################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII BalI BbeI BbrPI BcgI BglI BlpI BpmI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HaeII Hin1I KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflII 1343 CTTAAG C^TTAAG AflIII 2087 ACATGT A^C[GA][CT]GT AocI 472 CCTCAGG CC^T[ACGT]AGG AseI 3818 ATTAAT AT^TAAT AsnI 3818 ATTAAT AT^TAAT AspHI 1395 GAGCTC G[AT]GC[AT]^C AvrII 2464 CCTAGG C^CTAGG BamHI 1762 GGATCC G^GATCC BanI 2604 GGTGCC G^G[CT][GA]CC BanII 1395 GAGCTC G[GA]GC[CT]^C BclI 1705 TGATCA T^GATCA BcnI 1065 CCCGG CC^[GC]GG BfrI 1343 CTTAAG C^TTAAG BlnI 2464 CCTAGG C^CTAGG BmyI 1395 GAGCTC G[AGT]GC[ACT]^C BsaAI 3873 TACGTG [CT]AC^GT[GA] BsgI 2893 CTGCAC GTGCAG(16/14) BsiHKAI 1395 GAGCTC G[AT]GC[AT]^C BsmI 415 GCATTC GAATGC(1/-1) Bsp1286I 1395 GAGCTC G[AGT]GC[ACT]^C BspLU11I 2087 ACATGT A^CATGT BsrDI 625 CATTGC GCAATG(2/0) BstBI 279 TTCGAA TT^CGAA Bsu36I 472 CCTCAGG CC^T[ACGT]AGG CfoI 3680 GCGC GCG^C DraII 1908 AGGCCCT [GA]G^G[ACGT]CC[CT] Eco81I 472 CCTCAGG CC^T[ACGT]AGG EcoNI 956 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1908 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 949 CCCGC CCCGC(4/6) FbaI 1705 TGATCA T^GATCA HapII 1066 CCGG C^CGG HgaI 1818 GCGTC GACGC(5/10) HhaI 3680 GCGC GCG^C HinP1I 3680 GCGC G^CGC HpaI 1481 GTTAAC GTT^AAC HpaII 1066 CCGG C^CGG MslI 1111 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1066 CCGG C^CGG NciI 1065 CCCGG CC^[GC]GG NdeI 2856 CATATG CA^TATG NspV 279 TTCGAA TT^CGAA PflMI 3392 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 3818 ATTAAT AT^TAAT SacI 1395 GAGCTC GAGCT^C SalI 153 GTCGAC G^TCGAC ScaI 2031 AGTACT AGT^ACT SfuI 279 TTCGAA TT^CGAA SpeI 3773 ACTAGT A^CTAGT Van91I 3392 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3709 TCTAGA T^CTAGA XcmI 1326 CCAGGATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 153 GTCGAC GT^[AC][GT]AC 2083 GTAGAC AlwNI 114 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 1446 CAGAACCTG BbsI 506 GTCTTC GAAGAC(2/6) 3402 GTCTTC BbvCI 1810 CCTCAGC CCTCAGC(-5/-2) 2576 GCTGAGG BpuAI 506 GTCTTC GAAGAC(2/6) 3402 GTCTTC BsaBI 431 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3647 GATTGAAATC BsaI 940 GAGACC GGTCTC(1/5) 1493 GAGACC BseRI 1808 CTCCTC GAGGAG(10/8) 174 GAGGAG BstXI 247 CCATGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 392 CCAGTCAACTGG EcoRI 788 GAATTC G^AATTC 870 GAATTC EcoRV 662 GATATC GAT^ATC 797 GATATC MamI 431 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3647 GATTGAAATC TaqI 154 TCGA T^CGA 280 TCGA TthHB8I 154 TCGA T^CGA 280 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbvI BciVI BfaI BglII BsaJI BsiYI BslI BsmAI BsmFI Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI