##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AvaI
AviII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HgaI Hin1I KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 422 GTGCAC G^TGCAC
AlwNI 2438 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 422 GTGCAC G^TGCAC
AspI 971 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 413 GAGCTC G[GA]GC[CT]^C
BlpI 1759 GCTGAGC GC^T[ACGT]AGC
BpmI 2003 CTCCAG CTGGAG(16/14)
Bpu1102I 1759 GCTGAGC GC^T[ACGT]AGC
BsaWI 2101 TCCGGT [AT]^CCGG[AT]
BsmBI 2237 GAGACG CGTCTC(1/5)
BsmI 1722 GCATTC GAATGC(1/-1)
BstAPI 358 GCACGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1759 GCTGAGC GC^T[ACGT]AGC
EcoNI 2599 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1271 GAATTC G^AATTC
HaeII 1021 GGCGCT [GA]GCGC^[CT]
HapII 2102 CCGG C^CGG
HpaII 2102 CCGG C^CGG
MfeI 3261 CAATTG C^AATTG
MspA1I 1459 CAGCTG C[AC]G^C[GT]G
MspI 2102 CCGG C^CGG
MunI 3261 CAATTG C^AATTG
PacI 3242 TTAATTAA TTAAT^TAA
PflFI 971 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1312 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 66 CTGCAG CTGCA^G
PvuII 1459 CAGCTG CAG^CTG
SacI 413 GAGCTC GAGCT^C
SexAI 2436 ACCAGGT A^CC[AT]GGT
StuI 288 AGGCCT AGG^CCT
Tth111I 971 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1312 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 533 CCAGTAACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1251 GTCTAC GT^[AC][GT]AC
3530 GTAGAC
AflII 2750 CTTAAG C^TTAAG
3013 CTTAAG
AseI 1510 ATTAAT AT^TAAT
3442 ATTAAT
AsnI 1510 ATTAAT AT^TAAT
3442 ATTAAT
AvrII 730 CCTAGG C^CTAGG
3756 CCTAGG
BalI 545 TGGCCA TGG^CCA
3591 TGGCCA
BclI 1674 TGATCA T^GATCA
2379 TGATCA
BfrI 2750 CTTAAG C^TTAAG
3013 CTTAAG
BlnI 730 CCTAGG C^CTAGG
3756 CCTAGG
BsaI 922 GAGACC GGTCTC(1/5)
2242 GAGACC
BseRI 1025 CTCCTC GAGGAG(10/8)
651 GAGGAG
CfoI 5 GCGC GCG^C
1022 GCGC
FbaI 1674 TGATCA T^GATCA
2379 TGATCA
HhaI 5 GCGC GCG^C
1022 GCGC
HinP1I 5 GCGC G^CGC
1022 GCGC
HindIII 1753 AAGCTT A^AGCTT
2347 AAGCTT
MluNI 545 TGGCCA TGG^CCA
3591 TGGCCA
MscI 545 TGGCCA TGG^CCA
3591 TGGCCA
NcoI 819 CCATGG C^CATGG
1053 CCATGG
PpuMI 1278 GGGACCT [GA]G^G[AT]CC[CT]
1362 AGGACCT
PshBI 1510 ATTAAT AT^TAAT
3442 ATTAAT
ScaI 439 AGTACT AGT^ACT
2982 AGTACT
SfcI 66 CTGCAG C^T[GA][CT]AG
637 CTACAG
TaqI 1161 TCGA T^CGA
2369 TCGA
TthHB8I 1161 TCGA T^CGA
2369 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BbsI BbvI BfaI BglII BmyI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HpaI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI