##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AvrII       BamHI       BclI        BglII       
BlnI        BsaBI       BsiWI       BsmBI       BspDI       
BspHI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        Eam1105I    EcoRI       
EcoRV       FbaI        HincII      HindII      HindIII     
HpaI        MamI        MfeI        MluI        MunI        
NheI        NruI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SfuI        SgrAI       
SnaBI       SpeI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        559  GGTACC               G^GTACC
AccIII       4406  TCCGGA               T^CCGGA
AcsI         3437  GAATTT               [GA]^AATT[CT]
AflII        2157  CTTAAG               C^TTAAG
Aor51HI      2923  AGCGCT               AGC^GCT
ApoI         3437  GAATTT               [GA]^AATT[CT]
Asp718I       559  GGTACC               G^GTACC
AspI         1116  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1519  TGGCCA               TGG^CCA
BbrPI        4596  CACGTG               CAC^GTG
BciVI        3952  GGATAC               GTATCC(6/5)
BfrI         2157  CTTAAG               C^TTAAG
BsaAI        4596  CACGTG               [CT]AC^GT[GA]
BseAI        4406  TCCGGA               T^CCGGA
BsmI         2959  GCATTC               GAATGC(1/-1)
BspEI        4406  TCCGGA               T^CCGGA
BspLU11I     4264  ACATGT               A^CATGT
BsrDI        2492  CATTGC               GCAATG(2/0)
BstEII       1146  GGTGACC              G^GT[ACGT]ACC
BstPI        1146  GGTGACC              G^GT[ACGT]ACC
DraI         4021  TTTAAA               TTT^AAA
Eco47III     2923  AGCGCT               AGC^GCT
EcoO65I      1146  GGTGACC              G^GT[ACGT]ACC
EcoT22I      3685  ATGCAT               ATGCA^T
FseI         1545  GGCCGGCC             GGCCGG^CC
KpnI          559  GGTACC               GGTAC^C
KspI           62  CCGCGG               CCGC^GG
MluNI        1519  TGGCCA               TGG^CCA
MroI         4406  TCCGGA               T^CCGGA
MscI         1519  TGGCCA               TGG^CCA
NdeI         3767  CATATG               CA^TATG
NotI         4814  GCGGCCGC             GC^GGCCGC
NsiI         3685  ATGCAT               ATGCA^T
PflFI        1116  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         293  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        4596  CACGTG               CAC^GTG
PmlI         4596  CACGTG               CAC^GTG
PshAI         761  GACGTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII          62  CCGCGG               CCGC^GG
SbfI         2207  CCTGCAGG             CCTGCA^GG
ScaI          328  AGTACT               AGT^ACT
SphI         4422  GCATGC               GCATG^C
Sse8387I     2207  CCTGCAGG             CCTGCA^GG
SspI         4624  AATATT               AAT^ATT
Tth111I      1116  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        293  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AccI          312  GTCTAC               GT^[AC][GT]AC
              681  GTCTAC              
AocI         1291  CCTCAGG              CC^T[ACGT]AGG
             2541  CCTGAGG             
AviII         370  TGCGCA               TGC^GCA
             2857  TGCGCA              
BcgI         2486  CGACCCCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              847  GCATGGACTTCG        
Bsu36I       1291  CCTCAGG              CC^T[ACGT]AGG
             2541  CCTGAGG             
Eco81I       1291  CCTCAGG              CC^T[ACGT]AGG
             2541  CCTGAGG             
FspI          370  TGCGCA               TGC^GCA
             2857  TGCGCA              
SfiI         1296  GGCCGGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2109  GGCCCCTTTGGCC       
SmlI         1567  CTCAAG               C^T[CT][GA]AG
             2157  CTTAAG              
StuI         4328  AGGCCT               AGG^CCT
             4396  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmaI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI