##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AccI        AccIII      AclI        AflIII      AgeI        
AhdI        AscI        AseI        AsnI        AspEI       
AspI        AviII       BbeI        BbrPI       BciVI       
BclI        BglII       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FspI        HincII      HindII      
HindIII     HpaI        KasI        MamI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AatII         671  GACGTC               GACGT^C
AcsI          953  GAATTT               [GA]^AATT[CT]
AflII        3702  CTTAAG               C^TTAAG
AocI         2790  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1686  AGCGCT               AGC^GCT
ApoI          953  GAATTT               [GA]^AATT[CT]
Asp700I       706  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        2764  CCTAGG               C^CTAGG
BamHI        2154  GGATCC               G^GATCC
BcgI         2389  GCAGGTGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3702  CTTAAG               C^TTAAG
BlnI         2764  CCTAGG               C^CTAGG
BsmBI         550  CGTCTC               CGTCTC(1/5)
BstAPI       1675  GCAGGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2790  CCTGAGG              CC^T[ACGT]AGG
DraI          179  TTTAAA               TTT^AAA
DrdI         1270  GACCCAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1686  AGCGCT               AGC^GCT
Eco81I       2790  CCTGAGG              CC^T[ACGT]AGG
FseI         1330  GGCCGGCC             GGCCGG^CC
KspI         1596  CCGCGG               CCGC^GG
NcoI         2843  CCATGG               C^CATGG
NspI         1957  GCATGT               [GA]CATG^[CT]
PaeR7I       2397  CTCGAG               C^TCGAG
SacII        1596  CCGCGG               CCGC^GG
XhoI         2397  CTCGAG               C^TCGAG
XmnI          706  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I        639  GGTACC               G^GTACC
             1821  GGTACC              
Alw44I       1658  GTGCAC               G^TGCAC
             1862  GTGCAC              
ApaLI        1658  GTGCAC               G^TGCAC
             1862  GTGCAC              
Asp718I       639  GGTACC               G^GTACC
             1821  GGTACC              
BbvCI        1795  CCTCAGC              CCTCAGC(-5/-2)
             1306  GCTGAGG             
BglI          592  GCCAGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1800  GCCTCTTCGGC         
BsaAI         494  TACGTG               [CT]AC^GT[GA]
             2131  CACGTA              
BsgI         3164  CTGCAC               GTGCAG(16/14)
             3688  CTGCAC              
BssSI         302  CACGAG               CACGAG(-5/-1)
             1049  CACGAG              
DraIII       1664  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3453  CACTGGGTG           
KpnI          639  GGTACC               GGTAC^C
             1821  GGTACC              
MslI         1555  CACCCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2352  CATGCAGATG          
NaeI          597  GCCGGC               GCC^GGC
             1331  GCCGGC              
NgoMIV        597  GCCGGC               G^CCGGC
             1331  GCCGGC              
PflMI        2137  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3037  CCAGGGTATGG         
SapI          462  GCTCTTC              GCTCTTC(1/4)
              601  GCTCTTC             
SexAI         635  ACCTGGT              A^CC[AT]GGT
             2136  ACCAGGT             
SmaI          909  CCCGGG               CCC^GGG
             2822  CCCGGG              
SmlI         2397  CTCGAG               C^T[CT][GA]AG
             3702  CTTAAG              
StuI         1968  AGGCCT               AGG^CCT
             3561  AGGCCT              
TaqI         2398  TCGA                 T^CGA
             2801  TCGA                
Tsp509I       954  AATT                 ^AATT
             3727  AATT                
TthHB8I      2398  TCGA                 T^CGA
             2801  TCGA                
Van91I       2137  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3037  CCAGGGTATGG         
XmaCI         909  CCCGGG               C^CCGGG
             2822  CCCGGG              
XmaI          909  CCCGGG               C^CCGGG
             2822  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp45I      TspRI       
XcmI        XhoII