##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII BamHI BanI
BbeI BbrPI BciVI BglI BsaHI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT22I FseI FspI HgaI
Hin1I KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 1251 AACGTT AA^CGTT
AflIII 3194 ACGTGT A^C[GA][CT]GT
Asp700I 277 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4266 CCCGAG C^[CT]CG[GA]G
BalI 3905 TGGCCA TGG^CCA
BbvCI 7 GCTGAGG CCTCAGC(-5/-2)
BcgI 1667 CGACATATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 389 AGATCT A^GATCT
BsaAI 4451 TACGTG [CT]AC^GT[GA]
BsaWI 4103 ACCGGA [AT]^CCGG[AT]
BsmBI 150 CGTCTC CGTCTC(1/5)
BsoBI 4266 CCCGAG C^[CT]CG[GA]G
BsrFI 785 ACCGGC [GA]^CCGG[CT]
BstAPI 4007 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1443 GCGC GCG^C
Cfr10I 785 ACCGGC [GA]^CCGG[CT]
DraIII 1789 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1145 CCATGG C^C[GA][CT]GG
EcoNI 4262 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1195 GATATC GAT^ATC
FauI 4254 GCGGG CCCGC(4/6)
HaeII 1442 AGCGCC [GA]GCGC^[CT]
HhaI 1443 GCGC GCG^C
HinP1I 1443 GCGC G^CGC
HindIII 3707 AAGCTT A^AGCTT
MluNI 3905 TGGCCA TGG^CCA
MscI 3905 TGGCCA TGG^CCA
MslI 3956 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1145 CCATGG C^CATGG
Psp1406I 1251 AACGTT AA^CGTT
PstI 1948 CTGCAG CTGCA^G
SacI 4054 GAGCTC GAGCT^C
SapI 1038 GCTCTTC GCTCTTC(1/4)
SexAI 2601 ACCTGGT A^CC[AT]GGT
SpeI 3233 ACTAGT A^CTAGT
StuI 1415 AGGCCT AGG^CCT
XbaI 864 TCTAGA T^CTAGA
XmnI 277 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3866 GTCTAC GT^[AC][GT]AC
4154 GTAGAC
AciI 1514 GCGG CCGC(-3/-1)
4254 GCGG
AflII 2260 CTTAAG C^TTAAG
2590 CTTAAG
AlwI 2640 GATCC GGATC(4/5)
1085 GGATC
AspHI 4054 GAGCTC G[AT]GC[AT]^C
4434 GTGCTC
BbsI 2606 GTCTTC GAAGAC(2/6)
3281 GTCTTC
BcnI 1618 CCGGG CC^[GC]GG
2289 CCGGG
BfrI 2260 CTTAAG C^TTAAG
2590 CTTAAG
BlpI 3030 GCTTAGC GC^T[ACGT]AGC
4056 GCTCAGC
BpmI 1831 CTCCAG CTGGAG(16/14)
589 CTGGAG
Bpu1102I 3030 GCTTAGC GC^T[ACGT]AGC
4056 GCTCAGC
BpuAI 2606 GTCTTC GAAGAC(2/6)
3281 GTCTTC
BsaBI 504 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2064 GATAAAGATC
BsaI 3181 GAGACC GGTCTC(1/5)
1836 GGTCTC
BsiHKAI 4054 GAGCTC G[AT]GC[AT]^C
4434 GTGCTC
BsrDI 490 CATTGC GCAATG(2/0)
4012 CATTGC
BstYI 389 AGATCT [GA]^GATC[CT]
2639 AGATCC
CelII 3030 GCTTAGC GC^T[ACGT]AGC
4056 GCTCAGC
DraII 3445 AGGTCCT [GA]G^G[ACGT]CC[CT]
4256 GGGTCCC
DrdI 3272 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3857 GACTCTTGTGTC
EaeI 1663 TGGCCG [CT]^GGCC[GA]
3905 TGGCCA
EcoO109I 3445 AGGTCCT [GA]G^G[ACGT]CC[CT]
4256 GGGTCCC
MamI 504 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2064 GATAAAGATC
MflI 389 AGATCT [GA]^GATC[CT]
2639 AGATCC
NciI 1618 CCGGG CC^[GC]GG
2289 CCGGG
NdeI 1670 CATATG CA^TATG
2373 CATATG
PpuMI 3445 AGGTCCT [GA]G^G[AT]CC[CT]
4256 GGGTCCC
SphI 722 GCATGC GCATG^C
2741 GCATGC
SspI 1624 AATATT AAT^ATT
2412 AATATT
XcmI 572 CCATTTGGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2797 CCACCCTTGACTTGG
XhoII 389 AGATCT [GA]^GATC[CT]
2639 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI AvaII AvrII BanII BbvI
BclI BfaI BlnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BsrGI BsrI BssKI BstBI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MnlI
MseI MspA1I MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
NspV PleI PshBI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SfuI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I