##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BfrI
BlnI BsaAI BseAI BsiWI BspEI
BspHI BspMI BsrDI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HindIII HpaI KpnI KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 2120 GGCGCC G[GA]^CG[CT]C
AflIII 308 ACATGT A^C[GA][CT]GT
BalI 625 TGGCCA TGG^CCA
BbeI 2120 GGCGCC GGCGC^C
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BclI 1955 TGATCA T^GATCA
BglI 3057 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3451 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3451 GCTCAGC GC^T[ACGT]AGC
BsaBI 1872 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2120 GGCGCC G[GA]^CG[CT]C
BsiEI 1515 CGGTCG CG[GA][CT]^CG
BsmI 1208 GCATTC GAATGC(1/-1)
Bsp1407I 3746 TGTACA T^GTACA
BspDI 3167 ATCGAT AT^CGAT
BspLU11I 308 ACATGT A^CATGT
BsrGI 3746 TGTACA T^GTACA
BstBI 248 TTCGAA TT^CGAA
BstXI 128 CCAGTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3451 GCTCAGC GC^T[ACGT]AGC
ClaI 3167 ATCGAT AT^CGAT
EcoNI 3248 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1756 ATGCAT ATGCA^T
FbaI 1955 TGATCA T^GATCA
Hin1I 2120 GGCGCC G[GA]^CG[CT]C
HincII 2714 GTCAAC GT[CT]^[GA]AC
HindII 2714 GTCAAC GT[CT]^[GA]AC
KasI 2120 GGCGCC G^GCGCC
MamI 1872 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 625 TGGCCA TGG^CCA
MscI 625 TGGCCA TGG^CCA
NarI 2120 GGCGCC GG^CGCC
NcoI 1899 CCATGG C^CATGG
NsiI 1756 ATGCAT ATGCA^T
NspV 248 TTCGAA TT^CGAA
PpuMI 2925 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 1765 GAGCTC GAGCT^C
SexAI 2253 ACCTGGT A^CC[AT]GGT
SfoI 2120 GGCGCC GGC^GCC
SfuI 248 TTCGAA TT^CGAA
SmaI 1717 CCCGGG CCC^GGG
SspBI 3746 TGTACA T^GTACA
SspI 4195 AATATT AAT^ATT
XbaI 1449 TCTAGA T^CTAGA
XcmI 3049 CCAGAGAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1717 CCCGGG C^CCGGG
XmaI 1717 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2755 GTAGAC GT^[AC][GT]AC
2867 GTAGAC
AgeI 2831 ACCGGT A^CCGGT
3105 ACCGGT
AocI 1173 CCTCAGG CC^T[ACGT]AGG
3418 CCTCAGG
AseI 800 ATTAAT AT^TAAT
1701 ATTAAT
AsnI 800 ATTAAT AT^TAAT
1701 ATTAAT
Asp700I 197 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1387 GAACACATTC
AspHI 1765 GAGCTC G[AT]GC[AT]^C
4062 GAGCAC
BanI 2120 GGCGCC G^G[CT][GA]CC
2583 GGCACC
BciVI 2783 GGATAC GTATCC(6/5)
2519 GTATCC
BglII 763 AGATCT A^GATCT
1587 AGATCT
BsaI 3029 GAGACC GGTCTC(1/5)
4111 GGTCTC
BsgI 131 GTGCAG GTGCAG(16/14)
2332 GTGCAG
BsiHKAI 1765 GAGCTC G[AT]GC[AT]^C
4062 GAGCAC
BsmBI 111 CGTCTC CGTCTC(1/5)
2013 GAGACG
Bsu36I 1173 CCTCAGG CC^T[ACGT]AGG
3418 CCTCAGG
DraI 1832 TTTAAA TTT^AAA
4079 TTTAAA
Eco81I 1173 CCTCAGG CC^T[ACGT]AGG
3418 CCTCAGG
EcoRI 2618 GAATTC G^AATTC
3329 GAATTC
HaeII 78 AGCGCC [GA]GCGC^[CT]
2120 GGCGCC
HgaI 110 GCGTC GACGC(5/10)
1215 GCGTC
MslI 638 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1700 CATTAATATG
NspI 308 ACATGT [GA]CATG^[CT]
3447 ACATGC
PinAI 2831 ACCGGT A^CCGGT
3105 ACCGGT
PshAI 2057 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2557 GACATTCGTC
PshBI 800 ATTAAT AT^TAAT
1701 ATTAAT
SapI 1767 GCTCTTC GCTCTTC(1/4)
2665 GCTCTTC
ScaI 521 AGTACT AGT^ACT
1611 AGTACT
XmnI 197 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1387 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII