##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        AscI        
AseI        AsnI        AspEI       AvrII       BbeI        
BcgI        BciVI       BlnI        BlpI        Bpu1102I    
BsaBI       BsiWI       Bsp1407I    BspDI       BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     CelII       ClaI        CpoI        Eam1105I    
EcoRV       EcoT22I     HpaI        KasI        KspI        
MamI        MfeI        MluI        MunI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SbfI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        329  GGTACC               G^GTACC
AccI         3636  GTCGAC               GT^[AC][GT]AC
AcyI          280  GACGCC               G[GA]^CG[CT]C
AflII         923  CTTAAG               C^TTAAG
AflIII       2692  ACATGT               A^C[GA][CT]GT
Alw44I       2344  GTGCAC               G^TGCAC
ApaLI        2344  GTGCAC               G^TGCAC
Asp700I      2767  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       329  GGTACC               G^GTACC
AviII        1746  TGCGCA               TGC^GCA
BbrPI        3966  CACGTG               CAC^GTG
BfrI          923  CTTAAG               C^TTAAG
BsaAI        3966  CACGTG               [CT]AC^GT[GA]
BsaHI         280  GACGCC               G[GA]^CG[CT]C
BsaI         1473  GAGACC               GGTCTC(1/5)
BspHI         750  TCATGA               T^CATGA
BspLU11I     2692  ACATGT               A^CATGT
BssSI        2393  CTCGTG               CACGAG(-5/-1)
BstAPI       1396  GCAGGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI         2543  TTTAAA               TTT^AAA
EcoRI         554  GAATTC               G^AATTC
FseI         3488  GGCCGGCC             GGCCGG^CC
FspI         1746  TGCGCA               TGC^GCA
Hin1I         280  GACGCC               G[GA]^CG[CT]C
KpnI          329  GGTACC               GGTAC^C
NheI          913  GCTAGC               G^CTAGC
PmaCI        3966  CACGTG               CAC^GTG
PmlI         3966  CACGTG               CAC^GTG
PshAI        2494  GACCATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          750  TCATGA               T^CATGA
SalI         3636  GTCGAC               G^TCGAC
ScaI          489  AGTACT               AGT^ACT
XcmI          811  CCACGCCAGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2767  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AccII        1491  CGCG                 CG^CG
             2488  CGCG                
AccIII        507  TCCGGA               T^CCGGA
             2856  TCCGGA              
AcsI          554  GAATTC               [GA]^AATT[CT]
             1196  AAATTC              
Aor51HI        99  AGCGCT               AGC^GCT
              384  AGCGCT              
ApoI          554  GAATTC               [GA]^AATT[CT]
             1196  AAATTC              
AspI         2015  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2621  GACGTGGTC           
BamHI        3115  GGATCC               G^GATCC
             3496  GGATCC              
BbsI         2206  GAAGAC               GAAGAC(2/6)
             1238  GTCTTC              
BbvCI        2864  GCTGAGG              CCTCAGC(-5/-2)
             3353  GCTGAGG             
BclI         3213  TGATCA               T^GATCA
             3306  TGATCA              
BglII         420  AGATCT               A^GATCT
             1590  AGATCT              
BpuAI        2206  GAAGAC               GAAGAC(2/6)
             1238  GTCTTC              
BsaWI         507  TCCGGA               [AT]^CCGG[AT]
             2856  TCCGGA              
BseAI         507  TCCGGA               T^CCGGA
             2856  TCCGGA              
BsiEI         182  CGGCCG               CG[GA][CT]^CG
             3491  CGGCCG              
BsmBI         278  GAGACG               CGTCTC(1/5)
              476  GAGACG              
BsmI         1085  GAATGC               GAATGC(1/-1)
             3405  GCATTC              
BspEI         507  TCCGGA               T^CCGGA
             2856  TCCGGA              
BstUI        1491  CGCG                 CG^CG
             2488  CGCG                
DrdI          200  GACCTGACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1718  GACCTGAGGGTC        
EagI          182  CGGCCG               C^GGCCG
             3491  CGGCCG              
EclXI         182  CGGCCG               C^GGCCG
             3491  CGGCCG              
Eco47III       99  AGCGCT               AGC^GCT
              384  AGCGCT              
Eco52I        182  CGGCCG               C^GGCCG
             3491  CGGCCG              
FbaI         3213  TGATCA               T^GATCA
             3306  TGATCA              
HindIII      2723  AAGCTT               A^AGCTT
             2759  AAGCTT              
MroI          507  TCCGGA               T^CCGGA
             2856  TCCGGA              
MvnI         1491  CGCG                 CG^CG
             2488  CGCG                
PflFI        2015  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2621  GACGTGGTC           
PflMI         763  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1348  CCAGGCAGTGG         
SacI          845  GAGCTC               GAGCT^C
             1213  GAGCTC              
SapI          721  GAAGAGC              GCTCTTC(1/4)
             1540  GAAGAGC             
SexAI         951  ACCTGGT              A^CC[AT]GGT
             2016  ACCTGGT             
SmaI         2165  CCCGGG               CCC^GGG
             2871  CCCGGG              
Tth111I      2015  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2621  GACGTGGTC           
Van91I        763  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1348  CCAGGCAGTGG         
XmaCI        2165  CCCGGG               C^CCGGG
             2871  CCCGGG              
XmaI         2165  CCCGGG               C^CCGGG
             2871  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII