################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AvrII BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraIII DrdI Eam1105I EcoO65I EcoRV FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccIII 805 TCCGGA T^CCGGA Asp700I 3743 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 1594 GGATCC G^GATCC BciVI 2492 GGATAC GTATCC(6/5) BlpI 2130 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2130 GCTCAGC GC^T[ACGT]AGC BseAI 805 TCCGGA T^CCGGA BsiEI 2810 CGGCCG CG[GA][CT]^CG BsmI 1094 GCATTC GAATGC(1/-1) BspEI 805 TCCGGA T^CCGGA BspHI 1480 TCATGA T^CATGA CelII 2130 GCTCAGC GC^T[ACGT]AGC DraI 4136 TTTAAA TTT^AAA EagI 2810 CGGCCG C^GGCCG EclXI 2810 CGGCCG C^GGCCG Eco52I 2810 CGGCCG C^GGCCG EcoNI 733 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4144 GAATTC G^AATTC KspI 2517 CCGCGG CCGC^GG MaeII 984 ACGT A^CGT MroI 805 TCCGGA T^CCGGA NdeI 3 CATATG CA^TATG NspI 1503 ACATGC [GA]CATG^[CT] PshAI 3265 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1480 TCATGA T^CATGA SacII 2517 CCGCGG CCGC^GG ScaI 2070 AGTACT AGT^ACT SspI 1946 AATATT AAT^ATT XmnI 3743 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 2147 CGCG CG^CG 2518 CGCG AcyI 742 GGCGCC G[GA]^CG[CT]C 2889 GGCGCC Aor51HI 1032 AGCGCT AGC^GCT 1116 AGCGCT AviII 1266 TGCGCA TGC^GCA 3057 TGCGCA BbeI 742 GGCGCC GGCGC^C 2889 GGCGCC BbsI 131 GAAGAC GAAGAC(2/6) 1496 GTCTTC BglI 545 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2121 GCCCAACTGGC BpuAI 131 GAAGAC GAAGAC(2/6) 1496 GTCTTC BsaHI 742 GGCGCC G[GA]^CG[CT]C 2889 GGCGCC BsaI 3030 GAGACC GGTCTC(1/5) 3898 GGTCTC BsmBI 668 GAGACG CGTCTC(1/5) 3868 GAGACG BsrBI 488 CCGCTC CCGCTC(-3/-3) 2098 CCGCTC BsrDI 1692 CATTGC GCAATG(2/0) 3577 GCAATG BssSI 9 CACGAG CACGAG(-5/-1) 2041 CACGAG BstAPI 1875 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2424 GCATGGTGTGC BstUI 2147 CGCG CG^CG 2518 CGCG Eco47III 1032 AGCGCT AGC^GCT 1116 AGCGCT EcoT22I 1528 ATGCAT ATGCA^T 2368 ATGCAT FspI 1266 TGCGCA TGC^GCA 3057 TGCGCA HgaI 670 GACGC GACGC(5/10) 609 GCGTC Hin1I 742 GGCGCC G[GA]^CG[CT]C 2889 GGCGCC KasI 742 GGCGCC G^GCGCC 2889 GGCGCC MvnI 2147 CGCG CG^CG 2518 CGCG NarI 742 GGCGCC GG^CGCC 2889 GGCGCC NsiI 1528 ATGCAT ATGCA^T 2368 ATGCAT SapI 1023 GAAGAGC GCTCTTC(1/4) 2859 GAAGAGC SfoI 742 GGCGCC GGC^GCC 2889 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmaCI XmaI