##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AflIII AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BamHI BbsI BbvCI BclI BlnI
BpuAI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssSI BstBI BstEII BstPI
BstXI ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoNI EcoO65I FbaI
FseI FspI HincII HindII HpaI
MamI MluI MroI NdeI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PvuI PvuII
RsrII SalI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI StuI SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3696 GTATAC GT^[AC][GT]AC
AclI 2087 AACGTT AA^CGTT
Alw44I 2485 GTGCAC G^TGCAC
AocI 594 CCTAAGG CC^T[ACGT]AGG
ApaLI 2485 GTGCAC G^TGCAC
AseI 499 ATTAAT AT^TAAT
AsnI 499 ATTAAT AT^TAAT
AspI 2317 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2710 CACGTG CAC^GTG
BcgI 60 GCAGGCGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1617 GTATCC GTATCC(6/5)
BlpI 3914 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3914 GCTGAGC GC^T[ACGT]AGC
BsaAI 2710 CACGTG [CT]AC^GT[GA]
BsmBI 2498 GAGACG CGTCTC(1/5)
BspMI 2540 ACCTGC ACCTGC(4/8)
BsrFI 109 GCCGGC [GA]^CCGG[CT]
Bst1107I 3696 GTATAC GTA^TAC
BstZ17I 3696 GTATAC GTA^TAC
Bsu36I 594 CCTAAGG CC^T[ACGT]AGG
CelII 3914 GCTGAGC GC^T[ACGT]AGC
Cfr10I 109 GCCGGC [GA]^CCGG[CT]
EagI 93 CGGCCG C^GGCCG
EclXI 93 CGGCCG C^GGCCG
Eco52I 93 CGGCCG C^GGCCG
Eco81I 594 CCTAAGG CC^T[ACGT]AGG
EcoRV 714 GATATC GAT^ATC
HindIII 2520 AAGCTT A^AGCTT
KspI 309 CCGCGG CCGC^GG
NaeI 109 GCCGGC GCC^GGC
NgoMIV 109 GCCGGC G^CCGGC
NheI 289 GCTAGC G^CTAGC
PflFI 2317 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2710 CACGTG CAC^GTG
PmlI 2710 CACGTG CAC^GTG
PshBI 499 ATTAAT AT^TAAT
Psp1406I 2087 AACGTT AA^CGTT
SacI 1463 GAGCTC GAGCT^C
SacII 309 CCGCGG CCGC^GG
ScaI 2003 AGTACT AGT^ACT
SgrAI 108 CGCCGGCG C[GA]^CCGG[CT]G
Tth111I 2317 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2402 CCAAAGGTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 821 GGTACC G^GTACC
1048 GGTACC
AflII 1009 CTTAAG C^TTAAG
3788 CTTAAG
AlwNI 1947 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
3134 CAGATGCTG
Asp700I 1228 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2123 GAATTGATTC
Asp718I 821 GGTACC G^GTACC
1048 GGTACC
AspHI 1463 GAGCTC G[AT]GC[AT]^C
2485 GTGCAC
BfrI 1009 CTTAAG C^TTAAG
3788 CTTAAG
BglI 95 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
109 GCCGGCGGGGC
BglII 2874 AGATCT A^GATCT
3294 AGATCT
BpmI 2675 CTGGAG CTGGAG(16/14)
3455 CTGGAG
BsaI 2289 GGTCTC GGTCTC(1/5)
2331 GGTCTC
BsgI 58 GTGCAG GTGCAG(16/14)
2462 GTGCAG
BsiEI 93 CGGCCG CG[GA][CT]^CG
341 CGGTCG
BsiHKAI 1463 GAGCTC G[AT]GC[AT]^C
2485 GTGCAC
BspHI 1086 TCATGA T^CATGA
2276 TCATGA
BsrDI 695 GCAATG GCAATG(2/0)
960 GCAATG
BssHII 3 GCGCGC G^CGCGC
220 GCGCGC
BstAPI 787 GCAGATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1600 GCATTCTTTGC
DraI 987 TTTAAA TTT^AAA
3568 TTTAAA
EarI 423 GAAGAG CTCTTC(1/4)
2729 GAAGAG
EcoT22I 1598 ATGCAT ATGCA^T
2795 ATGCAT
KpnI 821 GGTACC GGTAC^C
1048 GGTACC
Ksp632I 423 GAAGAG CTCTTC(1/4)
2729 GAAGAG
MaeII 2088 ACGT A^CGT
2711 ACGT
MfeI 3509 CAATTG C^AATTG
3999 CAATTG
MspA1I 231 CAGCGG C[AC]G^C[GT]G
309 CCGCGG
MunI 3509 CAATTG C^AATTG
3999 CAATTG
NsiI 1598 ATGCAT ATGCA^T
2795 ATGCAT
RcaI 1086 TCATGA T^CATGA
2276 TCATGA
SphI 458 GCATGC GCATG^C
3906 GCATGC
XbaI 547 TCTAGA T^CTAGA
3889 TCTAGA
XmnI 1228 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2123 GAATTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BmyI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI