##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I ApaI ApaLI
AscI Asp718I AspI AviII BamHI
BbrPI BcgI BcnI BfrI BglI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiWI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EcoNI EcoO65I EcoRI FauI
FseI FspI HapII HgaI HpaII
KpnI KspI MfeI MluI MroI
MspI MunI MvnI NaeI NciI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 1899 GGCGCC G[GA]^CG[CT]C
AhdI 943 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1751 GGATC GGATC(4/5)
AocI 1328 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2051 AGCGCT AGC^GCT
AspEI 943 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2169 GAGCTC G[AT]GC[AT]^C
BalI 517 TGGCCA TGG^CCA
BanI 1899 GGCGCC G^G[CT][GA]CC
BbeI 1899 GGCGCC GGCGC^C
BbvCI 905 CCTCAGC CCTCAGC(-5/-2)
BciVI 257 GTATCC GTATCC(6/5)
BglII 2898 AGATCT A^GATCT
BsaBI 1150 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1899 GGCGCC G[GA]^CG[CT]C
BsiEI 48 CGGCCG CG[GA][CT]^CG
BsiHKAI 2169 GAGCTC G[AT]GC[AT]^C
BsmBI 14 GAGACG CGTCTC(1/5)
BspMI 219 ACCTGC ACCTGC(4/8)
Bsu36I 1328 CCTAAGG CC^T[ACGT]AGG
DraIII 2794 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 442 CCATGG C^C[GA][CT]GG
EagI 48 CGGCCG C^GGCCG
Eam1105I 943 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 48 CGGCCG C^GGCCG
Eco47III 2051 AGCGCT AGC^GCT
Eco52I 48 CGGCCG C^GGCCG
Eco81I 1328 CCTAAGG CC^T[ACGT]AGG
Hin1I 1899 GGCGCC G[GA]^CG[CT]C
KasI 1899 GGCGCC G^GCGCC
MamI 1150 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 517 TGGCCA TGG^CCA
MscI 517 TGGCCA TGG^CCA
MspA1I 1955 CAGCTG C[AC]G^C[GT]G
NarI 1899 GGCGCC GG^CGCC
NcoI 442 CCATGG C^CATGG
NheI 3519 GCTAGC G^CTAGC
PflMI 632 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1955 CAGCTG CAG^CTG
SacI 2169 GAGCTC GAGCT^C
ScaI 110 AGTACT AGT^ACT
SfoI 1899 GGCGCC GGC^GCC
SpeI 2306 ACTAGT A^CTAGT
SphI 2841 GCATGC GCATG^C
Van91I 632 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 384 GTCTAC GT^[AC][GT]AC
1022 GTCTAC
AlwNI 1952 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3167 CAGATGCTG
AseI 3078 ATTAAT AT^TAAT
4034 ATTAAT
AsnI 3078 ATTAAT AT^TAAT
4034 ATTAAT
AvrII 2552 CCTAGG C^CTAGG
3907 CCTAGG
BanII 2169 GAGCTC G[GA]GC[CT]^C
3855 GAGCCC
BlnI 2552 CCTAGG C^CTAGG
3907 CCTAGG
BmyI 2169 GAGCTC G[AGT]GC[ACT]^C
3855 GAGCCC
BsgI 3173 CTGCAC GTGCAG(16/14)
2862 GTGCAG
BsmI 1348 GAATGC GAATGC(1/-1)
868 GCATTC
Bsp1286I 2169 GAGCTC G[AGT]GC[ACT]^C
3855 GAGCCC
BspHI 2432 TCATGA T^CATGA
3137 TCATGA
BspLU11I 1437 ACATGT A^CATGT
1711 ACATGT
BstAPI 1418 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3166 GCAGATGCTGC
BstYI 1751 GGATCT [GA]^GATC[CT]
2898 AGATCT
CfoI 1900 GCGC GCG^C
2052 GCGC
EaeI 48 CGGCCG [CT]^GGCC[GA]
517 TGGCCA
EcoRV 330 GATATC GAT^ATC
4053 GATATC
EcoT22I 2843 ATGCAT ATGCA^T
3683 ATGCAT
HaeII 1899 GGCGCC [GA]GCGC^[CT]
2051 AGCGCT
HhaI 1900 GCGC GCG^C
2052 GCGC
HinP1I 1900 GCGC G^CGC
2052 GCGC
HpaI 2545 GTTAAC GTT^AAC
2982 GTTAAC
MflI 1751 GGATCT [GA]^GATC[CT]
2898 AGATCT
NsiI 2843 ATGCAT ATGCA^T
3683 ATGCAT
PaeR7I 1527 CTCGAG C^TCGAG
2904 CTCGAG
PshBI 3078 ATTAAT AT^TAAT
4034 ATTAAT
PstI 1596 CTGCAG CTGCA^G
3989 CTGCAG
RcaI 2432 TCATGA T^CATGA
3137 TCATGA
XhoI 1527 CTCGAG C^TCGAG
2904 CTCGAG
XhoII 1751 GGATCT [GA]^GATC[CT]
2898 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI Asp700I AvaI AvaII BbsI
BbvI BclI BfaI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI