##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AscI AseI AsnI Asp718I
AvrII BbrPI BbsI BciVI BclI
BfrI BlnI BpuAI BsaAI BsiWI
BsmI BspHI BssHII Bst1107I BstBI
BstZ17I CpoI EcoRI EcoT22I FbaI
FseI HincII HindII HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SalI SexAI
SfuI SgrAI SnaBI SpeI SspI
StuI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 1677 TCCGGA T^CCGGA
AflIII 1734 ACATGT A^C[GA][CT]GT
Alw44I 299 GTGCAC G^TGCAC
AocI 1561 CCTCAGG CC^T[ACGT]AGG
ApaLI 299 GTGCAC G^TGCAC
AspI 1511 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 1060 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2106 AGATCT A^GATCT
BsaBI 1840 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 328 GGTCTC GGTCTC(1/5)
BseAI 1677 TCCGGA T^CCGGA
BsmBI 4010 GAGACG CGTCTC(1/5)
BspDI 659 ATCGAT AT^CGAT
BspEI 1677 TCCGGA T^CCGGA
BspLU11I 1734 ACATGT A^CATGT
BssSI 808 CACGAG CACGAG(-5/-1)
Bsu36I 1561 CCTCAGG CC^T[ACGT]AGG
ClaI 659 ATCGAT AT^CGAT
DraIII 3635 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1400 GACACGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 710 CGGCCG C^GGCCG
EclXI 710 CGGCCG C^GGCCG
Eco52I 710 CGGCCG C^GGCCG
Eco81I 1561 CCTCAGG CC^T[ACGT]AGG
EcoRV 2243 GATATC GAT^ATC
HindIII 3684 AAGCTT A^AGCTT
KspI 2719 CCGCGG CCGC^GG
MamI 1840 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1677 TCCGGA T^CCGGA
PaeR7I 2366 CTCGAG C^TCGAG
PflFI 1511 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4039 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1941 CGATCG CGAT^CG
SacII 2719 CCGCGG CCGC^GG
SapI 2182 GCTCTTC GCTCTTC(1/4)
SbfI 85 CCTGCAGG CCTGCA^GG
ScaI 2196 AGTACT AGT^ACT
SfiI 1059 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2169 GCATGC GCATG^C
Sse8387I 85 CCTGCAGG CCTGCA^GG
TfiI 3389 GATTC G^A[AT]TC
Tth111I 1511 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2947 TCTAGA T^CTAGA
XhoI 2366 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AhdI 707 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1889 GACCATGTGTC
AspEI 707 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1889 GACCATGTGTC
AviII 12 TGCGCA TGC^GCA
210 TGCGCA
BalI 1086 TGGCCA TGG^CCA
2820 TGGCCA
BamHI 1857 GGATCC G^GATCC
1946 GGATCC
BcgI 2464 CGACAGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3538 GCACTGTCCTCG
BlpI 1098 GCTCAGC GC^T[ACGT]AGC
1237 GCTCAGC
Bpu1102I 1098 GCTCAGC GC^T[ACGT]AGC
1237 GCTCAGC
BsaWI 1677 TCCGGA [AT]^CCGG[AT]
2286 ACCGGA
BsiEI 710 CGGCCG CG[GA][CT]^CG
1941 CGATCG
BstAPI 847 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2173 GCAGGTCCTGC
CelII 1098 GCTCAGC GC^T[ACGT]AGC
1237 GCTCAGC
DraI 2963 TTTAAA TTT^AAA
3126 TTTAAA
Eam1105I 707 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1889 GACCATGTGTC
FspI 12 TGCGCA TGC^GCA
210 TGCGCA
MluNI 1086 TGGCCA TGG^CCA
2820 TGGCCA
MscI 1086 TGGCCA TGG^CCA
2820 TGGCCA
NaeI 1449 GCCGGC GCC^GGC
2010 GCCGGC
NcoI 1542 CCATGG C^CATGG
2634 CCATGG
NgoMIV 1449 GCCGGC G^CCGGC
2010 GCCGGC
SacI 71 GAGCTC GAGCT^C
2363 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SspBI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI