##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII BciVI
BfrI BglII BsaBI BsiEI BsiWI
BsmBI BspDI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI FspI HpaI KpnI
MamI MfeI MluI MunI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 783 TCCGGA T^CCGGA
Aor51HI 202 AGCGCT AGC^GCT
BbeI 156 GGCGCC GGCGC^C
BbrPI 2011 CACGTG CAC^GTG
BbsI 3704 GAAGAC GAAGAC(2/6)
BcgI 1375 CGACGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 3704 GAAGAC GAAGAC(2/6)
BsaAI 2011 CACGTG [CT]AC^GT[GA]
BseAI 783 TCCGGA T^CCGGA
BsgI 2809 GTGCAG GTGCAG(16/14)
BsmI 2457 GCATTC GAATGC(1/-1)
Bsp1407I 1151 TGTACA T^GTACA
BspEI 783 TCCGGA T^CCGGA
BspHI 577 TCATGA T^CATGA
BsrGI 1151 TGTACA T^GTACA
DraI 3977 TTTAAA TTT^AAA
DraIII 3715 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1975 GACTCCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 202 AGCGCT AGC^GCT
HincII 1726 GTCAAC GT[CT]^[GA]AC
HindII 1726 GTCAAC GT[CT]^[GA]AC
HindIII 2725 AAGCTT A^AGCTT
KasI 156 GGCGCC G^GCGCC
KspI 2247 CCGCGG CCGC^GG
MroI 783 TCCGGA T^CCGGA
NaeI 2521 GCCGGC GCC^GGC
NarI 156 GGCGCC GG^CGCC
NdeI 1999 CATATG CA^TATG
NgoMIV 2521 GCCGGC G^CCGGC
PaeR7I 1180 CTCGAG C^TCGAG
PmaCI 2011 CACGTG CAC^GTG
PmlI 2011 CACGTG CAC^GTG
PshAI 3059 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 577 TCATGA T^CATGA
SacII 2247 CCGCGG CCGC^GG
ScaI 466 AGTACT AGT^ACT
SfiI 2217 GGCCAAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 156 GGCGCC GGC^GCC
SspBI 1151 TGTACA T^GTACA
SspI 402 AATATT AAT^ATT
TfiI 69 GATTC G^A[AT]TC
XhoI 1180 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1963 GTCTAC GT^[AC][GT]AC
1984 GTCTAC
AccII 1960 CGCG CG^CG
2248 CGCG
AlwNI 231 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
3151 CAGGAGCTG
Asp700I 991 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3082 GAAGCCATTC
BclI 806 TGATCA T^GATCA
1736 TGATCA
BlpI 3156 GCTGAGC GC^T[ACGT]AGC
3552 GCTGAGC
Bpu1102I 3156 GCTGAGC GC^T[ACGT]AGC
3552 GCTGAGC
BsaWI 783 TCCGGA [AT]^CCGG[AT]
1274 ACCGGA
BsrDI 668 CATTGC GCAATG(2/0)
151 GCAATG
BsrFI 1679 ACCGGC [GA]^CCGG[CT]
2521 GCCGGC
BstAPI 316 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2833 GCAGCACTTGC
BstEII 2056 GGTGACC G^GT[ACGT]ACC
2439 GGTGACC
BstPI 2056 GGTGACC G^GT[ACGT]ACC
2439 GGTGACC
BstUI 1960 CGCG CG^CG
2248 CGCG
CelII 3156 GCTGAGC GC^T[ACGT]AGC
3552 GCTGAGC
Cfr10I 1679 ACCGGC [GA]^CCGG[CT]
2521 GCCGGC
EcoO65I 2056 GGTGACC G^GT[ACGT]ACC
2439 GGTGACC
EcoRI 630 GAATTC G^AATTC
2472 GAATTC
FbaI 806 TGATCA T^GATCA
1736 TGATCA
MvnI 1960 CGCG CG^CG
2248 CGCG
NheI 2859 GCTAGC G^CTAGC
3633 GCTAGC
NspI 316 GCATGT [GA]CATG^[CT]
3557 GCATGT
PstI 3004 CTGCAG CTGCA^G
3070 CTGCAG
PvuII 35 CAGCTG CAG^CTG
1618 CAGCTG
SacI 790 GAGCTC GAGCT^C
2280 GAGCTC
SfcI 3004 CTGCAG C^T[GA][CT]AG
3070 CTGCAG
TaqI 130 TCGA T^CGA
1181 TCGA
TthHB8I 130 TCGA T^CGA
1181 TCGA
XmnI 991 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3082 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AocI ApaI ApaLI
ApoI AspHI AvaI AvaII AvrII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
SmlI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI