##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       AclI        AgeI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        AviII       AvrII       BamHI       BbeI        
BbrPI       BlnI        BsiWI       BspDI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       FseI        FspI        
KasI        KspI        MfeI        MluI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SpeI        SwaI        XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        997  GGTACC               G^GTACC
AccIII       4009  TCCGGA               T^CCGGA
AcsI          378  AAATTC               [GA]^AATT[CT]
AflII        1734  CTTAAG               C^TTAAG
AhdI         3531  GACGAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3952  AGCGCT               AGC^GCT
ApoI          378  AAATTC               [GA]^AATT[CT]
Asp700I      1358  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       997  GGTACC               G^GTACC
AspEI        3531  GACGAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI        1761  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1389  GGATAC               GTATCC(6/5)
BclI         2191  TGATCA               T^GATCA
BfrI         1734  CTTAAG               C^TTAAG
BglI          345  GCCTTTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3749  AGATCT               A^GATCT
BlpI         3868  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3868  GCTCAGC              GC^T[ACGT]AGC
BsaBI         659  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        4009  TCCGGA               T^CCGGA
BsiEI        3897  CGGTCG               CG[GA][CT]^CG
BsmBI        3555  GAGACG               CGTCTC(1/5)
BspEI        4009  TCCGGA               T^CCGGA
BspLU11I     1108  ACATGT               A^CATGT
BssSI        2394  CTCGTG               CACGAG(-5/-1)
CelII        3868  GCTCAGC              GC^T[ACGT]AGC
DraI         3825  TTTAAA               TTT^AAA
Eam1105I     3531  GACGAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3952  AGCGCT               AGC^GCT
FauI         1269  GCGGG                CCCGC(4/6)
FbaI         2191  TGATCA               T^GATCA
HindIII      4090  AAGCTT               A^AGCTT
KpnI          997  GGTACC               GGTAC^C
MamI          659  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         4009  TCCGGA               T^CCGGA
NaeI         2989  GCCGGC               GCC^GGC
NcoI         1468  CCATGG               C^CATGG
NgoMIV       2989  GCCGGC               G^CCGGC
PshAI         612  GACATCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1245  CAGCTG               CAG^CTG
SacI         3620  GAGCTC               GAGCT^C
SbfI         2945  CCTGCAGG             CCTGCA^GG
ScaI         3098  AGTACT               AGT^ACT
SfiI          344  GGCCTTTGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI        2642  TACGTA               TAC^GTA
SphI         2563  GCATGC               GCATG^C
Sse8387I     2945  CCTGCAGG             CCTGCA^GG
SspI         3129  AATATT               AAT^ATT
StuI         3990  AGGCCT               AGG^CCT
XmnI         1358  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          885  GTAGAC               GT^[AC][GT]AC
             3218  GTCTAC              
AcyI         2449  GACGCC               G[GA]^CG[CT]C
             3049  GACGCC              
AflIII       1108  ACATGT               A^C[GA][CT]GT
             3670  ACGTGT              
AspHI        2779  GTGCTC               G[AT]GC[AT]^C
             3620  GAGCTC              
AspI          282  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1983  GACCCTGTC           
BcgI         3196  CGACCTCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1700  GCACACCGTTCG        
BfaI         2427  CTAG                 C^TAG
             3994  CTAG                
BsaHI        2449  GACGCC               G[GA]^CG[CT]C
             3049  GACGCC              
BsaWI        1386  ACCGGA               [AT]^CCGG[AT]
             4009  TCCGGA              
BsiHKAI      2779  GTGCTC               G[AT]GC[AT]^C
             3620  GAGCTC              
BspHI        1822  TCATGA               T^CATGA
             3601  TCATGA              
BsrBI        2486  GAGCGG               CCGCTC(-3/-3)
             3629  GAGCGG              
BsrFI        1380  ACCGGC               [GA]^CCGG[CT]
             2989  GCCGGC              
BstYI        1087  AGATCC               [GA]^GATC[CT]
             3749  AGATCT              
Cfr10I       1380  ACCGGC               [GA]^CCGG[CT]
             2989  GCCGGC              
EcoT22I      1476  ATGCAT               ATGCA^T
             3851  ATGCAT              
HaeII        2637  GGCGCT               [GA]GCGC^[CT]
             3952  AGCGCT              
Hin1I        2449  GACGCC               G[GA]^CG[CT]C
             3049  GACGCC              
MaeI         2427  CTAG                 C^TAG
             3994  CTAG                
MflI         1087  AGATCC               [GA]^GATC[CT]
             3749  AGATCT              
NsiI         1476  ATGCAT               ATGCA^T
             3851  ATGCAT              
PflFI         282  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1983  GACCCTGTC           
PflMI         366  CCAGTGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2784  CCAGGACTTGG         
RcaI         1822  TCATGA               T^CATGA
             3601  TCATGA              
SapI         3626  GAAGAGC              GCTCTTC(1/4)
             2475  GCTCTTC             
Tth111I       282  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1983  GACCCTGTC           
Van91I        366  CCAGTGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2784  CCAGGACTTGG         
XhoII        1087  AGATCC               [GA]^GATC[CT]
             3749  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrDI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaI        HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PaeR7I      PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoI