##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspI        BbeI        BbsI        
BclI        BfrI        BglI        BpuAI       BsaWI       
BseAI       BsiWI       BsmI        BspDI       BspEI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        EagI        
EclXI       Eco47III    Eco52I      EcoRV       FbaI        
FseI        HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmeI        PshBI       
Psp1406I    RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          416  GTCTAC               GT^[AC][GT]AC
AhdI         2117  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      3867  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2117  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3177  TGCGCA               TGC^GCA
AvrII        4274  CCTAGG               C^CTAGG
BamHI        2725  GGATCC               G^GATCC
BciVI        1374  GTATCC               GTATCC(6/5)
BlnI         4274  CCTAGG               C^CTAGG
BlpI         3013  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3013  GCTCAGC              GC^T[ACGT]AGC
BsaBI         364  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1054  CGATCG               CG[GA][CT]^CG
Bsp1407I     4111  TGTACA               T^GTACA
BspHI        2037  TCATGA               T^CATGA
BsrFI        3212  GCCGGC               [GA]^CCGG[CT]
BsrGI        4111  TGTACA               T^GTACA
BstEII       3041  GGTCACC              G^GT[ACGT]ACC
BstPI        3041  GGTCACC              G^GT[ACGT]ACC
CelII        3013  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       3212  GCCGGC               [GA]^CCGG[CT]
DrdI          695  GACAACACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2117  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3041  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2214  ATGCAT               ATGCA^T
FspI         3177  TGCGCA               TGC^GCA
HindIII      3868  AAGCTT               A^AGCTT
MamI          364  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          232  TTAA                 T^TAA
NaeI         3212  GCCGGC               GCC^GGC
NgoMIV       3212  GCCGGC               G^CCGGC
NsiI         2214  ATGCAT               ATGCA^T
PaeR7I       1934  CTCGAG               C^TCGAG
PflMI        1343  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        3902  GACCATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         1054  CGATCG               CGAT^CG
RcaI         2037  TCATGA               T^CATGA
SacI         2783  GAGCTC               GAGCT^C
SmaI         4105  CCCGGG               CCC^GGG
SspBI        4111  TGTACA               T^GTACA
Tru9I         232  TTAA                 T^TAA
Van91I       1343  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          201  TCTAGA               T^CTAGA
XhoI         1934  CTCGAG               C^TCGAG
XmaCI        4105  CCCGGG               C^CCGGG
XmaI         4105  CCCGGG               C^CCGGG
XmnI         3867  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII        2192  CGCG                 CG^CG
             3200  CGCG                
Alw44I       1574  GTGCAC               G^TGCAC
             2678  GTGCAC              
AocI         2851  CCTGAGG              CC^T[ACGT]AGG
             4027  CCTGAGG             
ApaLI        1574  GTGCAC               G^TGCAC
             2678  GTGCAC              
BalI         1446  TGGCCA               TGG^CCA
             4069  TGGCCA              
BbvCI        3893  CCTCAGC              CCTCAGC(-5/-2)
             1268  GCTGAGG             
BcgI         1562  GCAGGACTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1968  GCATCTCCTTCG        
BsrBI        1478  GAGCGG               CCGCTC(-3/-3)
             2732  GAGCGG              
BsrDI        2946  GCAATG               GCAATG(2/0)
             3781  GCAATG              
BstUI        2192  CGCG                 CG^CG
             3200  CGCG                
Bsu36I       2851  CCTGAGG              CC^T[ACGT]AGG
             4027  CCTGAGG             
DraIII       1341  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2606  CACCAGGTG           
Eco81I       2851  CCTGAGG              CC^T[ACGT]AGG
             4027  CCTGAGG             
EcoRI        2951  GAATTC               G^AATTC
             3474  GAATTC              
HaeII        1483  GGCGCT               [GA]GCGC^[CT]
             3110  AGCGCC              
HincII        565  GTCAAC               GT[CT]^[GA]AC
              977  GTCAAC              
HindII        565  GTCAAC               GT[CT]^[GA]AC
              977  GTCAAC              
MluNI        1446  TGGCCA               TGG^CCA
             4069  TGGCCA              
MscI         1446  TGGCCA               TGG^CCA
             4069  TGGCCA              
MvnI         2192  CGCG                 CG^CG
             3200  CGCG                
StuI          903  AGGCCT               AGG^CCT
             3445  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbrPI       
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII