##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BbeI        
BbrPI       BcgI        BglI        BlpI        BpmI        
Bpu1102I    BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoT22I     
FseI        FspI        HaeII       Hin1I       KasI        
KpnI        KspI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SapI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SwaI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AflII        1343  CTTAAG               C^TTAAG
AflIII       2087  ACATGT               A^C[GA][CT]GT
AocI          472  CCTCAGG              CC^T[ACGT]AGG
AseI         3818  ATTAAT               AT^TAAT
AsnI         3818  ATTAAT               AT^TAAT
AspHI        1395  GAGCTC               G[AT]GC[AT]^C
AvrII        2464  CCTAGG               C^CTAGG
BamHI        1762  GGATCC               G^GATCC
BanI         2604  GGTGCC               G^G[CT][GA]CC
BanII        1395  GAGCTC               G[GA]GC[CT]^C
BclI         1705  TGATCA               T^GATCA
BcnI         1065  CCCGG                CC^[GC]GG
BfrI         1343  CTTAAG               C^TTAAG
BlnI         2464  CCTAGG               C^CTAGG
BmyI         1395  GAGCTC               G[AGT]GC[ACT]^C
BsaAI        3873  TACGTG               [CT]AC^GT[GA]
BsgI         2893  CTGCAC               GTGCAG(16/14)
BsiHKAI      1395  GAGCTC               G[AT]GC[AT]^C
BsmI          415  GCATTC               GAATGC(1/-1)
Bsp1286I     1395  GAGCTC               G[AGT]GC[ACT]^C
BspLU11I     2087  ACATGT               A^CATGT
BsrDI         625  CATTGC               GCAATG(2/0)
BstBI         279  TTCGAA               TT^CGAA
Bsu36I        472  CCTCAGG              CC^T[ACGT]AGG
CfoI         3680  GCGC                 GCG^C
DraII        1908  AGGCCCT              [GA]G^G[ACGT]CC[CT]
Eco81I        472  CCTCAGG              CC^T[ACGT]AGG
EcoNI         956  CCTGCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1908  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FauI          949  CCCGC                CCCGC(4/6)
FbaI         1705  TGATCA               T^GATCA
HapII        1066  CCGG                 C^CGG
HgaI         1818  GCGTC                GACGC(5/10)
HhaI         3680  GCGC                 GCG^C
HinP1I       3680  GCGC                 G^CGC
HpaI         1481  GTTAAC               GTT^AAC
HpaII        1066  CCGG                 C^CGG
MslI         1111  CATTGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1066  CCGG                 C^CGG
NciI         1065  CCCGG                CC^[GC]GG
NdeI         2856  CATATG               CA^TATG
NspV          279  TTCGAA               TT^CGAA
PflMI        3392  CCAAGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        3818  ATTAAT               AT^TAAT
SacI         1395  GAGCTC               GAGCT^C
SalI          153  GTCGAC               G^TCGAC
ScaI         2031  AGTACT               AGT^ACT
SfuI          279  TTCGAA               TT^CGAA
SpeI         3773  ACTAGT               A^CTAGT
Van91I       3392  CCAAGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3709  TCTAGA               T^CTAGA
XcmI         1326  CCAGGATGAGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI          153  GTCGAC               GT^[AC][GT]AC
             2083  GTAGAC              
AlwNI         114  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1446  CAGAACCTG           
BbsI          506  GTCTTC               GAAGAC(2/6)
             3402  GTCTTC              
BbvCI        1810  CCTCAGC              CCTCAGC(-5/-2)
             2576  GCTGAGG             
BpuAI         506  GTCTTC               GAAGAC(2/6)
             3402  GTCTTC              
BsaBI         431  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3647  GATTGAAATC          
BsaI          940  GAGACC               GGTCTC(1/5)
             1493  GAGACC              
BseRI        1808  CTCCTC               GAGGAG(10/8)
              174  GAGGAG              
BstXI         247  CCATGGGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              392  CCAGTCAACTGG        
EcoRI         788  GAATTC               G^AATTC
              870  GAATTC              
EcoRV         662  GATATC               GAT^ATC
              797  GATATC              
MamI          431  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3647  GATTGAAATC          
TaqI          154  TCGA                 T^CGA
              280  TCGA                
TthHB8I       154  TCGA                 T^CGA
              280  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BbvI        BciVI       
BfaI        BglII       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1407I    BspMI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DsaI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HincII      HindII      HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       XmnI