##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BbrPI       BcgI        BclI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsiWI       
Bsp1407I    BspDI       BspLU11I    BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Cfr10I      ClaI        CpoI        DraIII      
DrdI        Eam1105I    EcoO65I     EcoRV       FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        MamI        MfeI        MluI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacI        SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccIII        805  TCCGGA               T^CCGGA
Asp700I      3743  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1594  GGATCC               G^GATCC
BciVI        2492  GGATAC               GTATCC(6/5)
BlpI         2130  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2130  GCTCAGC              GC^T[ACGT]AGC
BseAI         805  TCCGGA               T^CCGGA
BsiEI        2810  CGGCCG               CG[GA][CT]^CG
BsmI         1094  GCATTC               GAATGC(1/-1)
BspEI         805  TCCGGA               T^CCGGA
BspHI        1480  TCATGA               T^CATGA
CelII        2130  GCTCAGC              GC^T[ACGT]AGC
DraI         4136  TTTAAA               TTT^AAA
EagI         2810  CGGCCG               C^GGCCG
EclXI        2810  CGGCCG               C^GGCCG
Eco52I       2810  CGGCCG               C^GGCCG
EcoNI         733  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        4144  GAATTC               G^AATTC
KspI         2517  CCGCGG               CCGC^GG
MaeII         984  ACGT                 A^CGT
MroI          805  TCCGGA               T^CCGGA
NdeI            3  CATATG               CA^TATG
NspI         1503  ACATGC               [GA]CATG^[CT]
PshAI        3265  GACTAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1480  TCATGA               T^CATGA
SacII        2517  CCGCGG               CCGC^GG
ScaI         2070  AGTACT               AGT^ACT
SspI         1946  AATATT               AAT^ATT
XmnI         3743  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII        2147  CGCG                 CG^CG
             2518  CGCG                
AcyI          742  GGCGCC               G[GA]^CG[CT]C
             2889  GGCGCC              
Aor51HI      1032  AGCGCT               AGC^GCT
             1116  AGCGCT              
AviII        1266  TGCGCA               TGC^GCA
             3057  TGCGCA              
BbeI          742  GGCGCC               GGCGC^C
             2889  GGCGCC              
BbsI          131  GAAGAC               GAAGAC(2/6)
             1496  GTCTTC              
BglI          545  GCCCGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2121  GCCCAACTGGC         
BpuAI         131  GAAGAC               GAAGAC(2/6)
             1496  GTCTTC              
BsaHI         742  GGCGCC               G[GA]^CG[CT]C
             2889  GGCGCC              
BsaI         3030  GAGACC               GGTCTC(1/5)
             3898  GGTCTC              
BsmBI         668  GAGACG               CGTCTC(1/5)
             3868  GAGACG              
BsrBI         488  CCGCTC               CCGCTC(-3/-3)
             2098  CCGCTC              
BsrDI        1692  CATTGC               GCAATG(2/0)
             3577  GCAATG              
BssSI           9  CACGAG               CACGAG(-5/-1)
             2041  CACGAG              
BstAPI       1875  GCACACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2424  GCATGGTGTGC         
BstUI        2147  CGCG                 CG^CG
             2518  CGCG                
Eco47III     1032  AGCGCT               AGC^GCT
             1116  AGCGCT              
EcoT22I      1528  ATGCAT               ATGCA^T
             2368  ATGCAT              
FspI         1266  TGCGCA               TGC^GCA
             3057  TGCGCA              
HgaI          670  GACGC                GACGC(5/10)
              609  GCGTC               
Hin1I         742  GGCGCC               G[GA]^CG[CT]C
             2889  GGCGCC              
KasI          742  GGCGCC               G^GCGCC
             2889  GGCGCC              
MvnI         2147  CGCG                 CG^CG
             2518  CGCG                
NarI          742  GGCGCC               GG^CGCC
             2889  GGCGCC              
NsiI         1528  ATGCAT               ATGCA^T
             2368  ATGCAT              
SapI         1023  GAAGAGC              GCTCTTC(1/4)
             2859  GAAGAGC             
SfoI          742  GGCGCC               GGC^GCC
             2889  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbvCI       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflFI       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XhoII       XmaCI       XmaI