##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BfrI BglI BlnI BsaWI BseAI
BsiEI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 3317 AACGTT AA^CGTT
AcyI 705 GGCGCC G[GA]^CG[CT]C
AocI 1768 CCTTAGG CC^T[ACGT]AGG
ApaI 1635 GGGCCC GGGCC^C
BalI 2284 TGGCCA TGG^CCA
BbeI 705 GGCGCC GGCGC^C
BbvCI 1833 CCTCAGC CCTCAGC(-5/-2)
BsaBI 1256 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 705 GGCGCC G[GA]^CG[CT]C
BsiWI 1328 CGTACG C^GTACG
BspLU11I 2200 ACATGT A^CATGT
BspMI 1541 GCAGGT ACCTGC(4/8)
BstXI 1294 CCACACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1768 CCTTAGG CC^T[ACGT]AGG
DraIII 1138 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 536 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1768 CCTTAGG CC^T[ACGT]AGG
HaeII 705 GGCGCC [GA]GCGC^[CT]
Hin1I 705 GGCGCC G[GA]^CG[CT]C
KasI 705 GGCGCC G^GCGCC
MamI 1256 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2284 TGGCCA TGG^CCA
MscI 2284 TGGCCA TGG^CCA
MspA1I 256 CAGCTG C[AC]G^C[GT]G
NarI 705 GGCGCC GG^CGCC
NdeI 2518 CATATG CA^TATG
NspI 2200 ACATGT [GA]CATG^[CT]
PacI 801 TTAATTAA TTAAT^TAA
Psp1406I 3317 AACGTT AA^CGTT
PstI 744 CTGCAG CTGCA^G
PvuII 256 CAGCTG CAG^CTG
ScaI 1222 AGTACT AGT^ACT
SexAI 1694 ACCAGGT A^CC[AT]GGT
SfoI 705 GGCGCC GGC^GCC
SmaI 723 CCCGGG CCC^GGG
SnaBI 3462 TACGTA TAC^GTA
XbaI 1940 TCTAGA T^CTAGA
XmaCI 723 CCCGGG C^CCGGG
XmaI 723 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2200 ACATGT A^C[GA][CT]GT
3388 ACGTGT
AlwI 1659 GGATC GGATC(4/5)
2276 GGATC
AvaI 723 CCCGGG C^[CT]CG[GA]G
1878 CCCGAG
BanI 705 GGCGCC G^G[CT][GA]CC
2445 GGTGCC
BciVI 3834 GGATAC GTATCC(6/5)
502 GTATCC
BglII 1943 AGATCT A^GATCT
2186 AGATCT
BsaAI 101 CACGTA [CT]AC^GT[GA]
3462 TACGTA
BsoBI 723 CCCGGG C^[CT]CG[GA]G
1878 CCCGAG
BsrDI 154 CATTGC GCAATG(2/0)
4044 CATTGC
CfoI 132 GCGC GCG^C
706 GCGC
EaeI 685 TGGCCG [CT]^GGCC[GA]
2284 TGGCCA
EcoRV 2531 GATATC GAT^ATC
3690 GATATC
EcoT22I 2515 ATGCAT ATGCA^T
3516 ATGCAT
FauI 787 CCCGC CCCGC(4/6)
1154 CCCGC
HhaI 132 GCGC GCG^C
706 GCGC
HinP1I 132 GCGC G^CGC
706 GCGC
NsiI 2515 ATGCAT ATGCA^T
3516 ATGCAT
PflMI 1785 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3343 CCAAACAGTGG
PpuMI 783 AGGACCC [GA]G^G[AT]CC[CT]
1282 AGGACCC
SspI 1314 AATATT AAT^ATT
3667 AATATT
Tsp45I 2239 GTGAC ^GT[GC]AC
3309 GTGAC
Van91I 1785 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3343 CCAAACAGTGG
XcmI 160 CCATGCCCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2194 CCAAGGACATGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PshBI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI