################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI Asp718I AviII AvrII BfrI BlnI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI Eco47III EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI MfeI MroI MunI NheI NotI NruI NsiI NspI NspV PinAI PmeI PshAI Psp1406I PvuI RsrII SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 740 GACGTC GACGT^C AccI 324 GTCGAC GT^[AC][GT]AC AcsI 3077 GAATTT [GA]^AATT[CT] AfaI 3350 GTAC GT^AC AhdI 452 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1617 CCTTAGG CC^T[ACGT]AGG ApoI 3077 GAATTT [GA]^AATT[CT] AscI 1683 GGCGCGCC GG^CGCGCC Asp700I 1890 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 452 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1934 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 831 GCAAGGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1752 GTATCC GTATCC(6/5) BsaBI 2537 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1325 GTGCAG GTGCAG(16/14) BsmBI 2654 CGTCTC CGTCTC(1/5) BspHI 2782 TCATGA T^CATGA BstAPI 1327 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3131 GGTCACC G^GT[ACGT]ACC BstPI 3131 GGTCACC G^GT[ACGT]ACC Bsu36I 1617 CCTTAGG CC^T[ACGT]AGG DraIII 2296 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3382 GACACCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 452 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1617 CCTTAGG CC^T[ACGT]AGG EcoO65I 3131 GGTCACC G^GT[ACGT]ACC HindIII 3617 AAGCTT A^AGCTT KspI 1480 CCGCGG CCGC^GG MamI 2537 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1351 ACGCGT A^CGCGT NcoI 63 CCATGG C^CATGG NdeI 2190 CATATG CA^TATG PacI 2239 TTAATTAA TTAAT^TAA PaeR7I 1012 CTCGAG C^TCGAG PflFI 1934 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 173 CTGCAG CTGCA^G RcaI 2782 TCATGA T^CATGA RsaI 3350 GTAC GT^AC SacII 1480 CCGCGG CCGC^GG SalI 324 GTCGAC G^TCGAC SapI 2276 GCTCTTC GCTCTTC(1/4) SexAI 2427 ACCTGGT A^CC[AT]GGT Tth111I 1934 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3325 TCTAGA T^CTAGA XhoI 1012 CTCGAG C^TCGAG XmnI 1890 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 110 ACGTGT A^C[GA][CT]GT 1351 ACGCGT AseI 2199 ATTAAT AT^TAAT 2242 ATTAAT AsnI 2199 ATTAAT AT^TAAT 2242 ATTAAT BalI 3027 TGGCCA TGG^CCA 3458 TGGCCA BbeI 406 GGCGCC GGCGC^C 1850 GGCGCC BbrPI 109 CACGTG CAC^GTG 1273 CACGTG BbsI 16 GAAGAC GAAGAC(2/6) 1915 GAAGAC BbvCI 1493 GCTGAGG CCTCAGC(-5/-2) 1793 GCTGAGG BclI 2441 TGATCA T^GATCA 2785 TGATCA BglII 1038 AGATCT A^GATCT 2670 AGATCT BlpI 1801 GCTCAGC GC^T[ACGT]AGC 2309 GCTGAGC Bpu1102I 1801 GCTCAGC GC^T[ACGT]AGC 2309 GCTGAGC BpuAI 16 GAAGAC GAAGAC(2/6) 1915 GAAGAC BsaWI 1130 ACCGGA [AT]^CCGG[AT] 1171 ACCGGA CelII 1801 GCTCAGC GC^T[ACGT]AGC 2309 GCTGAGC EcoNI 205 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1876 CCTGCCCGAGG FbaI 2441 TGATCA T^GATCA 2785 TGATCA HincII 324 GTCGAC GT[CT]^[GA]AC 2570 GTTGAC HindII 324 GTCGAC GT[CT]^[GA]AC 2570 GTTGAC KasI 406 GGCGCC G^GCGCC 1850 GGCGCC MluNI 3027 TGGCCA TGG^CCA 3458 TGGCCA MscI 3027 TGGCCA TGG^CCA 3458 TGGCCA NarI 406 GGCGCC GG^CGCC 1850 GGCGCC PmaCI 109 CACGTG CAC^GTG 1273 CACGTG PmlI 109 CACGTG CAC^GTG 1273 CACGTG PshBI 2199 ATTAAT AT^TAAT 2242 ATTAAT PvuII 365 CAGCTG CAG^CTG 672 CAGCTG SfoI 406 GGCGCC GGC^GCC 1850 GGCGCC SmlI 1004 CTCAAG C^T[CT][GA]AG 1012 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI