##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AviII AvrII BamHI
BbeI BbvCI BcgI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspLU11I BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HaeII Hin1I KasI KpnI
KspI MluI MvnI NarI NcoI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 198 TCCGGA T^CCGGA
AclI 1186 AACGTT AA^CGTT
AflII 3500 CTTAAG C^TTAAG
AlwI 472 GGATC GGATC(4/5)
AspI 1556 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 288 TGGCCA TGG^CCA
BanII 1 GAGCCC G[GA]GC[CT]^C
BbrPI 1430 CACGTG CAC^GTG
BciVI 2875 GGATAC GTATCC(6/5)
BclI 1669 TGATCA T^GATCA
BfrI 3500 CTTAAG C^TTAAG
BmyI 1 GAGCCC G[AGT]GC[ACT]^C
BsaAI 1430 CACGTG [CT]AC^GT[GA]
BsaWI 198 TCCGGA [AT]^CCGG[AT]
BseAI 198 TCCGGA T^CCGGA
BsmI 1620 GCATTC GAATGC(1/-1)
Bsp1286I 1 GAGCCC G[AGT]GC[ACT]^C
BspEI 198 TCCGGA T^CCGGA
BsrDI 1406 GCAATG GCAATG(2/0)
BsrFI 43 GCCGGC [GA]^CCGG[CT]
BssSI 986 CTCGTG CACGAG(-5/-1)
BstXI 3115 CCACAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 472 GGATCT [GA]^GATC[CT]
CfoI 1404 GCGC GCG^C
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
EaeI 288 TGGCCA [CT]^GGCC[GA]
EcoNI 1027 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3346 ATGCAT ATGCA^T
FauI 156 GCGGG CCCGC(4/6)
FbaI 1669 TGATCA T^GATCA
HgaI 1560 GCGTC GACGC(5/10)
HhaI 1404 GCGC GCG^C
HinP1I 1404 GCGC G^CGC
HpaI 2642 GTTAAC GTT^AAC
MfeI 1961 CAATTG C^AATTG
MflI 472 GGATCT [GA]^GATC[CT]
MluNI 288 TGGCCA TGG^CCA
MroI 198 TCCGGA T^CCGGA
MscI 288 TGGCCA TGG^CCA
MunI 1961 CAATTG C^AATTG
NaeI 43 GCCGGC GCC^GGC
NdeI 3850 CATATG CA^TATG
NgoMIV 43 GCCGGC G^CCGGC
NsiI 3346 ATGCAT ATGCA^T
PflFI 1556 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1430 CACGTG CAC^GTG
PmlI 1430 CACGTG CAC^GTG
Psp1406I 1186 AACGTT AA^CGTT
PvuII 2545 CAGCTG CAG^CTG
SapI 739 GAAGAGC GCTCTTC(1/4)
SexAI 1017 ACCTGGT A^CC[AT]GGT
SmaI 4 CCCGGG CCC^GGG
Tth111I 1556 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1772 TCTAGA T^CTAGA
XhoII 472 GGATCT [GA]^GATC[CT]
XmaCI 4 CCCGGG C^CCGGG
XmaI 4 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AseI 2471 ATTAAT AT^TAAT
3600 ATTAAT
AsnI 2471 ATTAAT AT^TAAT
3600 ATTAAT
Asp700I 1267 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4139 GAAAGTATTC
AvaI 4 CCCGGG C^[CT]CG[GA]G
2988 CCCGAG
BsaBI 1105 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4067 GATTACCATC
BsgI 1892 CTGCAC GTGCAG(16/14)
108 GTGCAG
BsmAI 29 GAGAC GTCTC(1/5)
937 GTCTC
BsoBI 4 CCCGGG C^[CT]CG[GA]G
2988 CCCGAG
BspHI 699 TCATGA T^CATGA
1172 TCATGA
DraIII 655 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
983 CACCTCGTG
EcoT14I 1211 CCAAGG C^C[AT][AT]GG
1486 CCAAGG
HaeIII 289 GGCC GG^CC
1543 GGCC
HindIII 1228 AAGCTT A^AGCTT
3129 AAGCTT
MamI 1105 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4067 GATTACCATC
MspA1I 142 CCGCTG C[AC]G^C[GT]G
2545 CAGCTG
NspI 1845 ACATGC [GA]CATG^[CT]
3348 GCATGT
PshBI 2471 ATTAAT AT^TAAT
3600 ATTAAT
PstI 3882 CTGCAG CTGCA^G
4204 CTGCAG
RcaI 699 TCATGA T^CATGA
1172 TCATGA
ScaI 3551 AGTACT AGT^ACT
3886 AGTACT
SfcI 3882 CTGCAG C^T[GA][CT]AG
4204 CTGCAG
SmlI 375 CTTGAG C^T[CT][GA]AG
3500 CTTAAG
StyI 1211 CCAAGG C^C[AT][AT]GG
1486 CCAAGG
XmnI 1267 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4139 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BanI BbsI BbvI
BcnI BfaI BpuAI BsaJI BseRI
BsiYI BslI BsmFI Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII Fnu4HI FokI HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I