##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AccI        AccIII      AgeI        AscI        AseI        
AsnI        AviII       AvrII       BbrPI       BcgI        
BciVI       BclI        BglI        BglII       BlnI        
BsaAI       BsaBI       BseAI       BseRI       BsiWI       
BsmI        BspDI       BspEI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        EagI        EclXI       
Eco52I      EcoO65I     EcoT22I     FbaI        FseI        
FspI        HpaI        KspI        MamI        MfeI        
MroI        MunI        NaeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII          72  GACGTC               GACGT^C
Acc65I       3020  GGTACC               G^GTACC
AclI          324  AACGTT               AA^CGTT
AflII        2549  CTTAAG               C^TTAAG
AhdI         2864  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       344  AGCGCT               AGC^GCT
Asp718I      3020  GGTACC               G^GTACC
AspEI        2864  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2831  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2318  TGGCCA               TGG^CCA
BamHI         932  GGATCC               G^GATCC
BbeI          899  GGCGCC               GGCGC^C
BfrI         2549  CTTAAG               C^TTAAG
BlpI         4072  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     4072  GCTAAGC              GC^T[ACGT]AGC
BsaWI        2724  ACCGGA               [AT]^CCGG[AT]
BsiEI        1373  CGGTCG               CG[GA][CT]^CG
Bsp1407I     3792  TGTACA               T^GTACA
BspLU11I     3659  ACATGT               A^CATGT
BspMI         983  GCAGGT               ACCTGC(4/8)
BsrBI          50  GAGCGG               CCGCTC(-3/-3)
BsrFI        3262  GCCGGT               [GA]^CCGG[CT]
BsrGI        3792  TGTACA               T^GTACA
BstXI        1994  CCACCTGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        4072  GCTAAGC              GC^T[ACGT]AGC
Cfr10I       3262  GCCGGT               [GA]^CCGG[CT]
DraI         2505  TTTAAA               TTT^AAA
DraIII        891  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           72  GACGTCCGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2864  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      344  AGCGCT               AGC^GCT
EcoRV        2214  GATATC               GAT^ATC
KasI          899  GGCGCC               G^GCGCC
KpnI         3020  GGTACC               GGTAC^C
MluI         1820  ACGCGT               A^CGCGT
MluNI        2318  TGGCCA               TGG^CCA
MscI         2318  TGGCCA               TGG^CCA
NarI          899  GGCGCC               GG^CGCC
NcoI         2822  CCATGG               C^CATGG
NspI         3659  ACATGT               [GA]CATG^[CT]
PflFI        2831  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      324  AACGTT               AA^CGTT
SacI          852  GAGCTC               GAGCT^C
SapI         1196  GAAGAGC              GCTCTTC(1/4)
SfoI          899  GGCGCC               GGC^GCC
SmaI          383  CCCGGG               CCC^GGG
SspBI        3792  TGTACA               T^GTACA
Tth111I      2831  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          838  CCAGGGACAATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         383  CCCGGG               C^CCGGG
XmaI          383  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI           72  GACGTC               G[GA]^CG[CT]C
              899  GGCGCC              
AflIII       1820  ACGCGT               A^C[GA][CT]GT
             3659  ACATGT              
ApaI           62  GGGCCC               GGGCC^C
              106  GGGCCC              
BbvCI        3024  CCTCAGC              CCTCAGC(-5/-2)
              426  GCTGAGG             
BsaHI          72  GACGTC               G[GA]^CG[CT]C
              899  GGCGCC              
BsmBI         727  CGTCTC               CGTCTC(1/5)
               70  GAGACG              
BspHI        2381  TCATGA               T^CATGA
             2911  TCATGA              
DsaI          689  CCGTGG               C^C[GA][CT]GG
             2822  CCATGG              
EaeI          557  TGGCCG               [CT]^GGCC[GA]
             2318  TGGCCA              
EcoNI         515  CCTGGGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3934  CCTCACCCAGG         
Hin1I          72  GACGTC               G[GA]^CG[CT]C
              899  GGCGCC              
HindIII      1724  AAGCTT               A^AGCTT
             2930  AAGCTT              
NdeI         1663  CATATG               CA^TATG
             3783  CATATG              
PflMI        3891  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3965  CCAACACCTGG         
PstI          669  CTGCAG               CTGCA^G
             2856  CTGCAG              
RcaI         2381  TCATGA               T^CATGA
             2911  TCATGA              
SexAI        1303  ACCTGGT              A^CC[AT]GGT
             3970  ACCTGGT             
SspI         1489  AATATT               AAT^ATT
             3770  AATATT              
Van91I       3891  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3965  CCAACACCTGG         
XbaI         2804  TCTAGA               T^CTAGA
             3042  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        Asp700I     AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        Eco81I      
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI