################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AvrII BamHI BclI BglII BlnI BsaBI BsiWI BsmBI BspDI BspHI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I EcoRI EcoRV FbaI HincII HindII HindIII HpaI MamI MfeI MluI MunI NheI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SfuI SgrAI SnaBI SpeI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 559 GGTACC G^GTACC AccIII 4406 TCCGGA T^CCGGA AcsI 3437 GAATTT [GA]^AATT[CT] AflII 2157 CTTAAG C^TTAAG Aor51HI 2923 AGCGCT AGC^GCT ApoI 3437 GAATTT [GA]^AATT[CT] Asp718I 559 GGTACC G^GTACC AspI 1116 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1519 TGGCCA TGG^CCA BbrPI 4596 CACGTG CAC^GTG BciVI 3952 GGATAC GTATCC(6/5) BfrI 2157 CTTAAG C^TTAAG BsaAI 4596 CACGTG [CT]AC^GT[GA] BseAI 4406 TCCGGA T^CCGGA BsmI 2959 GCATTC GAATGC(1/-1) BspEI 4406 TCCGGA T^CCGGA BspLU11I 4264 ACATGT A^CATGT BsrDI 2492 CATTGC GCAATG(2/0) BstEII 1146 GGTGACC G^GT[ACGT]ACC BstPI 1146 GGTGACC G^GT[ACGT]ACC DraI 4021 TTTAAA TTT^AAA Eco47III 2923 AGCGCT AGC^GCT EcoO65I 1146 GGTGACC G^GT[ACGT]ACC EcoT22I 3685 ATGCAT ATGCA^T FseI 1545 GGCCGGCC GGCCGG^CC KpnI 559 GGTACC GGTAC^C KspI 62 CCGCGG CCGC^GG MluNI 1519 TGGCCA TGG^CCA MroI 4406 TCCGGA T^CCGGA MscI 1519 TGGCCA TGG^CCA NdeI 3767 CATATG CA^TATG NotI 4814 GCGGCCGC GC^GGCCGC NsiI 3685 ATGCAT ATGCA^T PflFI 1116 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 293 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4596 CACGTG CAC^GTG PmlI 4596 CACGTG CAC^GTG PshAI 761 GACGTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 62 CCGCGG CCGC^GG SbfI 2207 CCTGCAGG CCTGCA^GG ScaI 328 AGTACT AGT^ACT SphI 4422 GCATGC GCATG^C Sse8387I 2207 CCTGCAGG CCTGCA^GG SspI 4624 AATATT AAT^ATT Tth111I 1116 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 293 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AccI 312 GTCTAC GT^[AC][GT]AC 681 GTCTAC AocI 1291 CCTCAGG CC^T[ACGT]AGG 2541 CCTGAGG AviII 370 TGCGCA TGC^GCA 2857 TGCGCA BcgI 2486 CGACCCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 847 GCATGGACTTCG Bsu36I 1291 CCTCAGG CC^T[ACGT]AGG 2541 CCTGAGG Eco81I 1291 CCTCAGG CC^T[ACGT]AGG 2541 CCTGAGG FspI 370 TGCGCA TGC^GCA 2857 TGCGCA SfiI 1296 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2109 GGCCCCTTTGGCC SmlI 1567 CTCAAG C^T[CT][GA]AG 2157 CTTAAG StuI 4328 AGGCCT AGG^CCT 4396 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI