################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI Asp718I AspI BbeI BbsI BclI BfrI BglI BpuAI BsaWI BseAI BsiWI BsmI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoRV FbaI FseI HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 416 GTCTAC GT^[AC][GT]AC AhdI 2117 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 3867 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2117 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3177 TGCGCA TGC^GCA AvrII 4274 CCTAGG C^CTAGG BamHI 2725 GGATCC G^GATCC BciVI 1374 GTATCC GTATCC(6/5) BlnI 4274 CCTAGG C^CTAGG BlpI 3013 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3013 GCTCAGC GC^T[ACGT]AGC BsaBI 364 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1054 CGATCG CG[GA][CT]^CG Bsp1407I 4111 TGTACA T^GTACA BspHI 2037 TCATGA T^CATGA BsrFI 3212 GCCGGC [GA]^CCGG[CT] BsrGI 4111 TGTACA T^GTACA BstEII 3041 GGTCACC G^GT[ACGT]ACC BstPI 3041 GGTCACC G^GT[ACGT]ACC CelII 3013 GCTCAGC GC^T[ACGT]AGC Cfr10I 3212 GCCGGC [GA]^CCGG[CT] DrdI 695 GACAACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2117 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3041 GGTCACC G^GT[ACGT]ACC EcoT22I 2214 ATGCAT ATGCA^T FspI 3177 TGCGCA TGC^GCA HindIII 3868 AAGCTT A^AGCTT MamI 364 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 232 TTAA T^TAA NaeI 3212 GCCGGC GCC^GGC NgoMIV 3212 GCCGGC G^CCGGC NsiI 2214 ATGCAT ATGCA^T PaeR7I 1934 CTCGAG C^TCGAG PflMI 1343 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3902 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1054 CGATCG CGAT^CG RcaI 2037 TCATGA T^CATGA SacI 2783 GAGCTC GAGCT^C SmaI 4105 CCCGGG CCC^GGG SspBI 4111 TGTACA T^GTACA Tru9I 232 TTAA T^TAA Van91I 1343 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 201 TCTAGA T^CTAGA XhoI 1934 CTCGAG C^TCGAG XmaCI 4105 CCCGGG C^CCGGG XmaI 4105 CCCGGG C^CCGGG XmnI 3867 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 2192 CGCG CG^CG 3200 CGCG Alw44I 1574 GTGCAC G^TGCAC 2678 GTGCAC AocI 2851 CCTGAGG CC^T[ACGT]AGG 4027 CCTGAGG ApaLI 1574 GTGCAC G^TGCAC 2678 GTGCAC BalI 1446 TGGCCA TGG^CCA 4069 TGGCCA BbvCI 3893 CCTCAGC CCTCAGC(-5/-2) 1268 GCTGAGG BcgI 1562 GCAGGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1968 GCATCTCCTTCG BsrBI 1478 GAGCGG CCGCTC(-3/-3) 2732 GAGCGG BsrDI 2946 GCAATG GCAATG(2/0) 3781 GCAATG BstUI 2192 CGCG CG^CG 3200 CGCG Bsu36I 2851 CCTGAGG CC^T[ACGT]AGG 4027 CCTGAGG DraIII 1341 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2606 CACCAGGTG Eco81I 2851 CCTGAGG CC^T[ACGT]AGG 4027 CCTGAGG EcoRI 2951 GAATTC G^AATTC 3474 GAATTC HaeII 1483 GGCGCT [GA]GCGC^[CT] 3110 AGCGCC HincII 565 GTCAAC GT[CT]^[GA]AC 977 GTCAAC HindII 565 GTCAAC GT[CT]^[GA]AC 977 GTCAAC MluNI 1446 TGGCCA TGG^CCA 4069 TGGCCA MscI 1446 TGGCCA TGG^CCA 4069 TGGCCA MvnI 2192 CGCG CG^CG 3200 CGCG StuI 903 AGGCCT AGG^CCT 3445 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbrPI BbvI BcnI BfaI BglII BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII