##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AflII AhdI Alw44I
ApaLI Asp700I AspEI AspI AviII
BamHI BbvCI BfrI BglI BlpI
Bpu1102I BsaI BseAI BsiWI Bsp1407I
BspEI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
FseI FspI HindIII HpaI KspI
MluI MroI NaeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PmeI PvuI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI Sse8387I SspBI StuI
Tth111I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2221 GGTACC G^GTACC
AccI 1236 GTCGAC GT^[AC][GT]AC
AflIII 4112 ACATGT A^C[GA][CT]GT
AgeI 977 ACCGGT A^CCGGT
Aor51HI 875 AGCGCT AGC^GCT
AscI 66 GGCGCGCC GG^CGCGCC
AseI 1931 ATTAAT AT^TAAT
AsnI 1931 ATTAAT AT^TAAT
Asp718I 2221 GGTACC G^GTACC
AvrII 1269 CCTAGG C^CTAGG
BbrPI 402 CACGTG CAC^GTG
BcgI 2307 CGAAAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 518 TGATCA T^GATCA
BglII 3955 AGATCT A^GATCT
BlnI 1269 CCTAGG C^CTAGG
BsaBI 4195 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1257 GAGGAG GAGGAG(10/8)
BsiEI 98 CGACCG CG[GA][CT]^CG
BsmBI 178 CGTCTC CGTCTC(1/5)
BsmI 2564 GCATTC GAATGC(1/-1)
BspDI 4072 ATCGAT AT^CGAT
BspLU11I 4112 ACATGT A^CATGT
BsrDI 3645 CATTGC GCAATG(2/0)
BsrFI 977 ACCGGT [GA]^CCGG[CT]
BssSI 2018 CACGAG CACGAG(-5/-1)
Cfr10I 977 ACCGGT [GA]^CCGG[CT]
ClaI 4072 ATCGAT AT^CGAT
DraI 1722 TTTAAA TTT^AAA
DraIII 2776 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 875 AGCGCT AGC^GCT
EcoNI 1313 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2324 GAATTC G^AATTC
FbaI 518 TGATCA T^GATCA
KpnI 2221 GGTACC GGTAC^C
MamI 4195 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2760 CAATTG C^AATTG
MunI 2760 CAATTG C^AATTG
PaeR7I 1575 CTCGAG C^TCGAG
PinAI 977 ACCGGT A^CCGGT
PmaCI 402 CACGTG CAC^GTG
PmlI 402 CACGTG CAC^GTG
PshAI 1448 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1931 ATTAAT AT^TAAT
SacI 171 GAGCTC GAGCT^C
SalI 1236 GTCGAC G^TCGAC
SnaBI 2818 TACGTA TAC^GTA
SpeI 4259 ACTAGT A^CTAGT
SwaI 1721 ATTTAAAT ATTT^AAAT
XbaI 3905 TCTAGA T^CTAGA
XhoI 1575 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AclI 288 AACGTT AA^CGTT
1703 AACGTT
AcyI 724 GGCGCC G[GA]^CG[CT]C
3493 GGCGCC
AfaI 431 GTAC GT^AC
2222 GTAC
AocI 1278 CCTCAGG CC^T[ACGT]AGG
3335 CCTCAGG
AspHI 171 GAGCTC G[AT]GC[AT]^C
2046 GTGCTC
AvaI 463 CTCGGG C^[CT]CG[GA]G
1575 CTCGAG
BalI 394 TGGCCA TGG^CCA
3353 TGGCCA
BbeI 724 GGCGCC GGCGC^C
3493 GGCGCC
BbsI 335 GAAGAC GAAGAC(2/6)
1298 GTCTTC
BcnI 4 CCGGG CC^[GC]GG
150 CCCGG
BpuAI 335 GAAGAC GAAGAC(2/6)
1298 GTCTTC
BsaHI 724 GGCGCC G[GA]^CG[CT]C
3493 GGCGCC
BsaWI 977 ACCGGT [AT]^CCGG[AT]
1371 ACCGGA
BsgI 2117 CTGCAC GTGCAG(16/14)
1345 GTGCAG
BsiHKAI 171 GAGCTC G[AT]GC[AT]^C
2046 GTGCTC
BsoBI 463 CTCGGG C^[CT]CG[GA]G
1575 CTCGAG
BsrBI 142 CCGCTC CCGCTC(-3/-3)
2196 GAGCGG
BstYI 1913 GGATCT [GA]^GATC[CT]
3955 AGATCT
Bsu36I 1278 CCTCAGG CC^T[ACGT]AGG
3335 CCTCAGG
DrdI 1464 GACGCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3853 GACAGTTGAGTC
Eco81I 1278 CCTCAGG CC^T[ACGT]AGG
3335 CCTCAGG
EcoRV 1856 GATATC GAT^ATC
3023 GATATC
Hin1I 724 GGCGCC G[GA]^CG[CT]C
3493 GGCGCC
KasI 724 GGCGCC G^GCGCC
3493 GGCGCC
MflI 1913 GGATCT [GA]^GATC[CT]
3955 AGATCT
MluNI 394 TGGCCA TGG^CCA
3353 TGGCCA
MscI 394 TGGCCA TGG^CCA
3353 TGGCCA
NarI 724 GGCGCC GG^CGCC
3493 GGCGCC
NciI 4 CCGGG CC^[GC]GG
150 CCCGG
NdeI 544 CATATG CA^TATG
3885 CATATG
PflMI 2084 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4124 CCATAAAATGG
PpuMI 1570 AGGTCCT [GA]G^G[AT]CC[CT]
3272 AGGTCCC
Psp1406I 288 AACGTT AA^CGTT
1703 AACGTT
PstI 2173 CTGCAG CTGCA^G
3413 CTGCAG
PvuII 204 CAGCTG CAG^CTG
387 CAGCTG
RsaI 431 GTAC GT^AC
2222 GTAC
SfoI 724 GGCGCC GGC^GCC
3493 GGCGCC
SmlI 1575 CTCGAG C^T[CT][GA]AG
2300 CTCAAG
Van91I 2084 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4124 CCATAAAATGG
XhoII 1913 GGATCT [GA]^GATC[CT]
3955 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApaI ApoI AvaII BanI
BanII BbvI BciVI BfaI BmyI
BpmI BsaAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI RcaI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI