##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        Alw44I      
ApaLI       AscI        AviII       BbvCI       BclI        
BfrI        BsaBI       BseAI       BspDI       BspEI       
BspHI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     ClaI        DrdI        
EcoO65I     EcoRI       EcoRV       FbaI        FspI        
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MroI        MunI        NheI        NotI        
NspV        PacI        PinAI       PmeI        Psp1406I    
RcaI        SacII       SfiI        SfuI        SgrAI       
SnaBI       SphI        SwaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        813  GGTACC               G^GTACC
AhdI         4210  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        3135  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          987  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       885  AGCGCT               AGC^GCT
AseI         4789  ATTAAT               AT^TAAT
AsnI         4789  ATTAAT               AT^TAAT
Asp718I       813  GGTACC               G^GTACC
AspEI        4210  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        4934  CCTAGG               C^CTAGG
BamHI        3361  GGATCC               G^GATCC
BcgI         4476  CGAGTTTTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        4728  GGATAC               GTATCC(6/5)
BglII        4644  AGATCT               A^GATCT
BlnI         4934  CCTAGG               C^CTAGG
BsaI         2831  GAGACC               GGTCTC(1/5)
BsaWI        1390  ACCGGA               [AT]^CCGG[AT]
BsiWI        3263  CGTACG               C^GTACG
BspLU11I     5112  ACATGT               A^CATGT
BssHII       2364  GCGCGC               G^CGCGC
BstXI         648  CCAGCTTCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        987  CCTCAGG              CC^T[ACGT]AGG
CpoI          124  CGGACCG              CG^G[AT]CCG
DraIII       4748  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2281  CGGCCG               C^GGCCG
Eam1105I     4210  GACTTACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2281  CGGCCG               C^GGCCG
Eco47III      885  AGCGCT               AGC^GCT
Eco52I       2281  CGGCCG               C^GGCCG
Eco81I        987  CCTCAGG              CC^T[ACGT]AGG
EcoT22I       714  ATGCAT               ATGCA^T
FseI          855  GGCCGGCC             GGCCGG^CC
KpnI          813  GGTACC               GGTAC^C
NdeI         1083  CATATG               CA^TATG
NruI           14  TCGCGA               TCG^CGA
NsiI          714  ATGCAT               ATGCA^T
PaeR7I        623  CTCGAG               C^TCGAG
PshAI         523  GACAGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4789  ATTAAT               AT^TAAT
PvuI         1259  CGATCG               CGAT^CG
RsrII         124  CGGACCG              CG^G[AT]CCG
SbfI         2449  CCTGCAGG             CCTGCA^GG
ScaI          744  AGTACT               AGT^ACT
SexAI         930  ACCAGGT              A^CC[AT]GGT
SpeI            2  ACTAGT               A^CTAGT
Sse8387I     2449  CCTGCAGG             CCTGCA^GG
SspI         5235  AATATT               AAT^ATT
XbaI            8  TCTAGA               T^CTAGA
XhoI          623  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          935  GTCGAC               GT^[AC][GT]AC
             2600  GTCGAC              
AflIII       2076  ACGTGT               A^C[GA][CT]GT
             5112  ACATGT              
AspI          929  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4925  GACGCAGTC           
BbeI          163  GGCGCC               GGCGC^C
              800  GGCGCC              
BbrPI         818  CACGTG               CAC^GTG
             2479  CACGTG              
BglI         1579  GCCCCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4105  GCCCTGCTGGC         
BlpI         4045  GCTCAGC              GC^T[ACGT]AGC
             4078  GCTAAGC             
Bpu1102I     4045  GCTCAGC              GC^T[ACGT]AGC
             4078  GCTAAGC             
BsmBI        1458  CGTCTC               CGTCTC(1/5)
             3228  CGTCTC              
Bsp1407I     4814  TGTACA               T^GTACA
             5115  TGTACA              
BsrBI        3374  CCGCTC               CCGCTC(-3/-3)
               18  GAGCGG              
BsrDI         717  CATTGC               GCAATG(2/0)
             2286  GCAATG              
BsrGI        4814  TGTACA               T^GTACA
             5115  TGTACA              
CelII        4045  GCTCAGC              GC^T[ACGT]AGC
             4078  GCTAAGC             
HincII        935  GTCGAC               GT[CT]^[GA]AC
             2600  GTCGAC              
HindII        935  GTCGAC               GT[CT]^[GA]AC
             2600  GTCGAC              
KasI          163  GGCGCC               G^GCGCC
              800  GGCGCC              
NarI          163  GGCGCC               GG^CGCC
              800  GGCGCC              
NspI         1782  ACATGC               [GA]CATG^[CT]
             5112  ACATGT              
PflFI         929  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4925  GACGCAGTC           
PmaCI         818  CACGTG               CAC^GTG
             2479  CACGTG              
PmlI          818  CACGTG               CAC^GTG
             2479  CACGTG              
SalI          935  GTCGAC               G^TCGAC
             2600  GTCGAC              
SapI         2621  GAAGAGC              GCTCTTC(1/4)
              301  GCTCTTC             
SfoI          163  GGCGCC               GGC^GCC
              800  GGCGCC              
SspBI        4814  TGTACA               T^GTACA
             5115  TGTACA              
TfiI         3845  GATTC                G^A[AT]TC
             4672  GAATC               
Tth111I       929  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4925  GACGCAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AatII       AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        XmnI