################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfrI BlnI BpuAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KspI MfeI MluI MunI MvnI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XcmI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 3886 GGTACC G^GTACC AccI 1711 GTAGAC GT^[AC][GT]AC AccIII 2796 TCCGGA T^CCGGA Aor51HI 1043 AGCGCT AGC^GCT Asp718I 3886 GGTACC G^GTACC BbeI 3853 GGCGCC GGCGC^C BbvCI 573 GCTGAGG CCTCAGC(-5/-2) BsaAI 1687 CACGTA [CT]AC^GT[GA] BsaWI 2796 TCCGGA [AT]^CCGG[AT] BseAI 2796 TCCGGA T^CCGGA BsmI 195 GCATTC GAATGC(1/-1) BspEI 2796 TCCGGA T^CCGGA BsrFI 1573 GCCGGT [GA]^CCGG[CT] BssSI 1705 CTCGTG CACGAG(-5/-1) BstAPI 4313 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1573 GCCGGT [GA]^CCGG[CT] Eco47III 1043 AGCGCT AGC^GCT EcoNI 935 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2119 GAATTC G^AATTC HindIII 328 AAGCTT A^AGCTT KasI 3853 GGCGCC G^GCGCC KpnI 3886 GGTACC GGTAC^C MroI 2796 TCCGGA T^CCGGA NarI 3853 GGCGCC GG^CGCC NheI 3859 GCTAGC G^CTAGC NspI 1988 GCATGT [GA]CATG^[CT] PaeR7I 304 CTCGAG C^TCGAG SfiI 3560 GGCCGAACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3853 GGCGCC GGC^GCC XbaI 1602 TCTAGA T^CTAGA XhoI 304 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 1957 GGCGTC G[GA]^CG[CT]C 3853 GGCGCC AocI 363 CCTGAGG CC^T[ACGT]AGG 611 CCTAAGG ApaI 3354 GGGCCC GGGCC^C 4281 GGGCCC BglI 2239 GCCCGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3561 GCCGAACGGGC BlpI 428 GCTCAGC GC^T[ACGT]AGC 1413 GCTCAGC Bpu1102I 428 GCTCAGC GC^T[ACGT]AGC 1413 GCTCAGC BsaBI 1606 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1732 GATCAACATC BsaHI 1957 GGCGTC G[GA]^CG[CT]C 3853 GGCGCC BsgI 3042 CTGCAC GTGCAG(16/14) 3186 CTGCAC BsrDI 2988 CATTGC GCAATG(2/0) 2227 GCAATG BstEII 65 GGTGACC G^GT[ACGT]ACC 4240 GGTCACC BstPI 65 GGTGACC G^GT[ACGT]ACC 4240 GGTCACC Bsu36I 363 CCTGAGG CC^T[ACGT]AGG 611 CCTAAGG CelII 428 GCTCAGC GC^T[ACGT]AGC 1413 GCTCAGC Eco81I 363 CCTGAGG CC^T[ACGT]AGG 611 CCTAAGG EcoO65I 65 GGTGACC G^GT[ACGT]ACC 4240 GGTCACC Hin1I 1957 GGCGTC G[GA]^CG[CT]C 3853 GGCGCC MamI 1606 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1732 GATCAACATC MslI 2508 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3830 CACACCTGTG NcoI 1156 CCATGG C^CATGG 2155 CCATGG PpuMI 2562 GGGACCC [GA]G^G[AT]CC[CT] 3372 AGGTCCC SacI 426 GAGCTC GAGCT^C 2276 GAGCTC SapI 1510 GAAGAGC GCTCTTC(1/4) 3609 GAAGAGC SmaI 2854 CCCGGG CCC^GGG 3229 CCCGGG SmlI 304 CTCGAG C^T[CT][GA]AG 936 CTTGAG XmaCI 2854 CCCGGG C^CCGGG 3229 CCCGGG XmaI 2854 CCCGGG C^CCGGG 3229 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglII BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmnI