##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AccI AccIII AclI AflIII AgeI
AhdI AscI AseI AsnI AspEI
AspI AviII BbeI BbrPI BciVI
BclI BglII BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KasI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 671 GACGTC GACGT^C
AcsI 953 GAATTT [GA]^AATT[CT]
AflII 3702 CTTAAG C^TTAAG
AocI 2790 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1686 AGCGCT AGC^GCT
ApoI 953 GAATTT [GA]^AATT[CT]
Asp700I 706 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2764 CCTAGG C^CTAGG
BamHI 2154 GGATCC G^GATCC
BcgI 2389 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3702 CTTAAG C^TTAAG
BlnI 2764 CCTAGG C^CTAGG
BsmBI 550 CGTCTC CGTCTC(1/5)
BstAPI 1675 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2790 CCTGAGG CC^T[ACGT]AGG
DraI 179 TTTAAA TTT^AAA
DrdI 1270 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1686 AGCGCT AGC^GCT
Eco81I 2790 CCTGAGG CC^T[ACGT]AGG
FseI 1330 GGCCGGCC GGCCGG^CC
KspI 1596 CCGCGG CCGC^GG
NcoI 2843 CCATGG C^CATGG
NspI 1957 GCATGT [GA]CATG^[CT]
PaeR7I 2397 CTCGAG C^TCGAG
SacII 1596 CCGCGG CCGC^GG
XhoI 2397 CTCGAG C^TCGAG
XmnI 706 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 639 GGTACC G^GTACC
1821 GGTACC
Alw44I 1658 GTGCAC G^TGCAC
1862 GTGCAC
ApaLI 1658 GTGCAC G^TGCAC
1862 GTGCAC
Asp718I 639 GGTACC G^GTACC
1821 GGTACC
BbvCI 1795 CCTCAGC CCTCAGC(-5/-2)
1306 GCTGAGG
BglI 592 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1800 GCCTCTTCGGC
BsaAI 494 TACGTG [CT]AC^GT[GA]
2131 CACGTA
BsgI 3164 CTGCAC GTGCAG(16/14)
3688 CTGCAC
BssSI 302 CACGAG CACGAG(-5/-1)
1049 CACGAG
DraIII 1664 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
3453 CACTGGGTG
KpnI 639 GGTACC GGTAC^C
1821 GGTACC
MslI 1555 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2352 CATGCAGATG
NaeI 597 GCCGGC GCC^GGC
1331 GCCGGC
NgoMIV 597 GCCGGC G^CCGGC
1331 GCCGGC
PflMI 2137 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3037 CCAGGGTATGG
SapI 462 GCTCTTC GCTCTTC(1/4)
601 GCTCTTC
SexAI 635 ACCTGGT A^CC[AT]GGT
2136 ACCAGGT
SmaI 909 CCCGGG CCC^GGG
2822 CCCGGG
SmlI 2397 CTCGAG C^T[CT][GA]AG
3702 CTTAAG
StuI 1968 AGGCCT AGG^CCT
3561 AGGCCT
TaqI 2398 TCGA T^CGA
2801 TCGA
Tsp509I 954 AATT ^AATT
3727 AATT
TthHB8I 2398 TCGA T^CGA
2801 TCGA
Van91I 2137 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3037 CCAGGGTATGG
XmaCI 909 CCCGGG C^CCGGG
2822 CCCGGG
XmaI 909 CCCGGG C^CCGGG
2822 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I TspRI
XcmI XhoII