##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspI AviII BamHI BbeI BbrPI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI EcoO65I
FspI HincII HindII HpaI KasI
KspI MamI MluI MroI NarI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1732 GACGTC GACGT^C
AflIII 86 ACATGT A^C[GA][CT]GT
AhdI 329 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2835 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2303 AGCGCT AGC^GCT
AspEI 329 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 156 CTCGGG C^[CT]CG[GA]G
BcgI 997 CGATGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2297 GTATCC GTATCC(6/5)
BclI 1861 TGATCA T^GATCA
BglI 1223 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 3789 GGTCTC GGTCTC(1/5)
BsgI 2501 CTGCAC GTGCAG(16/14)
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsoBI 156 CTCGGG C^[CT]CG[GA]G
BspHI 655 TCATGA T^CATGA
BspLU11I 86 ACATGT A^CATGT
Bsu36I 2835 CCTGAGG CC^T[ACGT]AGG
DrdI 3347 GACTCCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2 CGGCCG C^GGCCG
Eam1105I 329 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2 CGGCCG C^GGCCG
Eco47III 2303 AGCGCT AGC^GCT
Eco52I 2 CGGCCG C^GGCCG
Eco81I 2835 CCTGAGG CC^T[ACGT]AGG
EcoRI 863 GAATTC G^AATTC
EcoRV 884 GATATC GAT^ATC
FbaI 1861 TGATCA T^GATCA
FseI 3 GGCCGGCC GGCCGG^CC
HaeII 2303 AGCGCT [GA]GCGC^[CT]
HindIII 3630 AAGCTT A^AGCTT
MfeI 829 CAATTG C^AATTG
MunI 829 CAATTG C^AATTG
NdeI 2452 CATATG CA^TATG
PflMI 2710 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3519 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 655 TCATGA T^CATGA
SexAI 2792 ACCTGGT A^CC[AT]GGT
SfiI 1222 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Van91I 2710 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1706 GGTACC G^GTACC
3326 GGTACC
AcyI 1732 GACGTC G[GA]^CG[CT]C
2681 GGCGTC
Asp718I 1706 GGTACC G^GTACC
3326 GGTACC
AvrII 3642 CCTAGG C^CTAGG
3815 CCTAGG
BcnI 1204 CCGGG CC^[GC]GG
2678 CCCGG
BlnI 3642 CCTAGG C^CTAGG
3815 CCTAGG
BsaHI 1732 GACGTC G[GA]^CG[CT]C
2681 GGCGTC
BsmBI 1904 CGTCTC CGTCTC(1/5)
1519 GAGACG
BsrDI 809 CATTGC GCAATG(2/0)
3390 CATTGC
BsrFI 4 GCCGGC [GA]^CCGG[CT]
43 GCCGGC
BstYI 2110 AGATCC [GA]^GATC[CT]
3413 AGATCC
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
43 GCCGGC
DraIII 2247 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3359 CACACTGTG
Hin1I 1732 GACGTC G[GA]^CG[CT]C
2681 GGCGTC
KpnI 1706 GGTACC GGTAC^C
3326 GGTACC
MaeII 1549 ACGT A^CGT
1733 ACGT
MflI 2110 AGATCC [GA]^GATC[CT]
3413 AGATCC
NaeI 4 GCCGGC GCC^GGC
43 GCCGGC
NciI 1204 CCGGG CC^[GC]GG
2678 CCCGG
NcoI 147 CCATGG C^CATGG
1369 CCATGG
NgoMIV 4 GCCGGC G^CCGGC
43 GCCGGC
NheI 1046 GCTAGC G^CTAGC
1987 GCTAGC
SapI 2057 GAAGAGC GCTCTTC(1/4)
1965 GCTCTTC
ScaI 1059 AGTACT AGT^ACT
3252 AGTACT
SphI 2447 GCATGC GCATG^C
3374 GCATGC
XbaI 2099 TCTAGA T^CTAGA
3164 TCTAGA
XcmI 1992 CCAGCTTCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2242 CCACACACTCAGTGG
XhoII 2110 AGATCC [GA]^GATC[CT]
3413 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I