##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BciVI BclI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiWI Bsp1407I BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstZ17I
CelII CpoI Eam1105I Eco47III EcoT22I
FbaI FseI FspI HpaI KpnI
MamI MluI MroI NdeI NheI
NotI NruI NsiI PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SgrAI SnaBI Sse8387I
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3277 GACGTC GACGT^C
AflII 427 CTTAAG C^TTAAG
AflIII 5002 ACGTGT A^C[GA][CT]GT
Alw44I 2940 GTGCAC G^TGCAC
ApaLI 2940 GTGCAC G^TGCAC
AspI 64 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 7 GGCGCC GGCGC^C
BcgI 1391 GCATGAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 427 CTTAAG C^TTAAG
BglI 3678 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 962 ACCGGA [AT]^CCGG[AT]
BsiEI 3900 CGGCCG CG[GA][CT]^CG
BsmI 2384 GCATTC GAATGC(1/-1)
BspDI 2612 ATCGAT AT^CGAT
ClaI 2612 ATCGAT AT^CGAT
DrdI 2788 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3900 CGGCCG C^GGCCG
EclXI 3900 CGGCCG C^GGCCG
Eco52I 3900 CGGCCG C^GGCCG
HincII 1336 GTTGAC GT[CT]^[GA]AC
HindII 1336 GTTGAC GT[CT]^[GA]AC
KasI 7 GGCGCC G^GCGCC
KspI 3038 CCGCGG CCGC^GG
MfeI 197 CAATTG C^AATTG
MslI 4748 CATGCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 197 CAATTG C^AATTG
NaeI 1829 GCCGGC GCC^GGC
NarI 7 GGCGCC GG^CGCC
NgoMIV 1829 GCCGGC G^CCGGC
PaeR7I 578 CTCGAG C^TCGAG
PflFI 64 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3367 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 713 GAGCTC GAGCT^C
SacII 3038 CCGCGG CCGC^GG
SfoI 7 GGCGCC GGC^GCC
SpeI 4672 ACTAGT A^CTAGT
Tth111I 64 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 578 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 3135 GGGCCC GGGCC^C
4873 GGGCCC
BamHI 1292 GGATCC G^GATCC
2673 GGATCC
BbrPI 2287 CACGTG CAC^GTG
5001 CACGTG
BsrDI 1368 CATTGC GCAATG(2/0)
1220 GCAATG
BsrFI 1829 GCCGGC [GA]^CCGG[CT]
2204 ACCGGC
BssSI 2233 CACGAG CACGAG(-5/-1)
1342 CTCGTG
BstBI 1520 TTCGAA TT^CGAA
2054 TTCGAA
BstEII 532 GGTGACC G^GT[ACGT]ACC
1680 GGTGACC
BstPI 532 GGTGACC G^GT[ACGT]ACC
1680 GGTGACC
Cfr10I 1829 GCCGGC [GA]^CCGG[CT]
2204 ACCGGC
DraI 849 TTTAAA TTT^AAA
4763 TTTAAA
EcoO65I 532 GGTGACC G^GT[ACGT]ACC
1680 GGTGACC
NspV 1520 TTCGAA TT^CGAA
2054 TTCGAA
PflMI 2232 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4852 CCAGCCTTTGG
PmaCI 2287 CACGTG CAC^GTG
5001 CACGTG
PmlI 2287 CACGTG CAC^GTG
5001 CACGTG
SfuI 1520 TTCGAA TT^CGAA
2054 TTCGAA
SmaI 3022 CCCGGG CCC^GGG
5008 CCCGGG
SphI 2156 GCATGC GCATG^C
3732 GCATGC
StuI 3044 AGGCCT AGG^CCT
4199 AGGCCT
Van91I 2232 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4852 CCAGCCTTTGG
XmaCI 3022 CCCGGG C^CCGGG
5008 CCCGGG
XmaI 3022 CCCGGG C^CCGGG
5008 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI