##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AspI AvrII
BbeI BbrPI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspEI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
Cfr10I CpoI DrdI Eam1105I Eco47III
FbaI FseI Hin1I HpaI KasI
MamI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 210 GGTACC G^GTACC
ApaI 117 GGGCCC GGGCC^C
Asp718I 210 GGTACC G^GTACC
AspHI 37 GTGCTC G[AT]GC[AT]^C
AviII 26 TGCGCA TGC^GCA
BamHI 57 GGATCC G^GATCC
BanI 210 GGTACC G^G[CT][GA]CC
BbsI 1327 GAAGAC GAAGAC(2/6)
BcgI 17 CGAGGCGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3044 GTATCC GTATCC(6/5)
BglII 3345 AGATCT A^GATCT
BpuAI 1327 GAAGAC GAAGAC(2/6)
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
BspDI 643 ATCGAT AT^CGAT
BspLU11I 3524 ACATGT A^CATGT
BsrDI 2849 CATTGC GCAATG(2/0)
BssSI 3720 CTCGTG CACGAG(-5/-1)
BstEII 2653 GGTCACC G^GT[ACGT]ACC
BstPI 2653 GGTCACC G^GT[ACGT]ACC
BstXI 780 CCAAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 643 ATCGAT AT^CGAT
DraIII 3795 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2653 GGTCACC G^GT[ACGT]ACC
EcoRV 1495 GATATC GAT^ATC
EcoT22I 3375 ATGCAT ATGCA^T
FspI 26 TGCGCA TGC^GCA
HincII 600 GTCAAC GT[CT]^[GA]AC
HindII 600 GTCAAC GT[CT]^[GA]AC
KpnI 210 GGTACC GGTAC^C
MaeII 1488 ACGT A^CGT
MluI 100 ACGCGT A^CGCGT
NruI 185 TCGCGA TCG^CGA
NsiI 3375 ATGCAT ATGCA^T
PflMI 1627 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1 GAAGAGC GCTCTTC(1/4)
SmaI 203 CCCGGG CCC^GGG
SpeI 994 ACTAGT A^CTAGT
SspI 90 AATATT AAT^ATT
Van91I 1627 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3766 TCTAGA T^CTAGA
XmaCI 203 CCCGGG C^CCGGG
XmaI 203 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 100 ACGCGT A^C[GA][CT]GT
3524 ACATGT
AocI 1180 CCTGAGG CC^T[ACGT]AGG
3190 CCTGAGG
AseI 1825 ATTAAT AT^TAAT
3893 ATTAAT
AsnI 1825 ATTAAT AT^TAAT
3893 ATTAAT
BalI 623 TGGCCA TGG^CCA
3734 TGGCCA
BbvCI 661 CCTCAGC CCTCAGC(-5/-2)
3699 GCTGAGG
BglI 337 GCCCCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
370 GCCGCCGCGGC
BsgI 3107 CTGCAC GTGCAG(16/14)
424 GTGCAG
BsmI 1523 GAATGC GAATGC(1/-1)
3624 GCATTC
BspHI 467 TCATGA T^CATGA
3533 TCATGA
BspMI 2793 ACCTGC ACCTGC(4/8)
3818 GCAGGT
BstAPI 1645 GCAATTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2788 GCAGCACCTGC
Bsu36I 1180 CCTGAGG CC^T[ACGT]AGG
3190 CCTGAGG
EagI 152 CGGCCG C^GGCCG
216 CGGCCG
EclXI 152 CGGCCG C^GGCCG
216 CGGCCG
Eco52I 152 CGGCCG C^GGCCG
216 CGGCCG
Eco81I 1180 CCTGAGG CC^T[ACGT]AGG
3190 CCTGAGG
EcoRI 1109 GAATTC G^AATTC
1680 GAATTC
HgaI 161 GACGC GACGC(5/10)
102 GCGTC
HindIII 3655 AAGCTT A^AGCTT
4128 AAGCTT
MfeI 1646 CAATTG C^AATTG
2828 CAATTG
MluNI 623 TGGCCA TGG^CCA
3734 TGGCCA
MscI 623 TGGCCA TGG^CCA
3734 TGGCCA
MunI 1646 CAATTG C^AATTG
2828 CAATTG
NspI 3488 GCATGT [GA]CATG^[CT]
3524 ACATGT
PpuMI 249 GGGTCCT [GA]G^G[AT]CC[CT]
275 GGGACCC
PshBI 1825 ATTAAT AT^TAAT
3893 ATTAAT
PvuII 485 CAGCTG CAG^CTG
3142 CAGCTG
RcaI 467 TCATGA T^CATGA
3533 TCATGA
SmlI 1514 CTCAAG C^T[CT][GA]AG
3853 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI