################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI BamHI BbeI BbrPI BclI BfrI BsaAI BsaBI BseAI BsiEI BsiWI Bsp1407I BspEI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI HaeII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI PacI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 958 CGCG CG^CG AcyI 3638 GACGCC G[GA]^CG[CT]C AflIII 4175 ACATGT A^C[GA][CT]GT AgeI 3439 ACCGGT A^CCGGT ApaI 3160 GGGCCC GGGCC^C Asp700I 2903 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1257 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3189 CCTAGG C^CTAGG BciVI 3199 GTATCC GTATCC(6/5) BglII 2085 AGATCT A^GATCT BlnI 3189 CCTAGG C^CTAGG BsaHI 3638 GACGCC G[GA]^CG[CT]C BsmBI 974 CGTCTC CGTCTC(1/5) BspDI 293 ATCGAT AT^CGAT BspLU11I 4175 ACATGT A^CATGT BstBI 1601 TTCGAA TT^CGAA BstUI 958 CGCG CG^CG ClaI 293 ATCGAT AT^CGAT DraI 3559 TTTAAA TTT^AAA DraIII 3790 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2388 GACTCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 287 ATGCAT ATGCA^T HgaI 3638 GACGC GACGC(5/10) Hin1I 3638 GACGCC G[GA]^CG[CT]C MvnI 958 CGCG CG^CG NaeI 1284 GCCGGC GCC^GGC NgoMIV 1284 GCCGGC G^CCGGC NsiI 287 ATGCAT ATGCA^T NspV 1601 TTCGAA TT^CGAA PaeR7I 2390 CTCGAG C^TCGAG PflFI 1257 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3439 ACCGGT A^CCGGT SacI 2131 GAGCTC GAGCT^C SfuI 1601 TTCGAA TT^CGAA SphI 1941 GCATGC GCATG^C SspI 3824 AATATT AAT^ATT Tth111I 1257 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2390 CTCGAG C^TCGAG XmnI 2903 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AvaI 2390 CTCGAG C^[CT]CG[GA]G 3349 CCCGAG AviII 1992 TGCGCA TGC^GCA 2527 TGCGCA BalI 141 TGGCCA TGG^CCA 3848 TGGCCA BlpI 2440 GCTGAGC GC^T[ACGT]AGC 3250 GCTAAGC Bpu1102I 2440 GCTGAGC GC^T[ACGT]AGC 3250 GCTAAGC BsaI 1998 GGTCTC GGTCTC(1/5) 4098 GGTCTC BsaWI 2186 ACCGGA [AT]^CCGG[AT] 3439 ACCGGT BsgI 2113 GTGCAG GTGCAG(16/14) 4143 GTGCAG BsmI 776 GAATGC GAATGC(1/-1) 3811 GCATTC BsoBI 2390 CTCGAG C^[CT]CG[GA]G 3349 CCCGAG BsrDI 549 CATTGC GCAATG(2/0) 43 GCAATG BstAPI 280 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1351 GCACCTTCTGC BstYI 1784 AGATCC [GA]^GATC[CT] 2085 AGATCT CelII 2440 GCTGAGC GC^T[ACGT]AGC 3250 GCTAAGC CfoI 1993 GCGC GCG^C 2528 GCGC EcoNI 184 CCTAAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2217 CCTGGAGGAGG FspI 1992 TGCGCA TGC^GCA 2527 TGCGCA HhaI 1993 GCGC GCG^C 2528 GCGC HinP1I 1993 GCGC G^CGC 2528 GCGC HincII 392 GTCAAC GT[CT]^[GA]AC 4148 GTCAAC HindII 392 GTCAAC GT[CT]^[GA]AC 4148 GTCAAC MflI 1784 AGATCC [GA]^GATC[CT] 2085 AGATCT MluNI 141 TGGCCA TGG^CCA 3848 TGGCCA MscI 141 TGGCCA TGG^CCA 3848 TGGCCA PpuMI 596 AGGACCT [GA]G^G[AT]CC[CT] 3359 GGGACCC SapI 2890 GAAGAGC GCTCTTC(1/4) 1060 GCTCTTC SexAI 2720 ACCAGGT A^CC[AT]GGT 3878 ACCTGGT XhoII 1784 AGATCC [GA]^GATC[CT] 2085 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I