[AT]GGT
SfuI 505 TTCGAA TT^CGAA
XbaI 2077 TCTAGA T^CTAGA
XcmI 3656 CCAAAACACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2799 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3060 GTCTAC GT^[AC][GT]AC
4385 GTATAC
AclI 2364 AACGTT AA^CGTT
2771 AACGTT
AflII 3410 CTTAAG C^TTAAG
3457 CTTAAG
AflIII 323 ACACGT A^C[GA][CT]GT
3482 ACATGT
AspI 701 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2275 GACAGAGTC
AvaII 357 GGACC G^G[AT]CC
372 GGACC
BanI 1247 GGCACC G^G[CT][GA]CC
2792 GGTGCC
BbvCI 2805 CCTCAGC CCTCAGC(-5/-2)
241 GCTGAGG
BciVI 999 GTATCC GTATCC(6/5)
3848 GTATCC
BcnI 178 CCGGG CC^[GC]GG
1226 CCCGG
BfrI 3410 CTTAAG C^TTAAG
3457 CTTAAG
BglII 417 AGATCT A^GATCT
1883 AGATCT
BsaI 472 GAGACC GGTCTC(1/5)
2884 GAGACC
BsaWI 977 ACCGGA [AT]^CCGG[AT]
2564 ACCGGA
BsgI 4193 CTGCAC GTGCAG(16/14)
39 GTGCAG
BspHI 684 TCATGA T^CATGA
1143 TCATGA
BsrFI 1559 ACCGGC [GA]^CCGG[CT]
1643 GCCGGC
BssSI 1834 CACGAG CACGAG(-5/-1)
1975 CACGAG
BstXI 399 CCACCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4006 CCAGTGAGATGG
Cfr10I 1559 ACCGGC [GA]^CCGG[CT]
1643 GCCGGC
DraI 2173 TTTAAA TTT^AAA
2257 TTTAAA
DraIII 3247 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4075 CACTAAGTG
EcoNI 427 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3107 CCTTGGGAAGG
MspA1I 23 CCGCGG C[AC]G^C[GT]G
3664 CAGCTG
NciI 178 CCGGG CC^[GC]GG
1226 CCCGG
NcoI 236 CCATGG C^CATGG
740 CCATGG
PflFI 701 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2275 GACAGAGTC
PflMI 2854 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4074 CCACTAAGTGG
Psp1406I 2364 AACGTT AA^CGTT
2771 AACGTT
PstI 1953 CTGCAG CTGCA^G
4497 CTGCAG
RcaI 684 TCATGA T^CATGA
1143 TCATGA
StuI 1550 AGGCCT AGG^CCT
2447 AGGCCT
Tth111I 701 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2275 GACAGAGTC
Van91I 2854 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4074 CCACTAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
BalI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrBI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI