##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI BamHI
BciVI BfrI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I ClaI
Eco47III EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MroI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 4278 AAATTT [GA]^AATT[CT]
AflIII 1721 ACGCGT A^C[GA][CT]GT
AhdI 767 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4278 AAATTT [GA]^AATT[CT]
AspEI 767 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2079 TGCGCA TGC^GCA
BbrPI 3348 CACGTG CAC^GTG
BbvCI 784 CCTCAGC CCTCAGC(-5/-2)
BcgI 2352 GCATGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1677 TGATCA T^GATCA
BglII 4218 AGATCT A^GATCT
BsaAI 3348 CACGTG [CT]AC^GT[GA]
BsgI 3501 CTGCAC GTGCAG(16/14)
BsmBI 1915 GAGACG CGTCTC(1/5)
BsrDI 2552 CATTGC GCAATG(2/0)
BstAPI 3428 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1477 GGTGACC G^GT[ACGT]ACC
BstPI 1477 GGTGACC G^GT[ACGT]ACC
CpoI 169 CGGTCCG CG^G[AT]CCG
DraI 3781 TTTAAA TTT^AAA
DrdI 3289 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 767 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1729 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1477 GGTGACC G^GT[ACGT]ACC
FbaI 1677 TGATCA T^GATCA
FspI 2079 TGCGCA TGC^GCA
MluI 1721 ACGCGT A^CGCGT
NheI 3233 GCTAGC G^CTAGC
PaeR7I 1418 CTCGAG C^TCGAG
PmaCI 3348 CACGTG CAC^GTG
PmlI 3348 CACGTG CAC^GTG
RsrII 169 CGGTCCG CG^G[AT]CCG
SspI 4262 AATATT AAT^ATT
StuI 4181 AGGCCT AGG^CCT
XhoI 1418 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 781 GATCC GGATC(4/5)
325 GGATC
AocI 1209 CCTCAGG CC^T[ACGT]AGG
4177 CCTGAGG
AvrII 2242 CCTAGG C^CTAGG
3787 CCTAGG
BbsI 1658 GAAGAC GAAGAC(2/6)
2918 GTCTTC
BlnI 2242 CCTAGG C^CTAGG
3787 CCTAGG
BpuAI 1658 GAAGAC GAAGAC(2/6)
2918 GTCTTC
Bsp1407I 634 TGTACA T^GTACA
2548 TGTACA
BsrGI 634 TGTACA T^GTACA
2548 TGTACA
BssSI 2051 CACGAG CACGAG(-5/-1)
3083 CTCGTG
BstYI 325 GGATCT [GA]^GATC[CT]
4218 AGATCT
Bsu36I 1209 CCTCAGG CC^T[ACGT]AGG
4177 CCTGAGG
DraIII 3429 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3723 CACACTGTG
Eco81I 1209 CCTCAGG CC^T[ACGT]AGG
4177 CCTGAGG
MflI 325 GGATCT [GA]^GATC[CT]
4218 AGATCT
SapI 1022 GAAGAGC GCTCTTC(1/4)
1804 GCTCTTC
SfaNI 2235 GCATC GCATC(5/9)
3644 GCATC
SfiI 2999 GGCCCTAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3595 GGCCCCAAGGGCC
SphI 2717 GCATGC GCATG^C
3437 GCATGC
SspBI 634 TGTACA T^GTACA
2548 TGTACA
XhoII 325 GGATCT [GA]^GATC[CT]
4218 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI