##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AgeI        AhdI        Aor51HI     AscI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BcgI        
BciVI       BlnI        BsaWI       BseAI       BsiEI       
BsiWI       BspDI       BspEI       BspHI       BssHII      
BstBI       BstUI       ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       FseI        FspI        HindIII     HpaI        
KpnI        KspI        MluI        MroI        MvnI        
NdeI        NotI        NruI        NspV        PacI        
PinAI       PmeI        Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflII        2664  CTTAAG               C^TTAAG
ApaI         2966  GGGCCC               GGGCC^C
AseI          882  ATTAAT               AT^TAAT
AsnI          882  ATTAAT               AT^TAAT
AspI         1398  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BclI         3517  TGATCA               T^GATCA
BfrI         2664  CTTAAG               C^TTAAG
BlpI         3046  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3046  GCTCAGC              GC^T[ACGT]AGC
BseRI        3375  GAGGAG               GAGGAG(10/8)
BsmBI        2438  CGTCTC               CGTCTC(1/5)
Bsp1407I      956  TGTACA               T^GTACA
BspLU11I     4084  ACATGT               A^CATGT
BsrDI        3774  CATTGC               GCAATG(2/0)
BsrGI         956  TGTACA               T^GTACA
BssSI        3080  CTCGTG               CACGAG(-5/-1)
Bst1107I      159  GTATAC               GTA^TAC
BstAPI        521  GCAGAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        371  GGTGACC              G^GT[ACGT]ACC
BstPI         371  GGTGACC              G^GT[ACGT]ACC
BstZ17I       159  GTATAC               GTA^TAC
CelII        3046  GCTCAGC              GC^T[ACGT]AGC
EcoO65I       371  GGTGACC              G^GT[ACGT]ACC
FbaI         3517  TGATCA               T^GATCA
MfeI         2806  CAATTG               C^AATTG
MunI         2806  CAATTG               C^AATTG
NaeI         2115  GCCGGC               GCC^GGC
NgoMIV       2115  GCCGGC               G^CCGGC
NheI         1599  GCTAGC               G^CTAGC
PaeR7I       4314  CTCGAG               C^TCGAG
PflFI        1398  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        4005  GACAGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         882  ATTAAT               AT^TAAT
ScaI         4348  AGTACT               AGT^ACT
SexAI        1813  ACCTGGT              A^CC[AT]GGT
SfiI           17  GGCCGCCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         4431  GCATGC               GCATG^C
SspBI         956  TGTACA               T^GTACA
SspI         1522  AATATT               AAT^ATT
StuI         4020  AGGCCT               AGG^CCT
Tth111I      1398  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4151  TCTAGA               T^CTAGA
XhoI         4314  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI          159  GTATAC               GT^[AC][GT]AC
             3560  GTCTAC              
AflIII       1852  ACGTGT               A^C[GA][CT]GT
             4084  ACATGT              
Alw44I       2556  GTGCAC               G^TGCAC
             4090  GTGCAC              
AocI          183  CCTGAGG              CC^T[ACGT]AGG
             2920  CCTCAGG             
ApaLI        2556  GTGCAC               G^TGCAC
             4090  GTGCAC              
BamHI        1490  GGATCC               G^GATCC
             3003  GGATCC              
BbeI          392  GGCGCC               GGCGC^C
             2399  GGCGCC              
BbrPI        2976  CACGTG               CAC^GTG
             3221  CACGTG              
BbsI         1745  GAAGAC               GAAGAC(2/6)
              732  GTCTTC              
BbvCI        1611  GCTGAGG              CCTCAGC(-5/-2)
             1677  GCTGAGG             
BglII        1882  AGATCT               A^GATCT
             3953  AGATCT              
BpuAI        1745  GAAGAC               GAAGAC(2/6)
              732  GTCTTC              
BsaBI        2018  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3423  GATGAATATC          
BsrBI        1904  CCGCTC               CCGCTC(-3/-3)
             1067  GAGCGG              
BsrFI          10  GCCGGT               [GA]^CCGG[CT]
             2115  GCCGGC              
Bsu36I        183  CCTGAGG              CC^T[ACGT]AGG
             2920  CCTCAGG             
Cfr10I         10  GCCGGT               [GA]^CCGG[CT]
             2115  GCCGGC              
DraI         3506  TTTAAA               TTT^AAA
             4174  TTTAAA              
DrdI           84  GACAACGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2908  GACCTCACAGTC        
Eco81I        183  CCTGAGG              CC^T[ACGT]AGG
             2920  CCTCAGG             
EcoNI         128  CCTCCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3995  CCTTCCCCAGG         
EcoRI         838  GAATTC               G^AATTC
             1895  GAATTC              
EcoT22I      3163  ATGCAT               ATGCA^T
             4188  ATGCAT              
HincII        246  GTTGAC               GT[CT]^[GA]AC
             2510  GTCAAC              
HindII        246  GTTGAC               GT[CT]^[GA]AC
             2510  GTCAAC              
KasI          392  GGCGCC               G^GCGCC
             2399  GGCGCC              
MamI         2018  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3423  GATGAATATC          
NarI          392  GGCGCC               GG^CGCC
             2399  GGCGCC              
NsiI         3163  ATGCAT               ATGCA^T
             4188  ATGCAT              
PmaCI        2976  CACGTG               CAC^GTG
             3221  CACGTG              
PmlI         2976  CACGTG               CAC^GTG
             3221  CACGTG              
SfoI          392  GGCGCC               GGC^GCC
             2399  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaAI       BsaHI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII