##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII BbeI BbrPI BciVI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII Eam1105I
Eco47III EcoNI EcoO65I EcoT22I FseI
FspI Hin1I KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI SacII
SexAI SfiI SfoI SgrAI SmaI
SnaBI SspBI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 140 TCCGGA T^CCGGA
Asp700I 3467 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3556 CCTAGG C^CTAGG
BanI 1054 GGCACC G^G[CT][GA]CC
BbvCI 884 GCTGAGG CCTCAGC(-5/-2)
BlnI 3556 CCTAGG C^CTAGG
BsaWI 140 TCCGGA [AT]^CCGG[AT]
BseAI 140 TCCGGA T^CCGGA
BsiEI 3 CGGCCG CG[GA][CT]^CG
BspDI 687 ATCGAT AT^CGAT
BspEI 140 TCCGGA T^CCGGA
BspHI 2001 TCATGA T^CATGA
BsrBI 62 GAGCGG CCGCTC(-3/-3)
BsrFI 102 ACCGGC [GA]^CCGG[CT]
BstAPI 363 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 832 TTCGAA TT^CGAA
Cfr10I 102 ACCGGC [GA]^CCGG[CT]
ClaI 687 ATCGAT AT^CGAT
CpoI 142 CGGACCG CG^G[AT]CCG
DraI 3447 TTTAAA TTT^AAA
DraIII 3283 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3901 GACTCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 425 CCGTGG C^C[GA][CT]GG
EagI 3 CGGCCG C^GGCCG
EclXI 3 CGGCCG C^GGCCG
Eco52I 3 CGGCCG C^GGCCG
EcoRV 1787 GATATC GAT^ATC
HaeII 1272 AGCGCC [GA]GCGC^[CT]
HgaI 280 GCGTC GACGC(5/10)
HindIII 1320 AAGCTT A^AGCTT
HpaI 1518 GTTAAC GTT^AAC
MroI 140 TCCGGA T^CCGGA
NspV 832 TTCGAA TT^CGAA
RcaI 2001 TCATGA T^CATGA
RsrII 142 CGGACCG CG^G[AT]CCG
SacI 2134 GAGCTC GAGCT^C
SalI 258 GTCGAC G^TCGAC
SapI 1634 GCTCTTC GCTCTTC(1/4)
SbfI 3384 CCTGCAGG CCTGCA^GG
ScaI 4011 AGTACT AGT^ACT
SfuI 832 TTCGAA TT^CGAA
SpeI 39 ACTAGT A^CTAGT
Sse8387I 3384 CCTGCAGG CCTGCA^GG
TfiI 1670 GAATC G^A[AT]TC
XmnI 3467 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 258 GTCGAC GT^[AC][GT]AC
2116 GTAGAC
AflIII 1522 ACATGT A^C[GA][CT]GT
3700 ACATGT
ApaI 646 GGGCCC GGGCC^C
1227 GGGCCC
AseI 3585 ATTAAT AT^TAAT
3759 ATTAAT
AsnI 3585 ATTAAT AT^TAAT
3759 ATTAAT
AspHI 2134 GAGCTC G[AT]GC[AT]^C
3420 GTGCTC
BalI 866 TGGCCA TGG^CCA
1366 TGGCCA
BamHI 251 GGATCC G^GATCC
1841 GGATCC
BcgI 147 CGAGACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1157 CGACAGAATTGC
BglII 2569 AGATCT A^GATCT
3077 AGATCT
BsiHKAI 2134 GAGCTC G[AT]GC[AT]^C
3420 GTGCTC
BspLU11I 1522 ACATGT A^CATGT
3700 ACATGT
BspMI 1469 ACCTGC ACCTGC(4/8)
3383 ACCTGC
FauI 270 CCCGC CCCGC(4/6)
2292 CCCGC
HincII 258 GTCGAC GT[CT]^[GA]AC
1518 GTTAAC
HindII 258 GTCGAC GT[CT]^[GA]AC
1518 GTTAAC
MluNI 866 TGGCCA TGG^CCA
1366 TGGCCA
MscI 866 TGGCCA TGG^CCA
1366 TGGCCA
PshBI 3585 ATTAAT AT^TAAT
3759 ATTAAT
PstI 3058 CTGCAG CTGCA^G
3385 CTGCAG
SmlI 741 CTTGAG C^T[CT][GA]AG
2736 CTTGAG
SspI 3752 AATATT AAT^ATT
3845 AATATT
XbaI 449 TCTAGA T^CTAGA
1148 TCTAGA
XcmI 1480 CCATTCTTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2188 CCAAAGCAAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AvaII
BanII BbsI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII