##################################
# NO CUT ENZYMES (   26 enzymes) #
##################################

AatII       Acc65I      AscI        AseI        AsnI        
Asp718I     BbrPI       CpoI        DraI        FseI        
HpaI        KpnI        KspI        NotI        PacI        
PmaCI       PmeI        PmlI        PshBI       PvuI        
RsrII       SacII       SgrAI       SnaBI       SpeI        
SwaI        


###################################
# ONE CUT ENZYMES (   19 enzymes) #
###################################

AflII       13566  CTTAAG               C^TTAAG
AgeI        13687  ACCGGT               A^CCGGT
BfrI        13566  CTTAAG               C^TTAAG
BsiWI        9000  CGTACG               C^GTACG
BspDI       11520  ATCGAT               AT^CGAT
BssHII       3102  GCGCGC               G^CGCGC
ClaI        11520  ATCGAT               AT^CGAT
NdeI        12525  CATATG               CA^TATG
NheI        13991  GCTAGC               G^CTAGC
PaeR7I      10239  CTCGAG               C^TCGAG
PinAI       13687  ACCGGT               A^CCGGT
PshAI        1276  GACTTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI         8637  GTCGAC               G^TCGAC
SbfI         6870  CCTGCAGG             CCTGCA^GG
ScaI         6763  AGTACT               AGT^ACT
Sse8387I     6870  CCTGCAGG             CCTGCA^GG
SspI          600  AATATT               AAT^ATT
XbaI        10721  TCTAGA               T^CTAGA
XhoI        10239  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AclI          984  AACGTT               AA^CGTT
            14361  AACGTT              
Alw44I       3111  GTGCAC               G^TGCAC
             3201  GTGCAC              
Aor51HI      9262  AGCGCT               AGC^GCT
            13045  AGCGCT              
ApaLI        3111  GTGCAC               G^TGCAC
             3201  GTGCAC              
AviII        3463  TGCGCA               TGC^GCA
            11863  TGCGCA              
BsaBI       12824  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
            13209  GATCCTCATC          
BsmBI       11024  CGTCTC               CGTCTC(1/5)
             3417  GAGACG              
EagI           26  CGGCCG               C^GGCCG
            11018  CGGCCG              
EclXI          26  CGGCCG               C^GGCCG
            11018  CGGCCG              
Eco47III     9262  AGCGCT               AGC^GCT
            13045  AGCGCT              
Eco52I         26  CGGCCG               C^GGCCG
            11018  CGGCCG              
EcoRI        4101  GAATTC               G^AATTC
             4962  GAATTC              
EcoRV        9858  GATATC               GAT^ATC
            11010  GATATC              
EcoT22I      2312  ATGCAT               ATGCA^T
             9288  ATGCAT              
FspI         3463  TGCGCA               TGC^GCA
            11863  TGCGCA              
MamI        12824  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
            13209  GATCCTCATC          
MluI          385  ACGCGT               A^CGCGT
            10100  ACGCGT              
NsiI         2312  ATGCAT               ATGCA^T
             9288  ATGCAT              
Psp1406I      984  AACGTT               AA^CGTT
            14361  AACGTT              


#########################################
# 3 OR MORE CUT ENZYMES (  194 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AflIII      AhdI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AvaII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        Eam1105I    EarI        Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MfeI        
MflI        MluNI       MnlI        MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NruI        NspI        NspV        PflFI       
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SmaI        SmlI        
SphI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        XmnI