##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BglII BlnI BsaBI
BsiWI BspDI BspHI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT22I KpnI MamI MfeI
MluI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RcaI RsrII SbfI SfuI SnaBI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 469 TCCGGA T^CCGGA
AclI 3921 AACGTT AA^CGTT
AflII 3366 CTTAAG C^TTAAG
Asp700I 4227 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 922 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3625 TGCGCA TGC^GCA
BbrPI 2166 CACGTG CAC^GTG
BciVI 2090 GTATCC GTATCC(6/5)
BfrI 3366 CTTAAG C^TTAAG
BsaAI 2166 CACGTG [CT]AC^GT[GA]
BseAI 469 TCCGGA T^CCGGA
BsgI 2412 GTGCAG GTGCAG(16/14)
BsmI 2267 GAATGC GAATGC(1/-1)
BspEI 469 TCCGGA T^CCGGA
BssHII 2284 GCGCGC G^CGCGC
DraI 3149 TTTAAA TTT^AAA
DraIII 2514 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1547 CGGCCG C^GGCCG
EclXI 1547 CGGCCG C^GGCCG
Eco52I 1547 CGGCCG C^GGCCG
FseI 2171 GGCCGGCC GGCCGG^CC
FspI 3625 TGCGCA TGC^GCA
HindIII 3359 AAGCTT A^AGCTT
HpaI 2903 GTTAAC GTT^AAC
MroI 469 TCCGGA T^CCGGA
PflFI 922 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2166 CACGTG CAC^GTG
PmlI 2166 CACGTG CAC^GTG
PpuMI 968 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 233 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3921 AACGTT AA^CGTT
SacI 350 GAGCTC GAGCT^C
ScaI 1632 AGTACT AGT^ACT
SfiI 1073 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2210 CACCGGCG C[GA]^CCGG[CT]G
SmlI 3366 CTTAAG C^T[CT][GA]AG
StuI 619 AGGCCT AGG^CCT
Tth111I 922 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4227 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1143 GACGTC GACGT^C
1164 GACGTC
AcsI 78 GAATTT [GA]^AATT[CT]
4026 AAATTT
Aor51HI 506 AGCGCT AGC^GCT
3533 AGCGCT
ApoI 78 GAATTT [GA]^AATT[CT]
4026 AAATTT
BalI 1081 TGGCCA TGG^CCA
2923 TGGCCA
BbsI 2967 GAAGAC GAAGAC(2/6)
2303 GTCTTC
BbvCI 2094 CCTCAGC CCTCAGC(-5/-2)
3349 CCTCAGC
BcgI 2377 CGACTGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4166 CGATTCCTCTGC
BclI 1507 TGATCA T^GATCA
3233 TGATCA
BpmI 1000 CTCCAG CTGGAG(16/14)
2380 CTGGAG
BpuAI 2967 GAAGAC GAAGAC(2/6)
2303 GTCTTC
BsaI 231 GAGACC GGTCTC(1/5)
810 GAGACC
BsiEI 1547 CGGCCG CG[GA][CT]^CG
1912 CGACCG
BspLU11I 334 ACATGT A^CATGT
1485 ACATGT
BspMI 2155 ACCTGC ACCTGC(4/8)
2357 GCAGGT
BstAPI 3603 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4391 GCATGATCTGC
BstXI 1369 CCAGTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2678 CCAGCATGTTGG
BstYI 1605 AGATCC [GA]^GATC[CT]
3098 GGATCT
DrdI 1587 GACAGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1754 GACTGCTGTGTC
Eco47III 506 AGCGCT AGC^GCT
3533 AGCGCT
FbaI 1507 TGATCA T^GATCA
3233 TGATCA
MflI 1605 AGATCC [GA]^GATC[CT]
3098 GGATCT
MluNI 1081 TGGCCA TGG^CCA
2923 TGGCCA
MscI 1081 TGGCCA TGG^CCA
2923 TGGCCA
NcoI 2323 CCATGG C^CATGG
3680 CCATGG
PflMI 859 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3451 CCATGCTTTGG
PvuII 1293 CAGCTG CAG^CTG
2634 CAGCTG
SalI 330 GTCGAC G^TCGAC
2787 GTCGAC
SapI 1040 GAAGAGC GCTCTTC(1/4)
1213 GCTCTTC
SexAI 2501 ACCTGGT A^CC[AT]GGT
3619 ACCTGGT
SpeI 4469 ACTAGT A^CTAGT
4542 ACTAGT
Van91I 859 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3451 CCATGCTTTGG
XhoII 1605 AGATCC [GA]^GATC[CT]
3098 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BglI BlpI BmyI Bpu1102I BsaHI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI