##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     BamHI       BfrI        BsiWI       
BsmI        BspDI       BspHI       Bst1107I    BstZ17I     
ClaI        CpoI        Eco47III    EcoRI       EcoT22I     
HindIII     HpaI        KpnI        KspI        MfeI        
MunI        NdeI        NotI        NruI        NsiI        
PacI        PaeR7I      PinAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SgrAI       
SnaBI       SpeI        SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII       3022  TCCGGA               T^CCGGA
AcsI         2354  AAATTC               [GA]^AATT[CT]
AhdI         3485  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         4001  GGGCCC               GGGCC^C
ApoI         2354  AAATTC               [GA]^AATT[CT]
AspEI        3485  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         830  CCTAGG               C^CTAGG
BbrPI        4359  CACGTG               CAC^GTG
BciVI        1560  GTATCC               GTATCC(6/5)
BclI         2730  TGATCA               T^GATCA
BlnI          830  CCTAGG               C^CTAGG
BsaAI        4359  CACGTG               [CT]AC^GT[GA]
BseAI        3022  TCCGGA               T^CCGGA
BsiEI        2569  CGGCCG               CG[GA][CT]^CG
Bsp1407I     4355  TGTACA               T^GTACA
BspEI        3022  TCCGGA               T^CCGGA
BsrGI        4355  TGTACA               T^GTACA
BssHII       3593  GCGCGC               G^CGCGC
BstAPI       4058  GCACCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1081  GGTGACC              G^GT[ACGT]ACC
BstPI        1081  GGTGACC              G^GT[ACGT]ACC
DrdI          236  GACTCCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2569  CGGCCG               C^GGCCG
Eam1105I     3485  GACGACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2569  CGGCCG               C^GGCCG
Eco52I       2569  CGGCCG               C^GGCCG
EcoO65I      1081  GGTGACC              G^GT[ACGT]ACC
EcoRV         353  GATATC               GAT^ATC
FbaI         2730  TGATCA               T^GATCA
FseI         2566  GGCCGGCC             GGCCGG^CC
MluI          644  ACGCGT               A^CGCGT
MroI         3022  TCCGGA               T^CCGGA
NheI         2534  GCTAGC               G^CTAGC
PmaCI        4359  CACGTG               CAC^GTG
PmeI         2395  GTTTAAAC             GTTT^AAAC
PmlI         4359  CACGTG               CAC^GTG
PshAI        1084  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         2134  CCTGCAGG             CCTGCA^GG
ScaI         2541  AGTACT               AGT^ACT
SphI         1902  GCATGC               GCATG^C
Sse8387I     2134  CCTGCAGG             CCTGCA^GG
SspBI        4355  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

Asp700I      1405  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3232  GAAGAAGTTC          
AspI          325  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3661  GACGGAGTC           
AviII        1369  TGCGCA               TGC^GCA
             4339  TGCGCA              
BbeI         1378  GGCGCC               GGCGC^C
             3985  GGCGCC              
BbvCI        1345  CCTCAGC              CCTCAGC(-5/-2)
             2062  CCTCAGC             
BcgI         1723  CGAGCACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3034  GCACTACCTTCG        
BglII        2917  AGATCT               A^GATCT
             4093  AGATCT              
BsaBI        1003  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2918  GATCTGGATC          
BsaWI        2607  ACCGGA               [AT]^CCGG[AT]
             3022  TCCGGA              
BsmBI         426  GAGACG               CGTCTC(1/5)
             3077  GAGACG              
BsrDI        1820  GCAATG               GCAATG(2/0)
             3532  GCAATG              
BsrFI        2567  GCCGGC               [GA]^CCGG[CT]
             3988  GCCGGC              
BstBI        1961  TTCGAA               TT^CGAA
             3042  TTCGAA              
Cfr10I       2567  GCCGGC               [GA]^CCGG[CT]
             3988  GCCGGC              
EcoNI        1072  CCTGAGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4290  CCTGACTGAGG         
FspI         1369  TGCGCA               TGC^GCA
             4339  TGCGCA              
KasI         1378  GGCGCC               G^GCGCC
             3985  GGCGCC              
MamI         1003  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2918  GATCTGGATC          
NaeI         2567  GCCGGC               GCC^GGC
             3988  GCCGGC              
NarI         1378  GGCGCC               GG^CGCC
             3985  GGCGCC              
NcoI         1428  CCATGG               C^CATGG
             3162  CCATGG              
NgoMIV       2567  GCCGGC               G^CCGGC
             3988  GCCGGC              
NspV         1961  TTCGAA               TT^CGAA
             3042  TTCGAA              
PflFI         325  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3661  GACGGAGTC           
PflMI        2419  CCAGTCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3409  CCAGGTGGTGG         
SalI         2099  GTCGAC               G^TCGAC
             3611  GTCGAC              
SexAI        4038  ACCTGGT              A^CC[AT]GGT
             4215  ACCAGGT             
SfiI          769  GGCCAACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              834  GGCCCATCCGGCC       
SfoI         1378  GGCGCC               GGC^GCC
             3985  GGCGCC              
SfuI         1961  TTCGAA               TT^CGAA
             3042  TTCGAA              
SmaI          437  CCCGGG               CCC^GGG
             1631  CCCGGG              
Tth111I       325  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3661  GACGGAGTC           
Van91I       2419  CCAGTCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3409  CCAGGTGGTGG         
XmaCI         437  CCCGGG               C^CCGGG
             1631  CCCGGG              
XmaI          437  CCCGGG               C^CCGGG
             1631  CCCGGG              
XmnI         1405  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3232  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspLU11I    BspMI       
BsrBI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII