##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccII       AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      ApaLI       AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BamHI       BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BlnI        BpuAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HpaI        KspI        
MfeI        MluI        MunI        MvnI        NaeI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Van91I      XcmI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Acc65I       3886  GGTACC               G^GTACC
AccI         1711  GTAGAC               GT^[AC][GT]AC
AccIII       2796  TCCGGA               T^CCGGA
Aor51HI      1043  AGCGCT               AGC^GCT
Asp718I      3886  GGTACC               G^GTACC
BbeI         3853  GGCGCC               GGCGC^C
BbvCI         573  GCTGAGG              CCTCAGC(-5/-2)
BsaAI        1687  CACGTA               [CT]AC^GT[GA]
BsaWI        2796  TCCGGA               [AT]^CCGG[AT]
BseAI        2796  TCCGGA               T^CCGGA
BsmI          195  GCATTC               GAATGC(1/-1)
BspEI        2796  TCCGGA               T^CCGGA
BsrFI        1573  GCCGGT               [GA]^CCGG[CT]
BssSI        1705  CTCGTG               CACGAG(-5/-1)
BstAPI       4313  GCAGCCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I       1573  GCCGGT               [GA]^CCGG[CT]
Eco47III     1043  AGCGCT               AGC^GCT
EcoNI         935  CCTTGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2119  GAATTC               G^AATTC
HindIII       328  AAGCTT               A^AGCTT
KasI         3853  GGCGCC               G^GCGCC
KpnI         3886  GGTACC               GGTAC^C
MroI         2796  TCCGGA               T^CCGGA
NarI         3853  GGCGCC               GG^CGCC
NheI         3859  GCTAGC               G^CTAGC
NspI         1988  GCATGT               [GA]CATG^[CT]
PaeR7I        304  CTCGAG               C^TCGAG
SfiI         3560  GGCCGAACGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         3853  GGCGCC               GGC^GCC
XbaI         1602  TCTAGA               T^CTAGA
XhoI          304  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI         1957  GGCGTC               G[GA]^CG[CT]C
             3853  GGCGCC              
AocI          363  CCTGAGG              CC^T[ACGT]AGG
              611  CCTAAGG             
ApaI         3354  GGGCCC               GGGCC^C
             4281  GGGCCC              
BglI         2239  GCCCGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3561  GCCGAACGGGC         
BlpI          428  GCTCAGC              GC^T[ACGT]AGC
             1413  GCTCAGC             
Bpu1102I      428  GCTCAGC              GC^T[ACGT]AGC
             1413  GCTCAGC             
BsaBI        1606  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1732  GATCAACATC          
BsaHI        1957  GGCGTC               G[GA]^CG[CT]C
             3853  GGCGCC              
BsgI         3042  CTGCAC               GTGCAG(16/14)
             3186  CTGCAC              
BsrDI        2988  CATTGC               GCAATG(2/0)
             2227  GCAATG              
BstEII         65  GGTGACC              G^GT[ACGT]ACC
             4240  GGTCACC             
BstPI          65  GGTGACC              G^GT[ACGT]ACC
             4240  GGTCACC             
Bsu36I        363  CCTGAGG              CC^T[ACGT]AGG
              611  CCTAAGG             
CelII         428  GCTCAGC              GC^T[ACGT]AGC
             1413  GCTCAGC             
Eco81I        363  CCTGAGG              CC^T[ACGT]AGG
              611  CCTAAGG             
EcoO65I        65  GGTGACC              G^GT[ACGT]ACC
             4240  GGTCACC             
Hin1I        1957  GGCGTC               G[GA]^CG[CT]C
             3853  GGCGCC              
MamI         1606  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1732  GATCAACATC          
MslI         2508  CATGCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3830  CACACCTGTG          
NcoI         1156  CCATGG               C^CATGG
             2155  CCATGG              
PpuMI        2562  GGGACCC              [GA]G^G[AT]CC[CT]
             3372  AGGTCCC             
SacI          426  GAGCTC               GAGCT^C
             2276  GAGCTC              
SapI         1510  GAAGAGC              GCTCTTC(1/4)
             3609  GAAGAGC             
SmaI         2854  CCCGGG               CCC^GGG
             3229  CCCGGG              
SmlI          304  CTCGAG               C^T[CT][GA]AG
              936  CTTGAG              
XmaCI        2854  CCCGGG               C^CCGGG
             3229  CCCGGG              
XmaI         2854  CCCGGG               C^CCGGG
             3229  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflFI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XhoII       
XmnI