##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AhdI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       BbrPI       BfrI        BglII       BsaAI       
BsaBI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DrdI        Eam1105I    Eco81I      EcoRI       EcoRV       
FspI        HpaI        KpnI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        TfiI        
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         894  GACGTC               GACGT^C
AcsI          241  AAATTT               [GA]^AATT[CT]
AgeI         3854  ACCGGT               A^CCGGT
ApoI          241  AAATTT               [GA]^AATT[CT]
AvrII        3035  CCTAGG               C^CTAGG
BbsI         3834  GAAGAC               GAAGAC(2/6)
BcgI         3403  GCAATAAGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3578  GTATCC               GTATCC(6/5)
BclI         3924  TGATCA               T^GATCA
BlnI         3035  CCTAGG               C^CTAGG
BlpI          659  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      659  GCTGAGC              GC^T[ACGT]AGC
BpuAI        3834  GAAGAC               GAAGAC(2/6)
BspLU11I     1114  ACATGT               A^CATGT
BsrDI         746  CATTGC               GCAATG(2/0)
BssSI        3346  CTCGTG               CACGAG(-5/-1)
BstEII       1679  GGTGACC              G^GT[ACGT]ACC
BstPI        1679  GGTGACC              G^GT[ACGT]ACC
CelII         659  GCTGAGC              GC^T[ACGT]AGC
DraIII       3765  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1679  GGTGACC              G^GT[ACGT]ACC
FbaI         3924  TGATCA               T^GATCA
FseI           42  GGCCGGCC             GGCCGG^CC
HincII        390  GTTGAC               GT[CT]^[GA]AC
HindII        390  GTTGAC               GT[CT]^[GA]AC
HindIII      3561  AAGCTT               A^AGCTT
KspI           13  CCGCGG               CCGC^GG
PaeR7I         31  CTCGAG               C^TCGAG
PinAI        3854  ACCGGT               A^CCGGT
SacI         1443  GAGCTC               GAGCT^C
SacII          13  CCGCGG               CCGC^GG
SapI          173  GAAGAGC              GCTCTTC(1/4)
SbfI         3416  CCTGCAGG             CCTGCA^GG
SexAI        3212  ACCTGGT              A^CC[AT]GGT
SfiI         3728  GGCCCTCTAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI           31  CTCGAG               C^T[CT][GA]AG
Sse8387I     3416  CCTGCAGG             CCTGCA^GG
XhoI           31  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII       1114  ACATGT               A^C[GA][CT]GT
             2677  ACGTGT              
Aor51HI       833  AGCGCT               AGC^GCT
             1073  AGCGCT              
Asp700I      1289  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2642  GAAAGCCTTC          
AspHI        1443  GAGCTC               G[AT]GC[AT]^C
             3396  GAGCAC              
BbeI         1389  GGCGCC               GGCGC^C
             2403  GGCGCC              
BsiHKAI      1443  GAGCTC               G[AT]GC[AT]^C
             3396  GAGCAC              
BstAPI       2763  GCAGCGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3764  GCACAGGGTGC         
Eco47III      833  AGCGCT               AGC^GCT
             1073  AGCGCT              
EcoNI        2334  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3353  CCTGCTGGAGG         
EcoT22I      1532  ATGCAT               ATGCA^T
             3641  ATGCAT              
KasI         1389  GGCGCC               G^GCGCC
             2403  GGCGCC              
NarI         1389  GGCGCC               GG^CGCC
             2403  GGCGCC              
NcoI         2773  CCATGG               C^CATGG
             3070  CCATGG              
NsiI         1532  ATGCAT               ATGCA^T
             3641  ATGCAT              
NspI          413  GCATGT               [GA]CATG^[CT]
             1114  ACATGT              
PflMI        2280  CCAGAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2482  CCAGTGTGTGG         
SfoI         1389  GGCGCC               GGC^GCC
             2403  GGCGCC              
Van91I       2280  CCAGAGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2482  CCAGTGTGTGG         
XmnI         1289  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2642  GAAAGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AvaI        AvaII       
BalI        BamHI       BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmaCI       XmaI