##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AhdI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BcgI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BssSI Bst1107I BstAPI
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoRV FseI FspI Hin1I HpaI
KasI MamI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfoI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 3394 GGTACC G^GTACC
AflII 3389 CTTAAG C^TTAAG
AflIII 339 ACATGT A^C[GA][CT]GT
AgeI 1732 ACCGGT A^CCGGT
Alw44I 473 GTGCAC G^TGCAC
AlwNI 1437 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3438 CCTGAGG CC^T[ACGT]AGG
Aor51HI 30 AGCGCT AGC^GCT
ApaI 1304 GGGCCC GGGCC^C
ApaLI 473 GTGCAC G^TGCAC
Asp718I 3394 GGTACC G^GTACC
BalI 751 TGGCCA TGG^CCA
BamHI 991 GGATCC G^GATCC
BanI 3394 GGTACC G^G[CT][GA]CC
BbvCI 1268 CCTCAGC CCTCAGC(-5/-2)
BciVI 1220 GTATCC GTATCC(6/5)
BcnI 1358 CCGGG CC^[GC]GG
BfrI 3389 CTTAAG C^TTAAG
BglI 1316 GCCCACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1912 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1912 GCTAAGC GC^T[ACGT]AGC
BsaI 697 GAGACC GGTCTC(1/5)
BsaWI 1732 ACCGGT [AT]^CCGG[AT]
BspLU11I 339 ACATGT A^CATGT
BsrBI 1535 CCGCTC CCGCTC(-3/-3)
BsrFI 1732 ACCGGT [GA]^CCGG[CT]
BssHII 4 GCGCGC G^CGCGC
BstBI 1282 TTCGAA TT^CGAA
Bsu36I 3438 CCTGAGG CC^T[ACGT]AGG
CelII 1912 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1732 ACCGGT [GA]^CCGG[CT]
EaeI 751 TGGCCA [CT]^GGCC[GA]
Eco47III 30 AGCGCT AGC^GCT
Eco81I 3438 CCTGAGG CC^T[ACGT]AGG
HaeII 30 AGCGCT [GA]GCGC^[CT]
HgaI 272 GACGC GACGC(5/10)
HincII 1244 GTTGAC GT[CT]^[GA]AC
HindII 1244 GTTGAC GT[CT]^[GA]AC
HindIII 154 AAGCTT A^AGCTT
KpnI 3394 GGTACC GGTAC^C
KspI 9 CCGCGG CCGC^GG
MluNI 751 TGGCCA TGG^CCA
MscI 751 TGGCCA TGG^CCA
NciI 1358 CCGGG CC^[GC]GG
NdeI 2659 CATATG CA^TATG
NheI 3478 GCTAGC G^CTAGC
NspV 1282 TTCGAA TT^CGAA
PinAI 1732 ACCGGT A^CCGGT
PleI 491 GAGTC GAGTC(4/5)
SacI 3758 GAGCTC GAGCT^C
SacII 9 CCGCGG CCGC^GG
SapI 1133 GAAGAGC GCTCTTC(1/4)
SexAI 2602 ACCTGGT A^CC[AT]GGT
SfiI 1315 GGCCCACTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1282 TTCGAA TT^CGAA
SpeI 2473 ACTAGT A^CTAGT
SspI 2391 AATATT AAT^ATT
TaqI 1283 TCGA T^CGA
TthHB8I 1283 TCGA T^CGA
XbaI 4021 TCTAGA T^CTAGA
XcmI 388 CCAGTCCTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 5 CGCG CG^CG
10 CGCG
BclI 1275 TGATCA T^GATCA
2456 TGATCA
BpmI 246 CTCCAG CTGGAG(16/14)
1484 CTCCAG
Bsp1407I 1868 TGTACA T^GTACA
3677 TGTACA
BspMI 2886 ACCTGC ACCTGC(4/8)
1576 GCAGGT
BsrDI 816 GCAATG GCAATG(2/0)
1688 GCAATG
BsrGI 1868 TGTACA T^GTACA
3677 TGTACA
BstEII 497 GGTTACC G^GT[ACGT]ACC
1942 GGTCACC
BstPI 497 GGTTACC G^GT[ACGT]ACC
1942 GGTCACC
BstUI 5 CGCG CG^CG
10 CGCG
BstXI 585 CCAATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3536 CCAAGAGAATGG
BstYI 991 GGATCC [GA]^GATC[CT]
3724 AGATCC
DraII 286 GGGACCT [GA]G^G[ACGT]CC[CT]
3050 AGGTCCT
EcoO109I 286 GGGACCT [GA]G^G[ACGT]CC[CT]
3050 AGGTCCT
EcoO65I 497 GGTTACC G^GT[ACGT]ACC
1942 GGTCACC
EcoRI 1834 GAATTC G^AATTC
3363 GAATTC
EcoT22I 1385 ATGCAT ATGCA^T
3608 ATGCAT
FbaI 1275 TGATCA T^GATCA
2456 TGATCA
HapII 1358 CCGG C^CGG
1733 CCGG
HpaII 1358 CCGG C^CGG
1733 CCGG
MaeII 228 ACGT A^CGT
1248 ACGT
MflI 991 GGATCC [GA]^GATC[CT]
3724 AGATCC
MspI 1358 CCGG C^CGG
1733 CCGG
MvnI 5 CGCG CG^CG
10 CGCG
NcoI 1300 CCATGG C^CATGG
1855 CCATGG
NsiI 1385 ATGCAT ATGCA^T
3608 ATGCAT
PflMI 463 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1684 CCAGGCAATGG
PpuMI 286 GGGACCT [GA]G^G[AT]CC[CT]
3050 AGGTCCT
SphI 1770 GCATGC GCATG^C
3166 GCATGC
SspBI 1868 TGTACA T^GTACA
3677 TGTACA
Van91I 463 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1684 CCAGGCAATGG
XhoII 991 GGATCC [GA]^GATC[CT]
3724 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI