##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaLI       Asp718I     AviII       
AvrII       BfrI        BlnI        BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstXI       BstZ17I     ClaI        CpoI        DraI        
Eco47III    EcoRI       EcoRV       EcoT22I     FseI        
FspI        HpaI        KpnI        MfeI        MroI        
MunI        NheI        NotI        NruI        NsiI        
NspI        NspV        PinAI       PmeI        PshAI       
Psp1406I    PvuI        RsrII       SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         740  GACGTC               GACGT^C
AccI          324  GTCGAC               GT^[AC][GT]AC
AcsI         3077  GAATTT               [GA]^AATT[CT]
AfaI         3350  GTAC                 GT^AC
AhdI          452  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1617  CCTTAGG              CC^T[ACGT]AGG
ApoI         3077  GAATTT               [GA]^AATT[CT]
AscI         1683  GGCGCGCC             GG^CGCGCC
Asp700I      1890  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         452  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1934  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI          831  GCAAGGGGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1752  GTATCC               GTATCC(6/5)
BsaBI        2537  GATTTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1325  GTGCAG               GTGCAG(16/14)
BsmBI        2654  CGTCTC               CGTCTC(1/5)
BspHI        2782  TCATGA               T^CATGA
BstAPI       1327  GCAGCTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3131  GGTCACC              G^GT[ACGT]ACC
BstPI        3131  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1617  CCTTAGG              CC^T[ACGT]AGG
DraIII       2296  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3382  GACACCGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      452  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1617  CCTTAGG              CC^T[ACGT]AGG
EcoO65I      3131  GGTCACC              G^GT[ACGT]ACC
HindIII      3617  AAGCTT               A^AGCTT
KspI         1480  CCGCGG               CCGC^GG
MamI         2537  GATTTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1351  ACGCGT               A^CGCGT
NcoI           63  CCATGG               C^CATGG
NdeI         2190  CATATG               CA^TATG
PacI         2239  TTAATTAA             TTAAT^TAA
PaeR7I       1012  CTCGAG               C^TCGAG
PflFI        1934  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          173  CTGCAG               CTGCA^G
RcaI         2782  TCATGA               T^CATGA
RsaI         3350  GTAC                 GT^AC
SacII        1480  CCGCGG               CCGC^GG
SalI          324  GTCGAC               G^TCGAC
SapI         2276  GCTCTTC              GCTCTTC(1/4)
SexAI        2427  ACCTGGT              A^CC[AT]GGT
Tth111I      1934  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3325  TCTAGA               T^CTAGA
XhoI         1012  CTCGAG               C^TCGAG
XmnI         1890  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflIII        110  ACGTGT               A^C[GA][CT]GT
             1351  ACGCGT              
AseI         2199  ATTAAT               AT^TAAT
             2242  ATTAAT              
AsnI         2199  ATTAAT               AT^TAAT
             2242  ATTAAT              
BalI         3027  TGGCCA               TGG^CCA
             3458  TGGCCA              
BbeI          406  GGCGCC               GGCGC^C
             1850  GGCGCC              
BbrPI         109  CACGTG               CAC^GTG
             1273  CACGTG              
BbsI           16  GAAGAC               GAAGAC(2/6)
             1915  GAAGAC              
BbvCI        1493  GCTGAGG              CCTCAGC(-5/-2)
             1793  GCTGAGG             
BclI         2441  TGATCA               T^GATCA
             2785  TGATCA              
BglII        1038  AGATCT               A^GATCT
             2670  AGATCT              
BlpI         1801  GCTCAGC              GC^T[ACGT]AGC
             2309  GCTGAGC             
Bpu1102I     1801  GCTCAGC              GC^T[ACGT]AGC
             2309  GCTGAGC             
BpuAI          16  GAAGAC               GAAGAC(2/6)
             1915  GAAGAC              
BsaWI        1130  ACCGGA               [AT]^CCGG[AT]
             1171  ACCGGA              
CelII        1801  GCTCAGC              GC^T[ACGT]AGC
             2309  GCTGAGC             
EcoNI         205  CCTGGCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1876  CCTGCCCGAGG         
FbaI         2441  TGATCA               T^GATCA
             2785  TGATCA              
HincII        324  GTCGAC               GT[CT]^[GA]AC
             2570  GTTGAC              
HindII        324  GTCGAC               GT[CT]^[GA]AC
             2570  GTTGAC              
KasI          406  GGCGCC               G^GCGCC
             1850  GGCGCC              
MluNI        3027  TGGCCA               TGG^CCA
             3458  TGGCCA              
MscI         3027  TGGCCA               TGG^CCA
             3458  TGGCCA              
NarI          406  GGCGCC               GG^CGCC
             1850  GGCGCC              
PmaCI         109  CACGTG               CAC^GTG
             1273  CACGTG              
PmlI          109  CACGTG               CAC^GTG
             1273  CACGTG              
PshBI        2199  ATTAAT               AT^TAAT
             2242  ATTAAT              
PvuII         365  CAGCTG               CAG^CTG
              672  CAGCTG              
SfoI          406  GGCGCC               GGC^GCC
             1850  GGCGCC              
SmlI         1004  CTCAAG               C^T[CT][GA]AG
             1012  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PflMI       PleI        
PpuMI       SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI