################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI AhdI Aor51HI AscI Asp700I Asp718I AspEI AviII AvrII BcgI BciVI BlnI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BssHII BstBI BstUI ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HindIII HpaI KpnI KspI MluI MroI MvnI NdeI NotI NruI NspV PacI PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflII 2664 CTTAAG C^TTAAG ApaI 2966 GGGCCC GGGCC^C AseI 882 ATTAAT AT^TAAT AsnI 882 ATTAAT AT^TAAT AspI 1398 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 3517 TGATCA T^GATCA BfrI 2664 CTTAAG C^TTAAG BlpI 3046 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3046 GCTCAGC GC^T[ACGT]AGC BseRI 3375 GAGGAG GAGGAG(10/8) BsmBI 2438 CGTCTC CGTCTC(1/5) Bsp1407I 956 TGTACA T^GTACA BspLU11I 4084 ACATGT A^CATGT BsrDI 3774 CATTGC GCAATG(2/0) BsrGI 956 TGTACA T^GTACA BssSI 3080 CTCGTG CACGAG(-5/-1) Bst1107I 159 GTATAC GTA^TAC BstAPI 521 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 371 GGTGACC G^GT[ACGT]ACC BstPI 371 GGTGACC G^GT[ACGT]ACC BstZ17I 159 GTATAC GTA^TAC CelII 3046 GCTCAGC GC^T[ACGT]AGC EcoO65I 371 GGTGACC G^GT[ACGT]ACC FbaI 3517 TGATCA T^GATCA MfeI 2806 CAATTG C^AATTG MunI 2806 CAATTG C^AATTG NaeI 2115 GCCGGC GCC^GGC NgoMIV 2115 GCCGGC G^CCGGC NheI 1599 GCTAGC G^CTAGC PaeR7I 4314 CTCGAG C^TCGAG PflFI 1398 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4005 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 882 ATTAAT AT^TAAT ScaI 4348 AGTACT AGT^ACT SexAI 1813 ACCTGGT A^CC[AT]GGT SfiI 17 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 4431 GCATGC GCATG^C SspBI 956 TGTACA T^GTACA SspI 1522 AATATT AAT^ATT StuI 4020 AGGCCT AGG^CCT Tth111I 1398 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4151 TCTAGA T^CTAGA XhoI 4314 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 159 GTATAC GT^[AC][GT]AC 3560 GTCTAC AflIII 1852 ACGTGT A^C[GA][CT]GT 4084 ACATGT Alw44I 2556 GTGCAC G^TGCAC 4090 GTGCAC AocI 183 CCTGAGG CC^T[ACGT]AGG 2920 CCTCAGG ApaLI 2556 GTGCAC G^TGCAC 4090 GTGCAC BamHI 1490 GGATCC G^GATCC 3003 GGATCC BbeI 392 GGCGCC GGCGC^C 2399 GGCGCC BbrPI 2976 CACGTG CAC^GTG 3221 CACGTG BbsI 1745 GAAGAC GAAGAC(2/6) 732 GTCTTC BbvCI 1611 GCTGAGG CCTCAGC(-5/-2) 1677 GCTGAGG BglII 1882 AGATCT A^GATCT 3953 AGATCT BpuAI 1745 GAAGAC GAAGAC(2/6) 732 GTCTTC BsaBI 2018 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3423 GATGAATATC BsrBI 1904 CCGCTC CCGCTC(-3/-3) 1067 GAGCGG BsrFI 10 GCCGGT [GA]^CCGG[CT] 2115 GCCGGC Bsu36I 183 CCTGAGG CC^T[ACGT]AGG 2920 CCTCAGG Cfr10I 10 GCCGGT [GA]^CCGG[CT] 2115 GCCGGC DraI 3506 TTTAAA TTT^AAA 4174 TTTAAA DrdI 84 GACAACGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2908 GACCTCACAGTC Eco81I 183 CCTGAGG CC^T[ACGT]AGG 2920 CCTCAGG EcoNI 128 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3995 CCTTCCCCAGG EcoRI 838 GAATTC G^AATTC 1895 GAATTC EcoT22I 3163 ATGCAT ATGCA^T 4188 ATGCAT HincII 246 GTTGAC GT[CT]^[GA]AC 2510 GTCAAC HindII 246 GTTGAC GT[CT]^[GA]AC 2510 GTCAAC KasI 392 GGCGCC G^GCGCC 2399 GGCGCC MamI 2018 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3423 GATGAATATC NarI 392 GGCGCC GG^CGCC 2399 GGCGCC NsiI 3163 ATGCAT ATGCA^T 4188 ATGCAT PmaCI 2976 CACGTG CAC^GTG 3221 CACGTG PmlI 2976 CACGTG CAC^GTG 3221 CACGTG SfoI 392 GGCGCC GGC^GCC 2399 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII