##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaLI       AscI        
Asp700I     AspEI       AviII       BamHI       BbrPI       
BcgI        BciVI       BclI        BfrI        BsaAI       
BsaWI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrGI       BssHII      
BssSI       BstBI       BstEII      BstPI       Bsu36I      
ClaI        CpoI        DrdI        Eam1105I    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        MfeI        
MluI        MroI        MunI        NaeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SwaI        XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I       1681  GGTACC               G^GTACC
AcyI          782  GGCGCC               G[GA]^CG[CT]C
AseI         3263  ATTAAT               AT^TAAT
AsnI         3263  ATTAAT               AT^TAAT
Asp718I      1681  GGTACC               G^GTACC
AspHI        4130  GTGCTC               G[AT]GC[AT]^C
BalI          911  TGGCCA               TGG^CCA
BbeI          782  GGCGCC               GGCGC^C
BsaHI         782  GGCGCC               G[GA]^CG[CT]C
BsiHKAI      4130  GTGCTC               G[AT]GC[AT]^C
BspLU11I     1021  ACATGT               A^CATGT
BsrBI         765  GAGCGG               CCGCTC(-3/-3)
BsrFI        1114  ACCGGC               [GA]^CCGG[CT]
Cfr10I       1114  ACCGGC               [GA]^CCGG[CT]
DraI         3976  TTTAAA               TTT^AAA
DraIII       2694  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Hin1I         782  GGCGCC               G[GA]^CG[CT]C
KasI          782  GGCGCC               G^GCGCC
KpnI         1681  GGTACC               GGTAC^C
KspI           31  CCGCGG               CCGC^GG
MluNI         911  TGGCCA               TGG^CCA
MscI          911  TGGCCA               TGG^CCA
NarI          782  GGCGCC               GG^CGCC
NdeI         3325  CATATG               CA^TATG
PshAI          41  GACCCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3263  ATTAAT               AT^TAAT
PstI         2655  CTGCAG               CTGCA^G
SacII          31  CCGCGG               CCGC^GG
ScaI         1963  AGTACT               AGT^ACT
SfoI          782  GGCGCC               GGC^GCC
TaqI         2119  TCGA                 T^CGA
TthHB8I      2119  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII          32  CGCG                 CG^CG
             1905  CGCG                
Aor51HI      1008  AGCGCT               AGC^GCT
             1687  AGCGCT              
ApaI         2956  GGGCCC               GGGCC^C
             3748  GGGCCC              
AspI           82  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              537  GACAGAGTC           
BbvCI        2590  CCTCAGC              CCTCAGC(-5/-2)
             3174  CCTCAGC             
BglII         709  AGATCT               A^GATCT
             3515  AGATCT              
BlpI          140  GCTTAGC              GC^T[ACGT]AGC
             2923  GCTGAGC             
Bpu1102I      140  GCTTAGC              GC^T[ACGT]AGC
             2923  GCTGAGC             
BsaBI        1652  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2534  GATCAGCATC          
BsiEI          34  CGGCCG               CG[GA][CT]^CG
              917  CGGCCG              
BsrDI         485  CATTGC               GCAATG(2/0)
             2420  CATTGC              
Bst1107I     2223  GTATAC               GTA^TAC
             3316  GTATAC              
BstAPI       1130  GCAGTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2648  GCAGGTGCTGC         
BstUI          32  CGCG                 CG^CG
             1905  CGCG                
BstZ17I      2223  GTATAC               GTA^TAC
             3316  GTATAC              
CelII         140  GCTTAGC              GC^T[ACGT]AGC
             2923  GCTGAGC             
EagI           34  CGGCCG               C^GGCCG
              917  CGGCCG              
EclXI          34  CGGCCG               C^GGCCG
              917  CGGCCG              
Eco47III     1008  AGCGCT               AGC^GCT
             1687  AGCGCT              
Eco52I         34  CGGCCG               C^GGCCG
              917  CGGCCG              
HgaI         1162  GCGTC                GACGC(5/10)
             4047  GCGTC               
HincII       1155  GTCAAC               GT[CT]^[GA]AC
             2447  GTTGAC              
HindII       1155  GTCAAC               GT[CT]^[GA]AC
             2447  GTTGAC              
MamI         1652  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2534  GATCAGCATC          
MvnI           32  CGCG                 CG^CG
             1905  CGCG                
NcoI         3083  CCATGG               C^CATGG
             3343  CCATGG              
PflFI          82  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              537  GACAGAGTC           
PflMI        1859  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4200  CCACATGCTGG         
SphI          506  GCATGC               GCATG^C
             1048  GCATGC              
SspI          553  AATATT               AAT^ATT
             4314  AATATT              
Tth111I        82  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              537  GACAGAGTC           
Van91I       1859  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4200  CCACATGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AvaI        
AvaII       AvrII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BlnI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       XmaCI       XmaI