##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AclI AcsI ApoI AscI
AseI AsnI Asp700I AviII AvrII
BciVI BclI BlnI BseAI BsiWI
BsmBI BspDI BspEI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EcoRI EcoRV FbaI FspI HindIII
HpaI MfeI MluI MroI MunI
NdeI NotI NruI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SfuI
SnaBI SpeI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 640 GGTACC G^GTACC
AflII 2351 CTTAAG C^TTAAG
AgeI 2470 ACCGGT A^CCGGT
AhdI 2633 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3253 CCTTAGG CC^T[ACGT]AGG
Asp718I 640 GGTACC G^GTACC
AspEI 2633 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2759 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 603 GGATCC G^GATCC
BfrI 2351 CTTAAG C^TTAAG
BglII 1904 AGATCT A^GATCT
BsiEI 1691 CGGCCG CG[GA][CT]^CG
BsmI 2853 GAATGC GAATGC(1/-1)
Bsp1407I 4007 TGTACA T^GTACA
BspLU11I 2484 ACATGT A^CATGT
BsrGI 4007 TGTACA T^GTACA
BstXI 2491 CCACCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3253 CCTTAGG CC^T[ACGT]AGG
DraI 4066 TTTAAA TTT^AAA
EagI 1691 CGGCCG C^GGCCG
Eam1105I 2633 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1691 CGGCCG C^GGCCG
Eco52I 1691 CGGCCG C^GGCCG
Eco81I 3253 CCTTAGG CC^T[ACGT]AGG
EcoT22I 520 ATGCAT ATGCA^T
HincII 3404 GTCAAC GT[CT]^[GA]AC
HindII 3404 GTCAAC GT[CT]^[GA]AC
KpnI 640 GGTACC GGTAC^C
KspI 899 CCGCGG CCGC^GG
NheI 320 GCTAGC G^CTAGC
NsiI 520 ATGCAT ATGCA^T
PflFI 2759 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2470 ACCGGT A^CCGGT
SacII 899 CCGCGG CCGC^GG
SapI 137 GAAGAGC GCTCTTC(1/4)
SfiI 961 GGCCTCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2469 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2930 CCCGGG CCC^GGG
SspBI 4007 TGTACA T^GTACA
SspI 4037 AATATT AAT^ATT
Tth111I 2759 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2930 CCCGGG C^CCGGG
XmaI 2930 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 239 GTGCAC G^TGCAC
3773 GTGCAC
Aor51HI 1229 AGCGCT AGC^GCT
3801 AGCGCT
ApaLI 239 GTGCAC G^TGCAC
3773 GTGCAC
BbeI 222 GGCGCC GGCGC^C
1457 GGCGCC
BbrPI 1985 CACGTG CAC^GTG
2750 CACGTG
BcgI 2086 CGAGTCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2706 GCAAGCCCATCG
BfaI 321 CTAG C^TAG
3552 CTAG
BsaBI 3577 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3583 GATCTACATC
BstAPI 1600 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3226 GCAGGACCTGC
Eco47III 1229 AGCGCT AGC^GCT
3801 AGCGCT
FseI 1438 GGCCGGCC GGCCGG^CC
1688 GGCCGGCC
KasI 222 GGCGCC G^GCGCC
1457 GGCGCC
MaeI 321 CTAG C^TAG
3552 CTAG
MamI 3577 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3583 GATCTACATC
NarI 222 GGCGCC GG^CGCC
1457 GGCGCC
PaeR7I 828 CTCGAG C^TCGAG
1147 CTCGAG
PmaCI 1985 CACGTG CAC^GTG
2750 CACGTG
PmlI 1985 CACGTG CAC^GTG
2750 CACGTG
SexAI 2760 ACCAGGT A^CC[AT]GGT
3285 ACCTGGT
SfoI 222 GGCGCC GGC^GCC
1457 GGCGCC
XhoI 828 CTCGAG C^TCGAG
1147 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI SphI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII