##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp700I
AviII AvrII BbrPI BcgI BclI
BfrI BlnI BsaAI BseAI BsiWI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HpaI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI ScaI SfuI
SnaBI SpeI SspBI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 829 GACGTC GACGT^C
AccI 31 GTAGAC GT^[AC][GT]AC
AflIII 4112 ACGTGT A^C[GA][CT]GT
AhdI 824 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 824 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3006 GGATCC G^GATCC
BciVI 2903 GTATCC GTATCC(6/5)
BfaI 3827 CTAG C^TAG
BsaBI 1473 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1292 ACCGGA [AT]^CCGG[AT]
BsmI 2948 GCATTC GAATGC(1/-1)
BspDI 985 ATCGAT AT^CGAT
BsrDI 942 CATTGC GCAATG(2/0)
ClaI 985 ATCGAT AT^CGAT
CpoI 3508 CGGTCCG CG^G[AT]CCG
DraI 4874 TTTAAA TTT^AAA
Eam1105I 824 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 3409 GGCCGGCC GGCCGG^CC
KspI 3218 CCGCGG CCGC^GG
MaeI 3827 CTAG C^TAG
MamI 1473 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 4613 CTCGAG C^TCGAG
PshAI 75 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3508 CGGTCCG CG^G[AT]CCG
SacII 3218 CCGCGG CCGC^GG
SbfI 2754 CCTGCAGG CCTGCA^GG
SfiI 4609 GGCCCTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1842 CACCGGCG C[GA]^CCGG[CT]G
SphI 1388 GCATGC GCATG^C
Sse8387I 2754 CCTGCAGG CCTGCA^GG
TfiI 781 GAATC G^A[AT]TC
Tsp509I 679 AATT ^AATT
XhoI 4613 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 2045 GGTACC G^GTACC
4519 GGTACC
Asp718I 2045 GGTACC G^GTACC
4519 GGTACC
AspI 586 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3018 GACCGTGTC
BbeI 1846 GGCGCC GGCGC^C
3000 GGCGCC
BglII 1529 AGATCT A^GATCT
3539 AGATCT
BlpI 1303 GCTCAGC GC^T[ACGT]AGC
1356 GCTGAGC
Bpu1102I 1303 GCTCAGC GC^T[ACGT]AGC
1356 GCTGAGC
BssSI 871 CACGAG CACGAG(-5/-1)
1393 CACGAG
BstEII 1288 GGTCACC G^GT[ACGT]ACC
2414 GGTCACC
BstPI 1288 GGTCACC G^GT[ACGT]ACC
2414 GGTCACC
CelII 1303 GCTCAGC GC^T[ACGT]AGC
1356 GCTGAGC
DrdI 1012 GACAGCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2377 GACCTGCATGTC
EcoO65I 1288 GGTCACC G^GT[ACGT]ACC
2414 GGTCACC
HindIII 4901 AAGCTT A^AGCTT
4916 AAGCTT
KasI 1846 GGCGCC G^GCGCC
3000 GGCGCC
KpnI 2045 GGTACC GGTAC^C
4519 GGTACC
MseI 4875 TTAA T^TAA
4898 TTAA
NarI 1846 GGCGCC GG^CGCC
3000 GGCGCC
PflFI 586 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3018 GACCGTGTC
SexAI 839 ACCAGGT A^CC[AT]GGT
4306 ACCTGGT
SfoI 1846 GGCGCC GGC^GCC
3000 GGCGCC
SmlI 1972 CTCAAG C^T[CT][GA]AG
4613 CTCGAG
StuI 3821 AGGCCT AGG^CCT
3928 AGGCCT
Tru9I 4875 TTAA T^TAA
4898 TTAA
Tth111I 586 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3018 GACCGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI