################################## # NO CUT ENZYMES ( 26 enzymes) # ################################## AatII Acc65I AscI AseI AsnI Asp718I BbrPI CpoI DraI FseI HpaI KpnI KspI NotI PacI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 19 enzymes) # ################################### AflII 13566 CTTAAG C^TTAAG AgeI 13687 ACCGGT A^CCGGT BfrI 13566 CTTAAG C^TTAAG BsiWI 9000 CGTACG C^GTACG BspDI 11520 ATCGAT AT^CGAT BssHII 3102 GCGCGC G^CGCGC ClaI 11520 ATCGAT AT^CGAT NdeI 12525 CATATG CA^TATG NheI 13991 GCTAGC G^CTAGC PaeR7I 10239 CTCGAG C^TCGAG PinAI 13687 ACCGGT A^CCGGT PshAI 1276 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 8637 GTCGAC G^TCGAC SbfI 6870 CCTGCAGG CCTGCA^GG ScaI 6763 AGTACT AGT^ACT Sse8387I 6870 CCTGCAGG CCTGCA^GG SspI 600 AATATT AAT^ATT XbaI 10721 TCTAGA T^CTAGA XhoI 10239 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AclI 984 AACGTT AA^CGTT 14361 AACGTT Alw44I 3111 GTGCAC G^TGCAC 3201 GTGCAC Aor51HI 9262 AGCGCT AGC^GCT 13045 AGCGCT ApaLI 3111 GTGCAC G^TGCAC 3201 GTGCAC AviII 3463 TGCGCA TGC^GCA 11863 TGCGCA BsaBI 12824 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 13209 GATCCTCATC BsmBI 11024 CGTCTC CGTCTC(1/5) 3417 GAGACG EagI 26 CGGCCG C^GGCCG 11018 CGGCCG EclXI 26 CGGCCG C^GGCCG 11018 CGGCCG Eco47III 9262 AGCGCT AGC^GCT 13045 AGCGCT Eco52I 26 CGGCCG C^GGCCG 11018 CGGCCG EcoRI 4101 GAATTC G^AATTC 4962 GAATTC EcoRV 9858 GATATC GAT^ATC 11010 GATATC EcoT22I 2312 ATGCAT ATGCA^T 9288 ATGCAT FspI 3463 TGCGCA TGC^GCA 11863 TGCGCA MamI 12824 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 13209 GATCCTCATC MluI 385 ACGCGT A^CGCGT 10100 ACGCGT NsiI 2312 ATGCAT ATGCA^T 9288 ATGCAT Psp1406I 984 AACGTT AA^CGTT 14361 AACGTT ######################################### # 3 OR MORE CUT ENZYMES ( 194 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspEI AspHI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NruI NspI NspV PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SmaI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI