################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AccI AccIII AclI AflIII AgeI AhdI AscI AseI AsnI AspEI AspI AviII BbeI BbrPI BciVI BclI BglII BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AatII 671 GACGTC GACGT^C AcsI 953 GAATTT [GA]^AATT[CT] AflII 3702 CTTAAG C^TTAAG AocI 2790 CCTGAGG CC^T[ACGT]AGG Aor51HI 1686 AGCGCT AGC^GCT ApoI 953 GAATTT [GA]^AATT[CT] Asp700I 706 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 2764 CCTAGG C^CTAGG BamHI 2154 GGATCC G^GATCC BcgI 2389 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3702 CTTAAG C^TTAAG BlnI 2764 CCTAGG C^CTAGG BsmBI 550 CGTCTC CGTCTC(1/5) BstAPI 1675 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2790 CCTGAGG CC^T[ACGT]AGG DraI 179 TTTAAA TTT^AAA DrdI 1270 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1686 AGCGCT AGC^GCT Eco81I 2790 CCTGAGG CC^T[ACGT]AGG FseI 1330 GGCCGGCC GGCCGG^CC KspI 1596 CCGCGG CCGC^GG NcoI 2843 CCATGG C^CATGG NspI 1957 GCATGT [GA]CATG^[CT] PaeR7I 2397 CTCGAG C^TCGAG SacII 1596 CCGCGG CCGC^GG XhoI 2397 CTCGAG C^TCGAG XmnI 706 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 639 GGTACC G^GTACC 1821 GGTACC Alw44I 1658 GTGCAC G^TGCAC 1862 GTGCAC ApaLI 1658 GTGCAC G^TGCAC 1862 GTGCAC Asp718I 639 GGTACC G^GTACC 1821 GGTACC BbvCI 1795 CCTCAGC CCTCAGC(-5/-2) 1306 GCTGAGG BglI 592 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1800 GCCTCTTCGGC BsaAI 494 TACGTG [CT]AC^GT[GA] 2131 CACGTA BsgI 3164 CTGCAC GTGCAG(16/14) 3688 CTGCAC BssSI 302 CACGAG CACGAG(-5/-1) 1049 CACGAG DraIII 1664 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG 3453 CACTGGGTG KpnI 639 GGTACC GGTAC^C 1821 GGTACC MslI 1555 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2352 CATGCAGATG NaeI 597 GCCGGC GCC^GGC 1331 GCCGGC NgoMIV 597 GCCGGC G^CCGGC 1331 GCCGGC PflMI 2137 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3037 CCAGGGTATGG SapI 462 GCTCTTC GCTCTTC(1/4) 601 GCTCTTC SexAI 635 ACCTGGT A^CC[AT]GGT 2136 ACCAGGT SmaI 909 CCCGGG CCC^GGG 2822 CCCGGG SmlI 2397 CTCGAG C^T[CT][GA]AG 3702 CTTAAG StuI 1968 AGGCCT AGG^CCT 3561 AGGCCT TaqI 2398 TCGA T^CGA 2801 TCGA Tsp509I 954 AATT ^AATT 3727 AATT TthHB8I 2398 TCGA T^CGA 2801 TCGA Van91I 2137 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3037 CCAGGGTATGG XmaCI 909 CCCGGG C^CCGGG 2822 CCCGGG XmaI 909 CCCGGG C^CCGGG 2822 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I TspRI XcmI XhoII